Cryopreservation of shoot tips, evaluations of vegetative growth, and assessments of genetic and epigenetic changes in cryo-derived plants of Actinidia spp.

Zhang, Xiaochen; Bao, Wen-Wu; Zhang, A-Ling; Pathirana, Ranjith; Liu, Zhande

doi:10.1016/j.cryobiol.2020.05.004

Cited by 17 publications

(11 citation statements)

References 51 publications

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“…Some studies on in vitro culture related to conservation procedures (slow growth and cryopreservation) do not report how many DNA samples from each experimental condition were used to perform the MSAP analysis. In some cases, a single sample was used per experimental condition, it being sometimes a pool of several samples [ 111 , 121 , 122 , 123 ]. Or the fragments obtained from the different analyzed samples were counted together without considering the variability between the samples within each experimental condition.…”

Section: Statistical Methods For Msap Analysis In Plant Conservatimentioning

confidence: 99%

“…In most of these studies, only a description of methylation events and/or changes in methylation between different conditions was stated [ 111 , 121 , 122 , 124 ]. A statistical analysis of this type of samples would only reflect the variability between fragments for the different experimental conditions but does not allow to compare it with the experimental error.…”

Section: Statistical Methods For Msap Analysis In Plant Conservatimentioning

confidence: 99%

“…Zhang et al [ 111 ] compared the DNA methylation status of kiwi ( Actinidia chinensis Planch.) plants originated from cryopreserved (by vitrification) apices to those from in vitro multiplication.…”

Section: Cryopreservationmentioning

confidence: 99%

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Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

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Section: Statistical Methods For Msap Analysis In Plant Conservatimentioning

confidence: 99%

Section: Statistical Methods For Msap Analysis In Plant Conservatimentioning

confidence: 99%

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Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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“…Those authors reported that following 10 years of slow growth in vitro, there were minimal incidences of somaclonal variants (as assessed in eld trials over two growing seasons). In other studies, vegetative growth and plant characteristics following cryopreservation have been assessed under glasshouse conditions, for example in shallot (Wang et al 2021) and kiwifruit (Zhang et al 2020). However, it is important to assess the resilience of cryostored plants when grown under eld conditions as this is ultimately the system used for production.…”

Section: Resultsmentioning

confidence: 99%

Storage of Pineapple Shoot Tips in Liquid Nitrogen for Three Years Does Not Modify Field Performance of Recovered Plants

Villalobos-Olivera¹,

Entensa²,

Martínez³

et al. 2021

Preprint

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Pineapple is the third most important tropical fruit traded after banana and mango. In recent years, pineapple producers have faced production challenges due to unpredictable weather patterns as a consequence of climate change. In order to develop new genotypes with improved tolerance to these biotic and abiotic stresses, it is necessary to have access to a diverse gene pool, however, the germplasm currently stored in field gene banks are at risk from prevailing environmental conditions. Therefore, there is a need to develop and implement supplementary strategies to conserve plant germplasm under controlled conditions in the laboratory and cryopreservation represents the most viable option for this. To date, methods have been developed for cryopreservation of pineapple shoot tips but there is a lack of information on whether extended periods of storage in liquid nitrogen (LN) has any effect on the regeneration capacity and field performance of cryostored germplasm. Hence, the current study investigated the field performance of pineapple shoot tips after storage for 24 h, one, two and three years. Results were collected following nine months for plant morphological characteristics and 14 months for evaluation of fruit and nutritional characteristics. The results indicated that storage of pineapple shoot tips for up to three years did not have any adverse effects on field performance of plants or on fruit characteristics. This provides evidence that cryopreservation offers a suitable tool for the long term storage of germplasm.

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“…Such reversible epigenetic mechanism has also been noted in the regenerants recovered from cryopreserved shoot tips of Carica papaya [ 65 ], Mentha × piperita [ 70 ], and Actinidia chinensis var. deliciosa [ 71 ], and from the cryopreserved somatic embryos of Bactris gasipaes [ 72 ] and Theobroma cacao [ 66 ]. As speculated by Harding et al [ 54 ], changes in DNA methylation may be an adaptive response to oxidative stress induced during cryopreservation.…”

Section: Assessments Of Epigenetic and Genetic Integritymentioning

confidence: 99%

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Shukla

Ren

et al. 2021

Plants

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Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

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Cryopreservation of shoot tips, evaluations of vegetative growth, and assessments of genetic and epigenetic changes in cryo-derived plants of Actinidia spp.

Cited by 17 publications

References 51 publications

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Storage of Pineapple Shoot Tips in Liquid Nitrogen for Three Years Does Not Modify Field Performance of Recovered Plants

Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

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