Cryopreservation of seeds and protocorms of rare temperate orchids

Nikishina, T. V.; Попова, Елена; Vakhrameeva, M. G.; Варлыгина, Т.И.; Коломейцева, Г. Л.; Burov, Alexander; Popovich, E. A.; Shirokov, A. I.; Shumilov, V. Yu.; Popov, A. S.

doi:10.1134/s1021443707010189

Cited by 54 publications

(30 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When evaluating the effect of cryopreservation on seeds of rare tropical orchids, Nikishina et al (2007) concluded that, after the storage on liquid nitrogen, the germination capacity of the seeds increased for two species and decreased for other two species.…”

Section: Resultsmentioning

confidence: 99%

Seed cryopreservation of Passiflora species

et al. 2016

View full text Add to dashboard Cite

-The aim of this study was to evaluate the cryopreservation of seeds of Passiflora species, using different cryoprotectants. The completely randomized design was used with four treatments (T1 -DMSO at 7%; T2 -0.3 M sucrose; T3 -storage in NL 2 without cryoprotectants; T4 -control) and five replications of 50 seeds. The seeds were stored for 120 hours in NL 2 (-196 °C), and thawing was conducted in a water bath (37 °C) for 20 minutes, proceeding with the germination and vigor tests. The data were submitted to ANOVA and Tukey's test (5% of probability) using the SISVAR program. In P. mucronata, P. suberosa and P. edulis seeds, the responses obtained were favorable to cryopreservation without the use of cryoprotectants in regards to P. micropetala, there was a considerable loss of germination potential after storage in NL 2 . Given these circunstances, cryopreservation can be used for long-term conservation of Passiflora mucronata, P. suberous and P. edulis, without the use of cryoprotectants.Index terms: passion fruit, storage, germination.Criopreservação de sementes de espécies de Passiflora RESUMO: Objetivou-se avaliar a criopreservação de sementes de espécies de Passiflora, utilizando-se diferentes crioprotetores. Foi utilizado o Delineamento Inteiramente Casualizado, com quatro tratamentos (T 1 -DMSO a 7%; T 2 -sacarose a 0,3 M; T 3 -armazenamento em NL 2 sem crioprotetor; T 4 -controle) e cinco repetições de 50 sementes. As sementes foram armazenadas durante 120 horas em NL 2 (-196 °C), realizou-se o descongelamento em banho-maria (37 °C) durante 20 minutos, e prosseguiram-se os testes de germinação e vigor. Os dados foram submetidos à análise de variância e teste de Tukey (5% probabilidade) pelo programa SISVAR. Em sementes de P. mucronata, P. suberosa e P. edulis foram obtidas respostas favoráveis à criopreservação sem o uso de crioprotetores, e em P. micropetala houve perda considerável do potencial germinativo após armazenamento em NL 2 . Diante disso, a criopreservação pode ser utilizada para conservação em longo prazo de sementes de Passiflora mucronata, P. suberosa e P. edulis, sem o uso de crioprotetores.Termos para indexação: maracujá, armazenamento, germinação.

show abstract

Section: Resultsmentioning

confidence: 99%

Seed cryopreservation of Passiflora species

et al. 2016

View full text Add to dashboard Cite

show abstract

“…This is achieved by the addition of cryoprotective solutions, such as glycerol, dimethylsulfoxide, glycols, and plant vitrification solution 2 (PVS2), a mixture of 30% (w/v) glycerol, 15% (w/v) ethylene glycol, and 15% (w/v) dimeth-54 INTERNATIONAL JOURNAL OF PLANT SCIENCES ylsulfoxide (Sakai and Engelmann 2007). Vitrification techniques-developed principally to allow for the cryopreservation of desiccation-sensitive plant material, such as in vitro tissue culture-derived shoot tips, somatic embryos, and cell cultures-are becoming increasingly common for the cryostorage of orchid seeds and protocorms (Ishikawa et al 1997;Hirano et al 2005Hirano et al , 2011Nikishina et al 2007;Vendrame et al 2007;Huehne and Bhinija 2011;Galdiano et al 2012;Watanawikkit et al 2012). Vitrification appears to enhance survivability of orchid seeds.…”

Section: Vitrificationmentioning

confidence: 99%

Ex situ Conservation and Cryopreservation of Orchid Germplasm

Merritt

Hay

Swarts

et al. 2014

International Journal of Plant Sciences

View full text Add to dashboard Cite

Premise of research. Orchids are among the most enigmatic of plant species. Yet the Orchidaceae comprises more species at risk of extinction than any other plant family. The collection and storage of orchid germplasmprincipally seeds and associated mycorrhizal fungi but also protocorm-like bodies using encapsulation and vitrification techniques-allows for secure ex situ conservation. This article reviews the approaches and techniques used for the ex situ conservation of orchid germplasm, with a focus on seed banking and the use of cryopreservation techniques to improve the longevity of germplasm.Pivotal results. It is increasingly apparent that cryopreservation-the storage of germplasm at ultra-low temperatures (e.g., in liquid nitrogen)-is required for the long-term and low-maintenance conservation of all types of orchid germplasm. For orchid seeds, desiccation tolerance is common, but longevity in storage is poor. Cryopreservation of orchid seeds shows promise, but some complexities in low-temperature storage behavior still require explanation and resolution. The application of more advanced cryopreservation techniques, including encapsulation-dehydration and vitrification, is becoming increasingly common. These techniques provide for the simultaneous storage of orchid propagules with their compatible fungus, while for seeds, vitrification techniques show potential for improving tolerance to the stresses of cryopreservation. Conclusions.A renewed focus on describing the low-temperature storage physiology of orchid seeds to more precisely define the relationship between seed water content, storage temperature, and seed survival is required, as is perhaps the wider adoption of the use of cryoprotectants for seeds. This research, coupled with the development of improved methods of seed viability testing, will support the growing work of germplasm banks to protect orchid biodiversity in the face of habitat loss and potential species extinction.

show abstract

“…rubro oculata Paxt., Encyclia cochleata, Angraecum magdalenae, Miltonia flavescens × Brassia longissima, and Trichopilia tortilis were stored in a refrigerator for 7 days to 7 years prior to cryopreservation [36]. Harvested seeds of Dactylorhiza balitica, D. fuchsii, Dactylorhiza incarnata, and Dactylorhiza maculata were stored at 5-6°C for 2-3 weeks, then directly immersed in LN [37]. Harvested seeds of Disa uniflora, Eulophia Alta, Eulophia streptopetala, Satyrium nepalense var.…”

Section: Desiccation Methodsmentioning

confidence: 99%

Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana

RungYi¹,

Chang²,

Hsieh³

et al. 2016

Cryopreservation in Eukaryotes

View full text Add to dashboard Cite

Many native orchid populations declined yearly due to economic development and climate change. This resulted in some wild orchids being threatened. In order to maintain the orchid genetic resources, development of proper methods for the long-term preservation is urgent. Low temperature or dry storage methods for the preservation of orchid genetic resources have been implemented but are not effective in maintaining high viability of certain orchids for long periods. Cryopreservation is one of the most acceptable methods for long-term conservation of plant germplasm. Orchid seeds and pollens are ideal materials for long-term preservation (seed banking) in liquid nitrogen (LN) as the seeds and pollens are minute, enabling the storage of many hundreds of thousands of seeds or pollens in a small vial, and as most species germinate readily, making the technique very economical. This article describes cryopreservation of orchid genetic resources by desiccation and a case study of Bletilla formosana. We hope to provide a more practical potential cryopreservation method for future research needs.

show abstract

Cryopreservation of seeds and protocorms of rare temperate orchids

Cited by 54 publications

References 3 publications

Seed cryopreservation of Passiflora species

Seed cryopreservation of Passiflora species

Ex situ Conservation and Cryopreservation of Orchid Germplasm

Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana

Contact Info

Product

Resources

About