Cryopreservation of Specific Pathogen-Free (SPF) Pig Islet Cells: Effect of Culture Time before Cryopreservation and after Thawing

Duvivier, Valérie; Darquy, Sylviane; Réach, G.

doi:10.1006/cryo.1999.2181

Cited by 3 publications

(5 citation statements)

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“…It has been shown that 10-14 days are required to reestablish islet blood flow after transplantation (41,42) and cryopreservation of islets does not interfere with the vascularization process (42). On the other hand, Duvivier et al have shown the delayed functional recovery in cryopreserved islets (43). In their in vitro study, islets exhibited the highest insulin release response 14 days after thawing as compared to 3, 7 and 21 days.…”

Section: Omori Et Almentioning

confidence: 99%

Improvement of Human Islet Cryopreservation by a p38 MAPK Inhibitor

Omori

Valiente

Orr

et al. 2007

American Journal of Transplantation

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The activation of p38 mitogen-activated protein kinase (MAPK) has been shown to cause ischemia/reperfusion injury of several organs used for transplantation and also to play a significant role in primary islet graft nonfunction. Activation of p38 MAPK may also occur during islet cryopreservation and thawing. In this study, a p38 MAPK inhibitor (p38IH) was applied to human islet cryopreservation to improve islet yield and quality after thawing. Under serum-free conditions, human islets were cryopreserved, thawed and cultured using our standard procedures. Three types of solutions were tested: conventional RPMI1640 medium (RPMI), a newly developed islet cryopreservation solution (ICS), and ICS supplemented with a p38IH, SD-282 (ICS-p38IH). Activation or inhibition of p38 MAPK was demonstrated by the diminished phosphorylation of HSP27 substrate. Islet recovery on day 2 after thawing was highest with ICS-p38IH and islet viability was not significantly different in the three groups. b Cell numbers and function were the highest in islets cryopreserved with ICS-p38IH. Glucosestimulated human C-peptide levels were 86% of that of the nonfrozen islets when measured 4 weeks after transplantation into NODscid mice. This improvement may provide an opportunity to establish islet banks and allow the use of cryopreserved islets for clinical transplantation.

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Section: Omori Et Almentioning

confidence: 99%

Improvement of Human Islet Cryopreservation by a p38 MAPK Inhibitor

Omori

Valiente

Orr

et al. 2007

American Journal of Transplantation

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“…Lakey et al (1997) reported the recovery of islets after 48 h of tissue culture, following cryopreservation and thawing. Duvivier et al (1999) found that increasing the culture time (3, 7, 14 or 21 days) before and after cryopreservation/thawing of pig islet cells, improved insulin release in response to glucose. Gatto et al (2003) indicated that coculture of human islets with pancreatic ductal epithelial cells (DEC) represents a valuable tool to improve the survival and functional activity of islets, especially following cryopreservation/thawing.…”

Section: Discussionmentioning

confidence: 95%

“…The value of tissue culture before cryopreservation or after thawing has been suggested by several authors (McKay and Karow, 1983;Sandler and Andersson, 1987;Foreman and Taylor, 1989;Vasir et al, 1989;Kneteman et al, 1989;Foreman et al, 1993;Lakey et al, 1997;Duvivier et al, 1999;Gatto et al, 2003). McKay and Karow (1983) have found that islet function can be significantly improved by increasing the duration of post-thaw culture.…”

Section: Discussionmentioning

confidence: 99%

Effect of thawing rate and post-thaw culture on the cryopreserved fetal rat islets: Functional and morphological correlation

et al. 2006

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“…18 The optimal condition for islet cryopreservation in freezing and thawing periods are still under investigation. Some authors demonstrated that the culture time before freezing 7,19 or replacement of DMSO by other cryoprotectants 4 may decrease the adverse effects of cryopreservation. In our study, islet microencapsulation and supplementation of the culture media with sucrose was applied for overcoming the toxicity of DMSO.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the possibility of reduced pancreatic islet immunogenicity, the collection of sufficient numbers of encapsulated islets followed by cryopreservation and long-term banking was tested, showing that pig islet cells remained functional. 7 The aim of our study was to assess the influence of long-term storage at low temperature on functional capabilities of encapsulated islets derived from rat, porcine, and human pancreata.…”

Section: Introductionmentioning

confidence: 99%

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Kinasiewicz

Antosiak-Iwańska²,

Godlewska³

et al. 2018

Exp Clin Transplant

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Objectives: Immunoisolation of pancreatic islets of Langerhans performed by the encapsulation process may be a method to avoid immunosuppressive therapy after transplant. The main problem related to islet transplant is shortage of human pancreata. Resolution of this obstacle may be cryopreservation of encapsulated islets, which enables collection of sufficient numbers of isolated islets required for transplant and long-term storage. Here, we assessed the ability of encapsulated islets to function after longterm banking at low temperature. Materials and Methods: Islets of Langerhans isolated from rat, pig, and human pancreata were encapsulated within alginate-poly-L-lysine-alginate microcapsules. Cryopreservation was carried out using a controlled method of freezing (Kriomedpol freezer; Kriomedpol, Warsaw, Poland), and samples were stored in liquid nitrogen. After 10 years, the samples were thawed with the rapid method (with 0.75 M of sucrose) and then cultured. Results: We observed that microcapsules containing islets maintained their shape and integrity after thawing. During culture, free islets were defragmented into single cells, whereas encapsulated islets were still round in shape and compact. After 1, 4, and 7 days of culture of encapsulated islets, the use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests showed increased mitochondrial activity. After they were thawed, the insulin secretion capacity was comparable with that obtained with fresh islets.Conclusions: Cryopreservation and storage of free and microencapsulated islets were possible for about 10 years, although only encapsulated islets retained viability and secretory properties.

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Cryopreservation of Specific Pathogen-Free (SPF) Pig Islet Cells: Effect of Culture Time before Cryopreservation and after Thawing

Cited by 3 publications

References 0 publications

Improvement of Human Islet Cryopreservation by a p38 MAPK Inhibitor

Improvement of Human Islet Cryopreservation by a p38 MAPK Inhibitor

Effect of thawing rate and post-thaw culture on the cryopreserved fetal rat islets: Functional and morphological correlation

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