Cryopreservation of Tropical Plant Germplasm with Vegetative Propagation - Review of Sugarcane (Saccharum spp.) and Pineapple (Ananas comusus (L.) Merrill) Cases

Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

“…To cryopreserve pineapple shoot tips, the droplet-vitrification method was performed as described by Souza et al [10], with some modifications according to the studies of Martínez-Montero et al [5,9]. Specifically, plant vitrification solution 3 (PVS3; solution consisting of MS + 50% (w/v) sucrose + 50% (w/v) glycerol) [33] was used instead of PVS2 (solution consisting of MS + 30% (w/v) glycerol, 15% (w/v) ethylene glycol + 15% (w/v) dimethylsulfoxide + 0.4 M sucrose) [34].…”

“…In the case of important edible horticultural crops like pineapples (Ananas comosus var. comosus), which are vegetatively propagated and where crosses between varieties produce botanical seeds that are highly heterozygous, their seeds are of limited interest for the conservation of specific gene combinations [5]. The cryopreservation of shoot tips is the most relevant strategy for the long-term conservation of the pineapple crop.…”

“…Cryopreservation methods have already been developed for pineapple shoot tips [5,[7][8][9][10][11][12][13]. The vitrification-based cryopreservation method is the most widely applied for cryopreserving pineapple shoot tips [5,[10][11][12][13].…”

“…Cryopreservation methods have already been developed for pineapple shoot tips [5,[7][8][9][10][11][12][13]. The vitrification-based cryopreservation method is the most widely applied for cryopreserving pineapple shoot tips [5,[10][11][12][13]. In this method, precultured shoot tips are exposed to a highly concentrated plant vitrification solution (PVS) before being directly immersed in liquid nitrogen (LN) [2,12,13].…”

Etapas del protocolo de crioconservación y su efecto sobre la viabilidad y regeneración de embriones cigóticos de vid (Vitis vinífera L.)

“…Esto, es aún controversial, ya que algunos trabajos reportan que la crioconservación es una técnica eficiente, mientras que otros mencionan lo contrario. En este sentido, Martínez-Montero et al (2012) y Souza et al (2016) establecieron un protocolo de crioconservación para puntas de brote de Ananas comosus L., que no afectó el ren-DOI: 10.18633/biotecnia.v25i3.1991 dimiento en campo de las puntas de brote crio conservadas (Villalobos-Olivera et al, 2019). Bettoni et al (2019) reportaron el 50 -55 % de regeneración en puntas de brote crioconservadas de Vitis.…”