Cryopreserved rat cortical cells develop functional neuronal networks on microelectrode arrays

Otto, F.; Görtz, Philipp; Fleischer, Wiebke; Siebler, Mario

doi:10.1016/s0165-0270(03)00186-9

Cited by 49 publications

(40 citation statements)

References 38 publications

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“…To test the direct neurotoxicity of the polyols in vitro, we have used a 'neurochip' model with embryonic cortical neurons of the rat (Otto et al 2003). Vials of cryopreserved neurons (CryoCell Rat Brain Cortex Cells, QBM Cell Science, Ottawa, ONT, Canada) were stored in liquid nitrogen for up to 18 months.…”

Section: Discussionmentioning

confidence: 99%

“…Vials of cryopreserved neurons (CryoCell Rat Brain Cortex Cells, QBM Cell Science, Ottawa, ONT, Canada) were stored in liquid nitrogen for up to 18 months. After thawing, the cells were diluted with prewarmed B27-supplemented neurobasal medium (Invitrogen, Karlsruhe, Germany) and subsequently plated at densities from 1.5 to 4 × 10 5 /cm 2 onto poly-D-lysine-and laminin-coated MEAs with a square grid of 60 electrodes (30 µm diameter, 200 µm spacing) purchased from Multi Channel Systems, Reutlingen, Germany (Otto et al 2003). They developed a spontaneous active neuritic network within a few days in vitro.…”

Section: Discussionmentioning

confidence: 99%

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Influence of D‐arabitol and ribitol on neuronal network activity

Klusmann

Fleischer

Waldhaus

et al. 2005

J of Inher Metab Disea

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To date, two new defects in the pentose phosphate pathway have been identified in patients with abnormalities in their polyol profiles. Some of them presented with neurological symptoms of so far unknown aetiology. The pathophysiological role of polyols, e.g. in the brain, is relatively unknown. We tested the neurotoxicity of polyols using a 'neurochip' model. After exposure of cortical rat neurons to D-arabitol and ribitol in increasing concentrations up to 10 mmol/L, the electrophysiological activity was measured. No acute effect on the spontaneous network activity of cortical neurons was observed. We speculate that polyols have only secondary effects on brain dysfunction.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of D‐arabitol and ribitol on neuronal network activity

Klusmann

Fleischer

Waldhaus

et al. 2005

J of Inher Metab Disea

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show abstract

“…One of the methods to solve this problem is a cryopreservation of dissociated cells, which would supply cells in the same state whenever the researchers require them. Although cryopreservation of embryonic cells has been widely available (1,2), only a few studies have succeeded in cryopreserving cells from postnatal animals (3,4). This is probably because dissociated cells from postnatal animals require high nutrients and much oxygen, being more vulnerable to surgical procedures.…”

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confidence: 99%

Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus

et al. 2007

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Abstract. Although primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as long as 60 days of cryopreservation, GCs expressed the mature neuronal marker MAP-2 and elongated single tau-1-positive axons and multiple tau-1-negative dendrites. These properties closely resembled intact GCs in primary cultures, providing the advantage of being able to repeatedly prepare stable cultures with a single sacrifice of animals.

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“…Electrophysiological measurements using multielectrode arrays (MCS, Reutlingen/Germany) and cultures of cryopreserved cortical neurons of the embryonic rat (QBM, Ontario, Canada) were performed as described previously [24]. The rate of spontaneous action potentials, burst activity and interburst time intervals were calculated using the software SpANER (Result GmbH/Tö nnisvorst/Germany).…”

Section: Electrophysiologymentioning

confidence: 99%

“…Cultures of cortical neurons were exposed to increasing concentrations of recombinant AldC (100 nM-10 lM) in the culture medium. These neuronal networks develop spontaneous rhythmic discharges which were recorded by an extracellular electrode array [24]. After addition of AldC to the culture supernatant the rate of spontaneous action potentials decreased significantly in a reversible manner ( Fig.…”

Section: Influence Of Extracellular Aldolase C On the Spontaneous Elementioning

confidence: 99%

Aldolase C/Zebrin II is Released to the Extracellular Space after Stroke and Inhibits the Network Activity of Cortical Neurons

et al. 2006

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Cell death after stroke involves apoptotic, autophagocytic and necrotic mechanisms which may cause the release of cytosolic proteins to the extracellular space. Aldolase C (AldC) is the brain specific isoform of the glycolytic enzyme fructose-1,6-bisphosphate aldolase. According to its characteristic striped expression pattern in the adult cerebellum AldC is also termed zebrin II. Here, we demonstrate release of AldC into the cerebrospinal fluid (CSF) after stroke in vivo. Studies with cell cultures confirmed that AldC is released to the extracellular space after hypoxia. Moreover, addition of purified recombinant AldC to networks of cortical neurons plated on multielectrode arrays reversibly inhibited the spontaneous generation of action potentials at AldC concentrations which can be expected to occur after lesions of the human cerebral cortex. This mechanism could be relevant in the pathogenesis of the electrophysiological changes in the penumbra region after stroke.

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Cryopreserved rat cortical cells develop functional neuronal networks on microelectrode arrays

Cited by 49 publications

References 38 publications

Influence of D‐arabitol and ribitol on neuronal network activity

Influence of D‐arabitol and ribitol on neuronal network activity

Cryopreservation of Granule Cells From the Postnatal Rat Hippocampus

Aldolase C/Zebrin II is Released to the Extracellular Space after Stroke and Inhibits the Network Activity of Cortical Neurons

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