Cryopreservation of sperm of red snapper (Lutjanus campechanus)

Riley, Kenneth L.; Holladay, Chase G.; Chesney, Edward; Tiersch, Terrence R.

doi:10.1016/j.aquaculture.2004.04.025

Cited by 30 publications

(28 citation statements)

References 15 publications

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“…Wayman et al (1998) studied extenders with different osmolalities (200, 300 and 400 mOsm/kg) concluded that 200 mOsm/kg was the most suitable for preservation of Sciaenops ocellatus (Linnaeus, 1766) sperm. Extender with osmolarity of 200 mOsm already been used successfully in sperm cryoconservation of L. campechanus (Riley et al, 2004). This value is very close to the values of the osmolarity of the extender in this study, but contrast with the extender with osmolarity of 315 mOsm used in sperm cryoconservation of L. argentimaculatus (Vuthiphandchai et al, 2009).…”

Section: Dmso (%) Motility Rate (%) Motility Time (S)contrasting

confidence: 41%

“…The sperm of lane snapper examined after cryoconservation process, (Riley et al, 2004), but in contrast to that reported for other species of lutjanidae (L. argentimaculatus) from 1.3 to 2.8 × 10 10 (Vuthiphandchai et al, 2009). For lane snapper the Colombian coast, sperm density reported was 1.3 × 10 9 cells mL -1 (Gaitán-Espitia et al, 2013).…”

Section: Discussionmentioning

confidence: 50%

“…The results of this experiment indicated that for sperm cryoconservation of lane snapper, the cooling rate of 60°C -min showed a better performance, with a significant difference (P <0.05) of all velocities tested. This rate is considerably higher than that used in the sperm cryoconservation of L. campechanus 16 °C -min (0.5 mL vanes) (Riley et al, 2004), used in the sperm cryopreservation of L. argentimaculatus that employing 0.25 ml straws and different cooling rates (3, 5, 10 and 12 °C -min ) obtained the best results with 10 °C -min (Vuthiphandchai et al, 2009) and used to lane snapper sperm 15 °C -min (Gaitan-Espitia et al, 2013). However, the results obtained in our study agreed with those obtained by Chao et al (1992) who studied the sperm cryoconservation of Epinephelus malabaricus (Block and Schneider, 1801) obtained the best results using 60 °C -min and with those obtained by Miyaki et al (2005) who used this same cooling rate on sperm cryopreservation of Epinephelus moara (Block, 1793).…”

Section: Dmso (%) Motility Rate (%) Motility Time (S)mentioning

confidence: 88%

“…In this study the concentration of 10% DMSO was more effective in preserving the quality of lane snapper sperm. This same concentration was considered adequate for the sperm cryopreservation of L. campechanus and L. argentimaculatus (Riley et al, 2004;Vuthiphandchai et al, 2009).…”

Section: Dmso (%) Motility Rate (%) Motility Time (S)mentioning

confidence: 99%

“…Although sperm cryoconservation is an important strategy for the control of reproduction, few studies have focused on these techniques for species of lutjanidae, with emphasis results obtained with Lutjanus campechanus (Poey, 1860), with Lutjanus argentimaculatus (Forsskål, 1775) (Riley et al, 2004;Vuthiphandchai et al, 2009) and the mutton snapper L. analis (Sanches et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

et al. 2015

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This study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C -min ), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C Criopreservação do sêmen do ariocó Lutjanus synagris (Linnaeus, 1758) ResumoEste estudo teve a finalidade de desenvolver e avaliar um protocolo de crioconservação do sêmen do ariocó Lutjanus synagris. Para caracterizar o sêmen foram avaliados a taxa de motilidade, a duração da motilidade, a concentração espermática e o espermatócrito. Em seis experimentos foram analisados os efeitos de três diluentes, com distintas composições iônicas e valores de pH distintos, combinados com sete concentrações de dimetilsulfóxido (0; 2,5; 5,0; 7,5; 10,0; 12,5 e 15,0%), cinco velocidades de congelamento (-110, -90, -60, -45 e -30°C/min), nove tempos de equilíbrio (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutos) e cinco proporções de sêmen:diluente (1:1; 1:3; 1:6; 1:10 e 1:20) sobre a taxa de motilidade e a duração da motilidade espermáticas. Posteriormente um teste de fertilização foi realizado para avaliar a viabilidade do sêmen crioconservado. O tratamento que propiciou maior taxa de motilidade e duração da motilidade espermáticas (P<0,05) foi aquele proporcionado pelo emprego do diluente com pH 8,2 com dimetilsulfóxido a 10%, em uma velocidade de congelamento de -60°C/min, com tempo de equilíbrio de 1 minuto e na proporção de 1:3 (v/v). O sêmen crioconservado apresentou taxa de fertilização superior a 69% validando o presente protocolo para o ariocó. A crioconservação do sêmen do ariocó é uma alternativa viável, sendo possível manter uma apropriada qualidade espermática.

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Section: Dmso (%) Motility Rate (%) Motility Time (S)contrasting

confidence: 41%

Section: Discussionmentioning

confidence: 50%

Section: Dmso (%) Motility Rate (%) Motility Time (S)mentioning

confidence: 88%

Section: Dmso (%) Motility Rate (%) Motility Time (S)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

et al. 2015

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On‐Site Capabilities of a Mobile Laboratory for Aquatic Germplasm Cryopreservation

et al. 2019

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The cryopreservation of genetic material can be an important tool for researchers and others involved with imperiled fishes, wild fisheries, aquaculture, and biomedical research. The standardization and reliable collection of diverse, high‐quality samples are persistent challenges to the successful cryopreservation of aquatic species. The overall goal of this study was to work with different user groups, cryopreserving sperm on‐site at their facilities to evaluate the uses and challenges of a mobile laboratory with high‐throughput and quality control capabilities comparable to those of a specialized central facility. The objectives were to demonstrate the collection and cryopreservation of sperm from (1) large‐bodied freshwater Blue Catfish Ictalurus furcatus for aquaculture; (2) small‐bodied freshwater swordtails and platyfishes Xiphophorus spp. for biomedical research and repository development for gametes from imperiled species; and (3) saltwater Red Snapper Lutjanus campechanus for wild fisheries research. Over the course of this project, the mobile laboratory traveled more than 4,000 km, collecting germplasm from more than 650 male fish. A total of 137 Blue Catfish were processed in 2015 and 2016, yielding 6,146 0.5‐mL French straws. A total of 521 males from 11 different species in the genus Xiphophorus were processed over 4 d in 2015, yielding 488 0.25‐mL French straws. Lastly, a total of 17 Red Snapper males were processed during 2015, yielding 316 0.5‐mL French straws. This study documents the development of a mobile cryopreservation laboratory with high‐throughput capability for aquatic species. If mobile laboratories prove to be effective, user groups will no longer be limited to germplasm resources that can be shipped as samples or transported as live animals to a central cryopreservation facility. Mobile laboratories would thus create opportunities to collect higher‐quality germplasm and provide access to new species. Also, they would enable direct cooperation, including training, among a wide variety of user groups for numerous applications.

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