Cryopreserved human umbilical cord versus biocellulose film for prenatal spina bifida repair in a physiologic rat model

Snowise, Saul; Mann, Lovepreet K.; Morales, Yisel; Moise, Kenneth J.; Johnson, Anthony; Fletcher, Stephen; Grill, Raymond J.; Tseng, Scheffer C.G.; Papanna, Ramesha

doi:10.1002/pd.5035

Cited by 7 publications

(1 citation statement)

References 40 publications

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“…19,20,43 A particular patch derived from human umbilical cord promoted native cellular migration with minimal inflammatory response. 44 Other investigators have focused on the use of stem cells as an approach to facilitate/expedite skin closure. Intra-amniotic injections of amniotic or placental fluid derived mesenchymal stem cells induced partial or complete coverage of MMC defects and a reduction in Chiari II formation in a retinoic acideinduced rat model of MMC.…”

Section: Discussionmentioning

confidence: 99%

An Injectable Reverse Thermal Gel for Minimally Invasive Coverage of Mouse Myelomeningocele

Bardill

Williams

Shabeka

et al. 2019

Journal of Surgical Research

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Background: Myelomeningocele (MMC) results in lifelong neurologic and functional deficits. Currently, prenatal repair of MMC closes the defect, resulting in a 50% reduction in postnatal ventriculoperitoneal shunting. However, this invasive fetal surgery is associated with significant morbidities to mother and baby. We have pioneered a novel reverse thermal gel (RTG) to cover MMC defects in a minimally invasive manner. Here, we test in-vitro RTG long-term stability in amniotic fluid and in vivo application in the Grainy head-like 3 (Grhl3) mouse MMC model. Materials and methods: RTG stability in amniotic fluid (in-vitro) was monitored for 6 mo and measured using gel permeation chromatography and solutionegel transition temperature (lower critical solution temperature). E16.5 Grhl3 mouse fetuses were injected with the RTG or saline and harvested on E19.5. Tissue was assessed for RTG coverage of the gross defect and inflammatory response by immunohistochemistry for macrophages. Results: Polymer backbone molecular weight and lower critical solution temperature remain stable in amniotic fluid after 6 mo. Needle injection over the MMC of Grhl3 fetuses successfully forms a stable gel that covers the entire defect. On harvest, some animals demonstrate >50% RTG coverage. RTG injection is not associated with inflammation. Conclusions: Our results demonstrate that the RTG is a promising candidate for a minimally invasive approach to patch MMC. We are now poised to test our RTG patch in the large preclinical ovine model used to evaluate prenatal repair of MMC.

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