Cryopreservation of red blood cells: Effect on rheologic properties and associated metabolic and nitric oxide related parameters

Bizjak, Daniel Alexander; Jungen, Pia; Bloch, Wilhelm; Grau, Marijke

doi:10.1016/j.cryobiol.2018.08.001

“…We can thus speculate that the RBC shape alterations observed in the high dose groups of this investigation may be caused by the glycerol permeating cell membrane following a concentration gradient (32) generated by the signi cant rise in glycerol circulating concentrations consequent to the dietary administration. Shape alterations of sheep RBCs reported in this trial are similar to those described in human glycerolized RBCs (34,45), and might thus be associated with erythrocyte impaired functionality. In addition, when the average size of RBC's increases, they can fail to pass the capillaries in the microcirculation and be removed from the circulation (48).…”

Section: Discussionsupporting

confidence: 73%

“…It has been reported that glycerol permeation in the RBC membrane resulted in shape alterations (increased MCV and RDW-SD) which appeared to be closely related to membrane alterations, increased permeability to ions and increased osmotic fragility (45). These results are con rmed by another study in which the glycerol-dependent shape alterations (increased MCV and decreased MCHC) were also associated with an electrolyte shift (34). Glycerol is indeed a polar molecule which is known to alter the ionic strength and the dielectric constant of aqueous solutions (46).…”

Section: Discussionmentioning

confidence: 72%

Administration of Glycerol-Based Formulations in Sheep Results in Similar Ovulation Rate to eCG but Red Blood Cell Indices May Be Affected

Porcu

¹

,

Sotgiu

²

,

Pasciu

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Background: The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe’s ovarian activity during an induced follicular phase and compared to the effects of a hormonal treatment with equine chorionic gonadotrophin (eCG). Results: Glycerol was administered alone (G groups: 90% glycerol and 10% water; % v/v) or in combination with propylene glycol (M groups: 70% glycerol, 20% propylene glycol, 10% water; % v/v). Treatments were formulated to provide 100%, 75%, 50% and 25% of the amount of energy supplied in previous experiments. Obtained results showed that the formulations G75 and M75 (22.5% and 18.2% on DM basis, respectively) induce metabolic changes comparable to those induced by M100. The latter dose has been already evaluated for its effects on sheep metabolism and reproductive performance. However, with these high doses, plasma osmolality increased significantly, and RBC indices showed significant alterations. The low dose groups (G25 and M25, 8.6% and 6.9% on DM basis, respectively) did not show any alterations in plasma osmolality and RBC indices, but the metabolic milieu differed markedly from that of M100. Between the medium dose groups, M50 (12.9% on DM basis) showed a more comparable milieu to M100 than G50 (15.9% on DM basis) and no RBC alterations. Therefore, M75, G75 and M50 doses were tested for their effect on ovarian functions and proved to be equally effective as eCG. Conclusion: The results of the present study evidenced an alteration of RBC indices, and possibly of their functions, as a side effect of glycerol administration at high doses in the diet of ewes. Therefore, protocols foreseeing the administration of glycerol should be tested for their effects on RBC indices and functions. In general terms, the medium dose of the glucogenic mixture (12.9% of dietary DM on offer) should be preferred.

show abstract

“…These results are confirmed by another study in which the glycerol-dependent shape alterations (increased MCV and decreased MCHC) were also associated with an electrolyte shift (34). Glycerol is indeed a polar molecule which is known to alter the ionic strength and the dielectric constant of aqueous solutions (46).…”

Section: Discussionsupporting

confidence: 65%

“…RBCs (34,45), and might thus be associated with erythrocyte impaired functionality. In addition, when the average size of RBC's increases, they can fail to pass the capillaries in the microcirculation and be removed from the circulation (48).…”

Section: Discussionmentioning

confidence: 99%

Glycerol-based formulations increases ovulation rate in sheep similarly to eCG but red blood cell indices may be affected

Porcu¹,

Sotgiu²,

Pasciu³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Background: The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe’s ovarian activity during an induced follicular phase and compared to the effects of a hormonal treatment with equine chorionic gonadotrophin (eCG). Results: Glycerol was administered alone (G groups: 90% glycerol and 10% water; % v/v) or in combination with propylene glycol (M groups: 70% glycerol, 20% propylene glycol, 10% water; % v/v). Treatments were formulated to provide 100%, 75%, 50% and 25% of the amount of energy supplied in previous experiments. Obtained results showed that the formulations G75 and M75 (22.5% and 18.2% on DM basis, respectively) induce metabolic changes comparable to those induced by M100. The latter dose has been already evaluated for its effects on sheep metabolism and reproductive performance. However, with these high doses, plasma osmolality increased significantly, and RBC indices showed significant alterations. The low dose groups (G25 and M25, 8.6% and 6.9% on DM basis, respectively) did not show any alterations in plasma osmolality and RBC indices, but the metabolic milieu differed markedly from that of M100. Between the medium dose groups, M50 (12.9% on DM basis) showed a more comparable milieu to M100 than G50 (15.9% on DM basis) and no RBC alterations. Therefore, M75, G75 and M50 doses were tested for their effect on ovarian functions and proved to be equally effective as eCG. Conclusion: The results of the present study evidenced an alteration of RBC indices, and possibly of their functions, as a side effect of glycerol administration at high doses in the diet of ewes. Therefore, protocols foreseeing the administration of glycerol should be tested for their effects on RBC indices and functions. In general terms, the medium dose of the glucogenic mixture (12.9% of dietary DM on offer) should be preferred.

show abstract

“…Максимально длительное сохранение крови как наиболее ценного биологического ресурса является одной из основных задач современной гематологии и трансфузиологии [1]. При этом наиболее значимым критерием адекватности консервации служит сохранность ее физиологических характеристик и физико-химических свойств [1][2][3][4]. Распространенным вариантом методики длительного хранения крови является ее нахождение в условиях низких (околонулевых) и очень низких (-80 °С; -196 °С и др.)…”

Section: Introductionunclassified

Experimental Evaluation of Cryoprotector Systemic Effects

Мартусевич

¹

,

Костяев

²

,

Kovaleva

³

2019

Kuban. nauсh. med. vestnik

0

View full text Add to dashboard Cite

Aim. Experimental evaluation of the effect of some cryoprotectors on crystallogenic properties of mice blood serum at intraperitoneal administration.Material and methods. The experiment was performed on 40 adult white line mice divided into 4 equal groups. Animals from the control (fi rst) group received a single intraperitoneal injection of saline (0.5 ml agent/animal), animals of the second-fourth groups — 3 % solution of glycerin, dimethyl sulphoxide and trombocryodmac (N, N-dimethylacetamide), respectively, in the same quantity.Results. It is shown that single intraperitoneal introduction of cryoprotectants (solutions of glycerin, dimethyl sulphoxide and trombocryodmac) has a systemic effect on the mouse organism, which is manifested through the changes of crystallogenic properties of blood serum. 3% solution of glycerin, dimethyl sulphoxide and trombocryodmac have multidirectional infl uence on crystallogenic properties of mouse blood serum. The most pronounced physiological effect was established for dimethyl sulphoxide.Conclusion. Our studies demonstrate the presence of systemic effects in cryopreservative substances. It was confi rmed by the changes of crystallogenic properties of mice blood serum under intraperitoneal administration of cryopreservatives.

show abstract

Cryopreservation of red blood cells: Effect on rheologic properties and associated metabolic and nitric oxide related parameters

Cited by 18 publications

References 47 publications

Administration of Glycerol-Based Formulations in Sheep Results in Similar Ovulation Rate to eCG but Red Blood Cell Indices May Be Affected

Administration of Glycerol-Based Formulations in Sheep Results in Similar Ovulation Rate to eCG but Red Blood Cell Indices May Be Affected

Glycerol-based formulations increases ovulation rate in sheep similarly to eCG but red blood cell indices may be affected

Experimental Evaluation of Cryoprotector Systemic Effects

Contact Info

Product

Resources

About