Cryopreservation of rat MSCs by use of a programmed freezer with magnetic field

Kojima, Sunao; Kaku, Masato; Kawata, Toshitsugu; Sumi, Hiromi; Shikata, Hanaka; Abonti, Tahsin Raquib; Kojima, Sekizo; Fujita, Tadashi; Motokawa, Masaharu; Tanne, Kazuo

doi:10.1016/j.cryobiol.2013.08.003

Cited by 25 publications

(20 citation statements)

References 18 publications

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“…[113]. The cells were then kept at -150 plunge temperature was -30 °C [113]. If the plunge temperature is higher than the optimal 664 temperature, cells are damaged by intracellular ice formation but if the plunge temperature is too (Table 4).…”

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confidence: 99%

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Mesenchymal stromal cells derived from various tissues: Biological, clinical and cryopreservation aspects

et al. 2015

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Originally isolated from bone marrow, mesenchymal stromal cells (MSCs) have since been obtained from various fetal and post-natal tissues and are the focus of an increasing number of clinical trials. Because of their tremendous potential for cellular therapy, regenerative medicine and tissue engineering, it is desirable to cryopreserve and bank MSCs to increase their access and availability. A remarkable amount of research and resources have been expended towards optimizing the protocols, freezing media composition, cooling devices and storage containers, as well as developing good manufacturing practices in order to ensure that MSCs retain their therapeutic characteristics following cryopreservation and that they are safe for clinical use. Here, we first present an overview of the identification of MSCs, their tissue sources and the properties that render them suitable as a cellular therapeutic. Next, we discuss the responses of cells during freezing and focus on the traditional and novel approaches used to cryopreserve MSCs. We conclude that viable MSCs from diverse tissues can be recovered after cryopreservation using a variety of freezing protocols, cryoprotectants, storage periods and temperatures. However, alterations in certain functions of MSCs following cryopreservation warrant future investigations on the recovery of cells post-thaw followed by expansion of functional cells in order to achieve their full therapeutic potential.

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confidence: 99%

“…and differentiation to mesenchymal-specific lineages [146]. [113]. The cells were then kept at -150 plunge temperature was -30 °C [113].…”

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confidence: 99%

Mesenchymal stromal cells derived from various tissues: Biological, clinical and cryopreservation aspects

et al. 2015

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“…For MSCs cryopreservation, we showed that the number of viable MSCs after cryopreservation by CAS freezer was higher than for those cryopreserved by non-magnetic field freezer, and we proved that MSCs cryopreserved by CAS freezer can differentiate to osteoblasts and adipocytes [13]. However, the proliferation rate in the CAS group was still significantly lower than for non-cryopreserved MSCs [13]; it was necessary to improve the CAS system for MSCs cryopreservation.…”

Section: Discussionmentioning

confidence: 82%

“…These clusters will injure cell membranes and as a result, cells will be damaged. On the other hand, alternating magnetic fields with an induced electric field vibrate cells and water molecules by a non-thermal mechanism [13,14]. These vibrations were amplified in synchronicity with mechanical and thermal vibration (CAS vibration), which can prevent intracellular ice crystal formation.…”

Section: Discussionmentioning

confidence: 98%

“…Furthermore, positive reaction of Oil Red O staining and high amounts of PPARg and FABP4 mRNAs were shown in CAS freezer groups after adipogenic differentiation. These findings suggested that a CAS freezer can maintain high survival and proliferation rates of MSCs and maintain both adipogenic and osteogenic differentiation abilities [13]. However, proliferation rates of MSCs in the CAS group was still significantly less than that of non-cryopreserved MSCs.…”

Section: Introductionmentioning

confidence: 89%

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