Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization

Ji, Xiang‐Shan; Chen, S.L.; Tian, Yongsheng; Yu, Guo-Cai; Sha, Zongyao; Xu, Meng; Zhang, S.C.

doi:10.1016/j.aquaculture.2004.07.012

Cited by 37 publications

(19 citation statements)

References 19 publications

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“…DMSO had cryoprotective ability for sperm of some marine teleosts at concentrations of 5–20% (Jamieson and Leung 1991; Rideout et al 2003), while a 10% glycerol solution was less effective than DMSO and propylene glycol in the cryopreservation of winter flounder, Pseudopleuronectes americanus , spermatozoa (Rideout et al 2003). DMSO was more toxic than glycerol to cells and isolated cellular components (Arakawa et al 1990; Ji et al 2004). Sperm motility of rainbow trout, Oncorhynchus mykiss , was reduced at DMSO concentrations above 20% (Jamieson and Leung 1991).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Post‐thaw Membrane Integrity of Agulhas Sole, Austroglossus pectoralis, Spermatozoa Evaluated Using Flow Cytometry and Fluorescent Staining

Markovina

Kaiser

2009

J World Aquaculture Soc

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Section: Discussionmentioning

confidence: 99%

“…Temperature in the vials was not measured during these 6 min. Ji et al (2004) reported that equilibration at 6 cm above the liquid nitrogen surface for 10 min resulted in a linear temperature decrease from 16 to −15 C at rate of 31 C/min. The freezing rate from −12 to −180 C was 18.6 C/min.…”

Section: Discussionmentioning

confidence: 99%

Post‐thaw Membrane Integrity of Agulhas Sole, Austroglossus pectoralis, Spermatozoa Evaluated Using Flow Cytometry and Fluorescent Staining

Markovina

Kaiser

2009

J World Aquaculture Soc

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“…Thirty milliliter semen from 4-5 sea perch males were cryopreserved according to the method reported by Ji et al (2004) in November, 2006. After storage for about 2 years in liquid nitrogen, frozen sperm were thawed at 37°C waterbath and used for inducing gynogenesis in spotted halibut.…”

Section: Methodsmentioning

confidence: 99%

“…However, in some cases homologous sperm cannot be inactivated completely and contribute to paternal chromosomes to offspring. Nowadays, sperm of many fish species have been cryopreserved successfully (Ji et al 2004). Therefore, UV-irradiated heterologous frozen-thawed sperm can be used to activate eggs.…”

Section: Introductionmentioning

confidence: 99%

Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

Chen

Yang

et al. 2010

Aquacult Int

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In the present study, methodology of gynogenetic induction in spotted halibut were developed and optimized; the sex ratio of putative meiotic gynogenetic diploids was determined using AFLP-based molecular sexing technique; the homozygosity of gynogenetic population was assessed as opposed to cultivated population. The results showed that high percentage of meiotic gynogenetic diploids were generated when the eggs fertilized with irradiated heterologous sea perch frozen sperm (30-50 mJ cm -2 ) were cold shocked in sea water of -1°C for 40-75 min at 5 min after fertilization. About 15,200 diploid gynogenetic larvae were achieved and they exhibited normal morphology similar to diploid control. The gynogenetic diploids were 100% female, which first confirmed the female homogamete (XX/XY) sex determination in spotted halibut. The genetic analysis showed that the average H O was, respectively, 0.404 and 0.724 in gynogenetic population and cultivated population, indicating an increase of homozygosity in gynogenetic population.

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“…Samples from 5 to 8 individuals were pooled for further treatments. Following liquefaction at 37°C, motile spermatozoa were selected by densitygradient centrifugation (20 min at 600g) and afterwards the pellets were resuspended in 0.25 ml of extender medium (Ji et al 2004) for RNA extraction and the purity of the selected spermatozoa population was checked under a light microscope.…”

Section: Methodsmentioning

confidence: 99%

Transcript expression profiles of Takifugu rubripes spermatozoa and eggs by expressed sequence tag analysis

Shen

Cui

Gong

et al. 2008

Fish Physiol Biochem

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Two cDNA libraries from Takifugu rubripes spermatozoa and eggs were constructed and a total of 620 expressed sequence tag (EST) clones were generated from the two libraries: 300 clones are from the spermatozoa library and 320 clones are from the eggs library. The most abundant cDNA clones in the two libraries were identified. A total of 207 'contigs' (or single) EST clones were found to share significant sequence identity with known sequences in the GenBank database, representing at least 51 different genes. In order to understand the two types of germ cells further, the expression profiles of the identified clones in these cDNA libraries were analyzed. Furthermore, the presence of specific messenger RNAs in the spermatozoa and eggs has been demonstrated with BLAST analysis; the spermatozoa and egg library can supply unique and novel cDNA sequences in the Takifugu rubripes EST project. Another aim of this study is to identify cDNA clones that can be used as molecular markers for the analysis of the spermatogenesis and oogenesis in Takifugu rubripes. Six potential clones (S1-3 from spermatozoa and E1-3 from eggs) were selected to analyze their expression patterns by reverse transcription (RT)-PCR analyses. Half of these showed a specific expression in the expected tissue. Two of the clones were found by RT-PCR and in situ hybridization to be expressed specifically in the testis or ovary, and they maybe suitable molecular markers for the analysis of spermatogenesis and oogenesis.

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Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization

Cited by 37 publications

References 19 publications

Post‐thaw Membrane Integrity of Agulhas Sole, Austroglossus pectoralis, Spermatozoa Evaluated Using Flow Cytometry and Fluorescent Staining

Post‐thaw Membrane Integrity of Agulhas Sole, Austroglossus pectoralis, Spermatozoa Evaluated Using Flow Cytometry and Fluorescent Staining

Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus)

Transcript expression profiles of Takifugu rubripes spermatozoa and eggs by expressed sequence tag analysis

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