Cryopreservation of microencapsulated murine mesenchymal stem cells genetically engineered to secrete erythropoietin

Gurruchaga, Haritz; Ciriza, Jesús; Burgo, Laura Sáenz del; Rodríguez-Madoz, Juan R.; Santos, Edorta; Prósper, Felipe; Hernández, Rosa Marı́a; Orive, Gorka

doi:10.1016/j.ijpharm.2015.02.047

Cited by 33 publications

(33 citation statements)

References 44 publications

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“…Permeating CPAs such as glycerol, are molecules of low molecular weight that can go through the cell membrane by osmosis, replace the water inside the cells and hold the non-freezable water within the cells [17]. The most common is dimethylsulfoxide (DMSO), it is included in the range of 5 to 12% (v/v) in most of the CPA solutions for every cell type with excellent results [8,11,12,14,15]. For example Hemacord®, an allogeneic cord blood hematopoietic progenitor cell therapy product which is approved by the FDA, is cryopreserved with DMSO obtaining great results in clinics.…”

Section: 1choice Of Cpa Solutionmentioning

confidence: 99%

“…The storage in vapor or liquid nitrogen (-160°C to -196°C) of cell-based products is the most often used method to preserve slowly cooled samples [8,13,14,29,35,64]. The temperatures achieved are far below of the Tg temperatures assuring safe long-term preservation of cell-based products.…”

Section: 1choice Of Cpa Solutionmentioning

confidence: 99%

“…In this sense, as processes occurring throughout warming of slowly cooled samples are not easy to predict, fast thawing has been favored in order to prevent the feared ice crystal growth. The most used way to thaw samples is by their immersion in a water bath at 37°C obtaining good results over decades [13,14,35,36,38,64]. However, as sample contact with water could produce whether a bioartificial liver device could be preserved [79].…”

Section: Thawingmentioning

confidence: 99%

“…This method is also used for larger samples and technically is a simpler procedure than vitrification [4]. Moreover, slow freezing does not need advanced equipment, and in the case of alginate cell-based products, almost all samples are preserved using this protocol [12][13][14][15]. This review will discuss the slow freezing method for the preservation of cell-based products.…”

Section: Introductionmentioning

confidence: 99%

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Advances in the slow freezing cryopreservation of microencapsulated cells

Gurruchaga

Burgo

Hernández

et al. 2018

Journal of Controlled Release

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Over the past few decades, the use of cell microencapsulation technology has been promoted for a wide range of applications as sustained drug delivery systems or as cells containing biosystems for regenerative medicine. However, difficulty in their preservation and storage has limited their availability to healthcare centers. Because the preservation in cryogenic temperatures poses many biological and biophysical challenges and that the technology has not been well understood, the slow cooling cryopreservation, which is the most used technique worldwide, has not given full measure of its full potential application yet. This review will discuss the different steps that should be understood and taken into account to preserve microencapsulated cells by slow freezing in a successful and simple manner. Moreover, it will review the slow freezing preservation of alginate-based microencapsulated cells and discuss some recommendations that the research community may pursue to optimize the preservation of microencapsulated cells, enabling the therapy translate from bench to the clinic.

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Section: 1choice Of Cpa Solutionmentioning

confidence: 99%

Section: 1choice Of Cpa Solutionmentioning

confidence: 99%

Section: Thawingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Advances in the slow freezing cryopreservation of microencapsulated cells

Gurruchaga

Burgo

Hernández

et al. 2018

Journal of Controlled Release

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“…A three-step slow cooling process [123] with induced ice nucleation using 10% DMSO (also suggested elsewhere [124]) has been shown to maximize cell viability, and the cells retain their metabolic and differentiation capacity. Cell encapsulation may also be beneficial for short-term storage, such as for transport [125].…”

Section: Formulation and Storage Of Stem Cellsmentioning

confidence: 99%

The Challenge of Human Mesenchymal Stromal Cell Expansion: Current and Prospective Answers

Elseberg¹,

Leber²,

Weidner³

et al. 2017

New Insights Into Cell Culture Technology

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In the field of cell therapy, allogenic human mesenchymal stromal cells (hMSCs) are often used in clinical trials, creating a demand for cell mass production using efficient dynamic bioreactor systems. As an advanced therapy medicinal product (ATMP), such cells should meet certain special requirements, including product specifications requiring a production process compatible with good manufacturing practice (GMP). The development of processes in which the cells are the product therefore remains a significant challenge. This chapter describes the requirements at different steps in the upstream and downstream phases of such dynamic processes. Potential solutions are presented and future prospects are discussed, including the selection of media and carriers for the strictly adherent growing cells, allowing efficient cell adhesion and detachment. Strategies for dynamic cultivation in bioreactors are described in detail for fixed-bed and stirred-tank reactors based on GMP requirements and the integration of process analytical technology (PAT). Following cell harvest, separation and purification, the formulation and storage of the product are also described. Finally, the chapter covers important cell quality characteristics necessary for the approval of ATMPs.

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Chondroitin and Dermatan Sulfate Bioinks for 3D Bioprinting and Cartilage Regeneration

Lafuente‐Merchan

Ruiz‐Alonso

Zabala

et al. 2022

Macromolecular Bioscience

Self Cite

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Cartilage is a connective tissue which a limited capacity for healing and repairing. In this context, osteoarthritis (OA) disease may be developed with high prevalence in which the use of scaffolds may be a promising treatment. In addition, three‐dimensional (3D) bioprinting has become an emerging additive manufacturing technology because of its rapid prototyping capacity and the possibility of creating complex structures. This study is focused on the development of nanocellulose‐alginate (NC‐Alg) based bioinks for 3D bioprinting for cartilage regeneration to which it is added chondroitin sulfate (CS) and dermatan sulfate (DS). First, rheological properties are evaluated. Then, sterilization effect, biocompatibility, and printability on developed NC‐Alg‐CS and NC‐Alg‐DS inks are evaluated. Subsequently, printed scaffolds are characterized. Finally, NC‐Alg‐CS and NC‐Alg‐DS inks are loaded with murine D1‐MSCs‐EPO and cell viability and functionality, as well as the chondrogenic differentiation ability are assessed. Results show that the addition of both CS and DS to the NC‐Alg ink improves its characteristics in terms of rheology and cell viability and functionality. Moreover, differentiation to cartilage is promoted on NC‐Alg‐CS and NC‐Alg‐DS scaffolds. Therefore, the utilization of MSCs containing NC‐Alg‐CS and NC‐Alg‐DS scaffolds may become a feasible tissue engineering approach for cartilage regeneration.

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Cryopreservation of microencapsulated murine mesenchymal stem cells genetically engineered to secrete erythropoietin

Cited by 33 publications

References 44 publications

Advances in the slow freezing cryopreservation of microencapsulated cells

Advances in the slow freezing cryopreservation of microencapsulated cells

The Challenge of Human Mesenchymal Stromal Cell Expansion: Current and Prospective Answers

Chondroitin and Dermatan Sulfate Bioinks for 3D Bioprinting and Cartilage Regeneration

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