Cryopreservation of umbilical cord blood with a novel freezing solution that mimics intracellular ionic composition

Abstract

Slow and fast addition of cryopreservation solutions result in mean temperature changes of approximately 3.3 to 4.45°C. Postthaw recoveries with CryoStor were equivalent to or slightly better than with the in-house cryopreservation solution. CryoStor also provides several advantages including reduced processing time, formulation consistency, and reduced DMSO in the frozen product (≤ 5%).

Relevance of flow cytometric enumeration of post‐thaw leucocytes: influence of temperature during cell staining on viable cell recovery

“…reported higher CD34 recoveries when using a synthetic freezing solution (CryoStor CS10) compared to their standard media. . Analogous results were obtained in our own experiments .…”

Comparison of the cryoprotective solutions based on human albumin vs. autologous plasma: its effect on cell recovery, clonogenic potential of peripheral blood hematopoietic progenitor cells and engraftment after autologous transplantation

“…The results were based on evaluation of nine samples. Such intracellular‐like cryopreservation solutions in comparison with isotonic‐based freeze media were also a subject of studies concerning cryopreservation of different cell types, like cord blood , MSCs , PBMCs and other in vitro cell models . However, because of using different final concentrations of DMSO, none of these studies did compare directly the impact of DMSO diluent on cells’ quality, but rather completely different cryoprotectant mixtures.…”

“…The results were based on evaluation of nine samples. Such intracellularlike cryopreservation solutions in comparison with isotonic-based freeze media were also a subject of studies concerning cryopreservation of different cell types, like cord blood [38,39], MSCs [40], PBMCs [41] and other Fig. 1 Effect of human serum albumin solution (HSAS) vs. autologous plasma (AP) on cell recovery.…”

Effects of cryopreservation on excretory function, cellular adhesion molecules and vessel lumen formation in human umbilical vein endothelial cells

“…After 0, 4, 8, 12 or 24 weeks of cryopreservation in liquid nitrogen at −196°C, the HUVECs were thawed in a 37°C water bath for 1–2 min. Oscillation of the cryopreserved tube was performed until the cell suspension had completely thawed (12). …”