Cryopreserved and lyophilized cultured epidermal allografts in the treatment of leg ulcers: a pilot study

Navrátilová, Zuzana; Slonková, Veronika; Semrádová, Věra; Adler, Jiří

doi:10.1111/j.1468-3083.2004.00873.x

Cited by 28 publications

(12 citation statements)

References 35 publications

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“…Once these disadvantages are overcome, the market potential of CES is expected to rise tremendously due to their outstanding efficacy. A few studies have described the clinical application of L-CES to skin ulcers as an alternative to F-CES [9, 10]. However, those studies are limited in that L-CES have never been characterized in vitro in terms of their structural integrity or biological characteristics.…”

Section: Discussionmentioning

confidence: 99%

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Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES

Jang¹,

Kim²,

Kim

et al. 2013

BioMed Research International

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Lyophilized Cultured Epidermal Sheets (L-CES) have been reported to be as effective as the cryopreserved CES (F-CES) in treating skin ulcers. However, unlike F-CES, no preclinical study assessing wound-healing effects has been conducted for L-CES. The present study was set out to investigate the microstructure, cytokine profile, and wound-healing effects of L-CES in comparison to those of F-CES. Keratinocytes were cultured to prepare CES, followed by cryopreservation at −70°C and lyophilization. Under microscopic observation, intact cells with apparent intracellular junctions were observed in L-CES. The L-CES, like fresh CES, consisted of three to four well-maintained epidermal layers, as shown by the expression of keratins, involucrin, and p63. There were no differences in the epidermal layer or protein expression between L-CES and F-CES, and both CES were comparable to fresh CES. TGF-α, EGF, VEGF, IL-1α, and MMPs were detected in L-CES at levels similar to those in F-CES. In a mouse study, wounds treated with L-CES or F-CES completely healed at least 4 days faster than untreated wounds. CES-treated wounds completely healed by day 10, while the untreated wounds did not heal by day 14. Masson's trichrome staining showed that collagen deposition in the CES-treated wounds was highly increased in the dermis of the wound center compared to that in the control wounds. Thus, this study demonstrates that L-CES is as clinically effective as F-CES for wound treatment.

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Section: Discussionmentioning

confidence: 99%

“…Furthermore, L-CES were also shown to successfully treat chronic leg ulcers [9, 10]. Because lyophilization seems to provide a convenient and cost-effective way to prepare ready-made CES on a large scale, we were curious to know whether the wound-healing effects of L-CES are comparable to F-CES.…”

Section: Introductionmentioning

confidence: 99%

Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES

Jang¹,

Kim²,

Kim

et al. 2013

BioMed Research International

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“…In other studies, wound healing varied between 26 and 39Á9 days after transplantation of allogenic cryopreserved cultured keratinocytes. The size of the ulcer was mainly reduced rapidly within the first to fourth week (10)(11)(12)(13)(14). The authors believe that this is not only because of the release of different cytokines and growth factors by the transplanted keratinocytes but probably also because of deposition of extracellular matrix and basement membrane components.…”

Section: Key Pointsmentioning

confidence: 96%

Mathematical model for wound healing following autologous keratinocyte transplantation

Renner

Teuwen

Gebhardt

et al. 2008

International Wound Journal

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In times of increasing economical pressure on the health care systems, it is important to optimise the outpatient treatment of chronic wounds. Another aim of wound healing research is to discover agents to accelerate healing. Wound healing trajectories or healing velocities can provide information to demonstrate the endpoints for wound healing. A great problem in clinical trials is to specify these parameters. Therefore, we developed a mathematical model for more transparency. In this initial project, we observed 19 wounds to construct the wound healing trajectories after transplantation of autologous keratinocytes, and the results are so encouraging that investigation in this area will continue. The developed mathematical model describes the clinical observed healing process. It was possible to find parameters to distinguish between old and young patients, retrospectively or prospectively calculate the healing rates and to determine exactly the endpoint of healing. Therefore, our model might be very useful in practices or for studies.

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“…The authors advocated the more frequent use of lyophilized epidermal allografts because they may be conveniently stored at room temperature. 109 There are no randomized controlled trials comparing cultured epidermal allografts with other biologic or nonbiologic dressings for venous leg ulcers, diabetic ulcers, or acute surgical wounds ( Table 2).…”

Section: Epidermal Graftsmentioning

confidence: 99%

“…At 3 months, there was no statistically significant difference in the rate of completely healed ulcers in the cryopreserved group (84%) and the lyophilized group (80%). The authors advocated the more frequent use of lyophilized epidermal allografts because they may be conveniently stored at room temperature 109 …”

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confidence: 99%

Biologic Dressings

Chern

Baum

Arpey

2009

Dermatologic Surgery

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There is extensive literature regarding the use of biologic dressings in chronic wounds, such as venous leg ulcers and burns, but studies evaluating these dressings in acute surgical wounds and dermatologic surgery have been limited. There appear to be specific surgical settings in which such dressings may be of particular use, in addition to limitations of their use. Additional studies, particularly randomized and comparative trials, would be highly desirable.

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Cryopreserved and lyophilized cultured epidermal allografts in the treatment of leg ulcers: a pilot study

Cited by 28 publications

References 35 publications

Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES

Wound-Healing Potential of Cultured Epidermal Sheets Is Unaltered after Lyophilization: A Preclinical Study in Comparison to Cryopreserved CES

Mathematical model for wound healing following autologous keratinocyte transplantation

Biologic Dressings

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