Cryopreservation of red snapper (Lutjanus argentimaculatus) sperm: Effect of cryoprotectants and cooling rates on sperm motility, sperm viability, and fertilization capacity

Vuthiphandchai, Verapong; Chomphuthawach, Sumate; Nimrat, Subuntith

doi:10.1016/j.theriogenology.2009.02.013

Cited by 39 publications

(32 citation statements)

References 49 publications

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“…The best result among the different ionic compositions and pH values tested in this study was achieved in the extender solution at 172 mOsm and pH 8.2. The extender HBSS with an osmolality of 200 mOsm was used for the cryopreservation of red snapper sperm and it was close to the values used in this study, but it was lower than the value of 315 mOsm used for mangrove red snapper sperm (Vuthiphandchai et al 2009). The cryopreservation of sperm has also been successful in other marine species using extender solutions with osmolalities close to 200 mOsm.…”

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Insupporting

confidence: 63%

“…In this study, a solution with 10% dMSO was the most efficient cryoprotectant, and a similar concentration was suggested for the cryopreservation of red snapper (Riley et al 2004) and mangrove red snapper sperm (Vuthiphandchai et al 2009). In other commercial marine species, satisfactory results were achieved with 10% dMSO for the cryopreservation of cobia Rachycentron canadum (Caylor et al 1994), common snook (Tiersch et al 2004) and fat snook sperm (Tiba et al 2009).…”

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Insupporting

confidence: 51%

“…When the sperm motility rate and motility time were considered, the best cooling rate for mutton snapper sperm was -60°C.min -1 . This value is higher than that used for the cryopreservation of sperm from red snapper (-16°C.min ; Vuthiphandchai et al 2009). Nevertheless, the cooling rate with the best result in this study is similar to the cooling rates used for the cryopreservation of grouper Epinephelus malabaricus (Chao et al 1992), kelp grouper Epinephelus moara (Miyaki et al 2005), fat snook (Tiba et al 2009) and red spotted grouper Epinephelus akaara sperm (He et al 2011).…”

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 67%

“…Therefore, to avoid controversy, a sperm:oocyte ratio of 200,000:1 was used in this study. The same ratio was used for cryopreserved sperm in mangrove red snapper (Vuthiphandchai et al 2009), although Riley et al (2004) used a ratio of 430,000:1 in the same species.…”

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 99%

“…In Brazil, the sperm cryopreservation has been used in few marine species, including dusky grouper Epinephelus marginatus (Sanches et al 2008), Brazilian flounder Paralichthys orbignyanus (Lanes et al 2008) and fat snook Centropomus parallelus (Tiba et al 2009 (Riley et al 2004, Vuthiphandchai et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sanches

Oliveira

Serralheiro

et al. 2013

An. Acad. Bras. Ciênc.

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This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders (pH 6.1; 7.8 and 8.2), two concentrations of dimethyl sulfoxide (dMSO, 5 and 10%) and three cooling rates (-90; -60 and -30°C.min ) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes (1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time (P < 0.05) was achieved by combining extender C (ph 8.2) with dMSO (10%) and cooling rate of -60°C.min -1 (P < 0.05). The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.

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Section: -1 (Vuthiphandchai Et Al 2009) the Variation Insupporting

confidence: 63%

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Insupporting

confidence: 51%

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 67%

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sanches

Oliveira

Serralheiro

et al. 2013

An. Acad. Bras. Ciênc.

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Cryopreservation of Marine Fish Sperm

Tian

Zhang

et al. 2020

Cryopreservation of Fish Gametes

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Development of sperm vitrification protocols for two endangered salmonid species: the Adriatic grayling, Thymallus thymallus, and the marble trout, Salmo marmoratus

Kása

Lujić

Marinović

et al. 2018

Fish Physiol Biochem

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Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA). Fertilization trials were carried out to test the effectiveness of vitrification in the case of grayling. A protocol utilizing a glucose-based extender, 30% cryoprotectants (15% methanol + 15% propylene glycol), 1:1 dilution ratio, and droplets of 2 μl on a Cryotop as cooling device yielded the highest post-thaw motility values for both Adriatic grayling (7.5 ± 6.5%) and marble trout (26.6 ± 15.8%). Viable embryos were produced by fertilizing eggs with vitrified grayling sperm (hatching 13.1 ± 11.7%, control hatching 73.9 ± 10.4%). The vitrification protocol developed in this study can be utilized in the conservation efforts for the two species as an alternative to slow-rate freezing when working in field conditions or when specific equipment necessary for slow-rate freezing is not available.

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Cryopreservation of red snapper (Lutjanus argentimaculatus) sperm: Effect of cryoprotectants and cooling rates on sperm motility, sperm viability, and fertilization capacity

Cited by 39 publications

References 49 publications

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Cryopreservation of Marine Fish Sperm

Development of sperm vitrification protocols for two endangered salmonid species: the Adriatic grayling, Thymallus thymallus, and the marble trout, Salmo marmoratus

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