Cryopreservation of turbot () spermatozoa

Dréanno, Catherine; Suquet, Marc; Quéméner, Loïc; Cosson, Jacky; Fierville, F.; Normant, Yvon; Billard, Roland

doi:10.1016/s0093-691x(97)00276-8

Cited by 93 publications

(90 citation statements)

References 26 publications

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“…Similar results for the motility parameters of frozen-thawed spermatozoa were reported in some experiments (14,15,20). However, different results were determined in cryopreserved turbot sperm (9), where post-thaw motility was significantly lower than that of fresh sperm.…”

Section: Resultssupporting

confidence: 83%

Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon Idella) Sperm

Yavaş

Bozkurt

2011

Biotechnology & Biotechnological Equipment

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Section: Resultssupporting

confidence: 83%

Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon Idella) Sperm

Yavaş

Bozkurt

2011

Biotechnology & Biotechnological Equipment

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“…In general, sperm motility reduced between 10 -70% on average in the species studied (Table 4). These observations are similar to studies in several other species such as Cyprinus carpio L. (Wamecke & Pluta, 2003), Oncorhynchus mykiss (Lahnsteiner et al, 1996) and Scophthalmus maximus (Dreanno et al, 1997 For all Malaysian fish species studied, the post-thaw motility rates of the cryopreserved sperm demonstrated similar values (p>0.05) even after a year of cryostorage as long as the semen samples are submerged well in the liquid nitrogen and without disturbance during the storage period.…”

Section: Quality Of Semensupporting

confidence: 86%

Sperm Cryopreservation of Some Freshwater Fish Species in Malaysia

Chew¹,

Zulkafli²

2012

Current Frontiers in Cryopreservation

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“…It is difficult to correlate with the previously published results of 90 nmol/10 9 spermatozoa by Dreanno et al (1999b) due to the difference of unit. Dreanno et al (1997) and Zilli et al (2004) have proposed to use ATP as a sperm quality marker for sperm used for cryoconservation.…”

Section: Analysis Of Marine Fish Spermatozoamentioning

confidence: 99%

Marine fish spermatozoa: racing ephemeral swimmers

Cosson

Groison²,

Suquet³

et al. 2008

Reproduction

144

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After a long period of spermatogenesis (several weeks to months), marine fish spermatozoa are delivered at male spawning in seawater (SW) at the same time as ova. In some fish species, as the ova micropyle closes quickly after release, these minute unicells, the spermatozoa, have to accomplish their task of reaching the micropyle within a very brief period (several seconds to minutes), for delivery of the haploid male genetic information to the ova. To achieve this goal, their high-performance motile equipment, the flagellum, must fully activate immediately on contact with the SW and then propel the sperm cell at an unusually high initial velocity. The cost of such 'hyperactivity' is a very rapid consumption of intracellular ATP that outstrips the supply. The spermatozoa become rapidly exhausted because mitochondria cannot compensate for this very fast flagellar energy consumption. Therefore, any spermatozoon ends up with two possibilities: either becoming exhausted and immotile or reaching the egg micropyle within its very short period of forward motility (in the range of tens of seconds) before micropyle closure in relation to both contact of SWand cortical reaction. The aim of the present review is to present step by step the successive events occurring in marine fish spermatozoa from activation until their full arrest of motility. The present knowledge of activation mechanisms is summarized, as well as a description of the motility parameters characterizing the motility period. As a complement, in vitro results on axonemal motility obtained after demembranation of flagella bring further understanding. The description of the sperm energetic content (ATP and other high energy compounds) and its evolution during the swimming period is also discussed. A general model aiming to explain all the successive cellular events occurring immediately after the activation is presented. This model is proposed as a guideline for understanding the events governing the sperm lifespan in the marine fish species that reproduce through external fertilization.

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Cryopreservation of turbot () spermatozoa

Cited by 93 publications

References 26 publications

Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon Idella) Sperm

Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon Idella) Sperm

Sperm Cryopreservation of Some Freshwater Fish Species in Malaysia

Marine fish spermatozoa: racing ephemeral swimmers

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