Cryopreservation of mouse 2-cell embryos and ova by vitrification: methodologie studies

Friedler, Shevach; Shen, Eve; Lamb, Emmet J.

doi:10.1016/s0015-0282(16)59361-3

Cited by 23 publications

(14 citation statements)

References 19 publications

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“…Both events may compromise survival due to membrane and cell injuries or cryoprotectant toxicity. Such effects are not prominent for early stages, since the relative efficacy observed in this study for the compact morula stage was high and fell well within values (81 to 89%) reported by others [1,5,15,22] following the vitrification of mouse embryos at different stages of development under dis-…”

Section: Discussionsupporting

confidence: 91%

In vitro and in vivo survival of mouse morulas and blastocysts following vitrification in 45% glycerol

Bertolini

Lange

Rodrigues

2018

Acta Scientiae. Vet.

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Section: Discussionsupporting

confidence: 91%

In vitro and in vivo survival of mouse morulas and blastocysts following vitrification in 45% glycerol

Bertolini

Lange

Rodrigues

2018

Acta Scientiae. Vet.

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“…The present study examined only two methods of cryopreservation for the oocyte, vitrification with DMSO and sucrose, and slow cooling with 1,2 propanediol and sucrose. Both of these procedures have been shown to be highly successful for cleavage-stage mouse (Friedler et al, 1987;Rall and Fahy, 1985;Wilson and Quinn, 1989) and human (Mandelbaum et al, 1988;Testart et al, 1986) embryos. In general, our findings for the immature mouse oocyte demonstrate that while profound changes occur at the nuclear and cellular levels, normal cytoplasmic structure and organization are progressively restored after thawing and culture, and that meiosis resumes and progresses in an apparently normal manner.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic, cellular, and developmental consequences of cryopreservation of immature and mature mouse and human oocytes

Blerkom

Davis

1994

Microscopy Res & Technique

154

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This study examined the effects of cryopreservation on cellular organization, chromosomal complement, and developmental potential of immature and mature mouse and human oocytes. Chromosomal analyses were performed by DNA fluorescence microscopy and karyotyping on the same metaphase II-stage oocytes before and after freezing. Cellular analyses involved electron microscopy, time-lapse video recording, and fluorescent-probe microscopy of cortical granules. The findings demonstrate that while profound cytoplasmic, nuclear, and nucleolar alterations occur in the immature oocyte during cryopreservation, an apparently normal nucleus and cytoplasm is re-established progressively after thawing and culture. The resulting oocytes mature at high frequency and for the mouse, are fertilizable and capable of normal preimplantation of embryogenesis. Cryopreservation of mature mouse and human oocytes is not accompanied by a significant increase in the frequency of aneuploidy. However, cryopreserved human oocytes, while fertilizable, arrest development during the early cleavage stages and display aberrant patterns of cytokinesis. The possible etiologies of developmental failure in the human embryo that may be related to oocyte cryopreservation, as well as the potential benefits of cryopreservation of the immature oocyte, are discussed with respect to clinical and commercial applications.

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“…Propanediol has been used successfully as a cryoprotectant for fertilized, pronuclearstage oocytes [Testart et a]., 19861, but has not been widely used, either alone or in cryoprotectant mixtures, to cryopreserve unfertilized oocytes [Critser et al, 1986;Al-Hasani et al, 1987;Friedler et al, 1987Friedler et al, , 1988KO and Threlfall, 19881. Although those studies did not report any parthenogenetic activation, it may have been overlooked since they do not mention control groups to test for parthenogenetic activation.…”

Section: Discussionmentioning

confidence: 99%

Parthenogenetic activation of unfertilized mouse oocytes by exposure to 1,2‐propanediol is influenced by temperature, oocyte age, and cumulus removal

Shaw¹,

Trounson²

1989

Gamete Research

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Cumulus-intact and -denuded unfertilized oocytes from two mouse strains were exposed to 1.5 M ethanol (EtOH) or two cryoprotectant solutions, 1.5 M propanediol (PROH) or 1.5 M dimethylsulfoxide (DMSO), for 4.5 min at 27 degrees C, and the proportion of activating or degenerating oocytes studied. Exposure to DMSO did not significantly increase activation above that of oocytes not exposed to DMSO. Treatment of oocytes in PROH resulted in the activation of up to 87% of viable oocytes. This was significantly higher (P less than .01) than in control oocytes and comparable to the rate of activation after treatment with EtOH (59-96% activation). In solutions at 1 degree C, 47% of control oocytes were activated, which was not significantly different from the rate of activation in EtOH (36%) or PROH (50%) at 1 degree C. Following treatment with PROH, up to 87% of oocytes degenerated within a period of 6 h in vitro. The age of the oocytes (h post hCG) and the time of cumulus removal with the enzyme hyaluronidase, relative to the time of exposure to the chemicals, influenced the level of degeneration in most groups. Significantly fewer oocytes degenerated when cumulus cells were removed before treatment (0-31%) than when the cumulus was left intact throughout the treatment and 6 h culture period (10-87%). Exposure to PROH at 1 degree C reduced oocyte degeneration to 5%. We conclude that PROH causes significantly greater losses of oocytes as a result of parthenogenetic activation and degeneration than of exposure to DMSO.

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Cryopreservation of mouse 2-cell embryos and ova by vitrification: methodologie studies

Cited by 23 publications

References 19 publications

In vitro and in vivo survival of mouse morulas and blastocysts following vitrification in 45% glycerol

In vitro and in vivo survival of mouse morulas and blastocysts following vitrification in 45% glycerol

Cytogenetic, cellular, and developmental consequences of cryopreservation of immature and mature mouse and human oocytes

Parthenogenetic activation of unfertilized mouse oocytes by exposure to 1,2‐propanediol is influenced by temperature, oocyte age, and cumulus removal

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