Cryopreservation of In Vitro Tissues of Deciduous Forest Trees

Häggman, Hely; Rusanen, Mari; Jokipii, S. G.

doi:10.1007/978-0-387-72276-4_14

Cited by 19 publications

(10 citation statements)

References 36 publications

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“…The different conservation strategies for forest biodiversity are well defined (Häggman et al 2008) and, among these, cryopreservation may be considered as a complementary strategy for storage of plant cells, tissues, seeds and embryos. Cryopreservation is normally viewed as a secondary storage method designed as a secure backup to living collections (Reed 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, production of transgenic tree species and molecular breeding procedures require functional cryopreservation protocols (Häggman et al 2001). Cryopreservation is increasingly applied on a large-scale basis to woody plants (Reed et al 1998;Häggman et al 2008), and as regards forest species, it is most commonly applied to conifers. In the commercial exploitation of conifers, this technique is now routinely applied to embryogenic lines awaiting field testing results (Park et al 1998;Sutton 2002).…”

Section: Introductionmentioning

confidence: 99%

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Establishment of cryopreserved gene banks of European chestnut and cork oak

Vidal

Viéitez

Fernández³

et al. 2010

Eur J Forest Res

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Cryopreservation of selected genotypes of European chestnut and cork oak was carried out in two laboratories in a project involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (cork oak). The cryopreservation technique comprised of vitrification of shoot apices isolated from in vitro stock shoot cultures (chestnut) and somatic embryos (cork oak). Forty-three out of 46 chestnut genotypes assayed survived the freezing process, but only 63% recovered their capacity to produce new shoots. After completion of multiplication and rooting steps, the surviving shoots produced plants that were morphologically identical to those derived from non-supercooled material. All 51 cork oak genotypes withstood freezing and were able to produce new somatic embryos through a process of secondary embryogenesis. Multiplication and germination of the recovered embryos enabled production of plants that were morphologically identical to those derived from nonsupercooled material. In light of the results obtained, longterm cryopreservation of these species is feasible, thereby ensuring conservation of valuable genotypes during field evaluation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Establishment of cryopreserved gene banks of European chestnut and cork oak

Vidal

Viéitez

Fernández³

et al. 2010

Eur J Forest Res

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“…The potential somaclonal variation of in vitro tissue cultures as well as the mutagenic potential of the most popular cryoprotectant component of the PVS2 vitrification solution, DMSO, are well known, although to date there is no evidence of morphological or genetic alterations in forest trees as a result of cryopreservation (Häggman et al 2008). Aronen et al (1999) reported genetic changes in DMSOtreated, non-supercooled samples, but not in DMSO-treated and cryostored samples of Abies cephalonica somatic embryos during cryopreservation.…”

Section: Cryopreservationmentioning

confidence: 97%

“…In general, strategies for forest biodiversity conservation are today well defined (Häggman et al 2008), among which cryopreservation is viewed as a complementary, secondary storage method designed as a secure backup for living collections (Reed 2008). Well-defined in vitro techniques are required to recover the multiplication capacity of supercooled material via axillary shoot growth or somatic embryogenesis.…”

Section: Cryopreservationmentioning

confidence: 99%

Application of biotechnological tools to Quercus improvement

et al. 2011

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The genus Quercus, which belongs to the family Fagaceae, is native to the northern hemisphere and includes deciduous and evergreen species. The trees of the different species are very important from both economic and ecological perspectives. Application of new technological approaches (which span the fields of plant developmental biology, genetic transformation, conservation of elite germplasm and discovery of genes associated with complex multigenic traits) to these long-rotation hardwoods may be of interest for accelerating tree improvement programs. This review provides a summary of the advances made in the application of biotechnological tools to specific oak species. Significant progress has been made in the area of clonal propagation via organogenesis and somatic embryogenesis (SE). Standardized procedures have been developed for micropropagating the most important European (Q. robur, Q. petarea, Q. suber) and American (Q. alba, Q. bicolor, Q. rubra) oaks by axillary shoot growth. Although regenerated plantlets are grown in experimental trials, large-scale propagation of oak species has not been carried out. The induction of SE in oaks from juvenile explants is generally not problematic, although the use of explants other than zygotic embryos is much less efficient. During the last decade, enormous advances have been made in inducing SE from selected adult trees, mainly specimens of pedunculate oak (Q. robur) and cork oak (Q. suber). Advances in the understanding of the maturation and germination steps are required for better use of embryogenic process in clonal forestry. Quercus species are late-maturing and late-flowering, exhibit irregular seed set, and produce seeds that are recalcitrant to storage by conventional procedures. Vitrification-based cryopreservation techniques were used successfully in somatic embryos of pedunculate oak and cork oak, and an applied genbank of cork oak selected genotypes is now under development. The feasibility of genetic transformation of pedunculate oak and cork oak somatic embryos by means of co-culture techniques with several strains of Agrobacterium tumefaciens has also been demonstrated. To date, most research on the genomics of Quercus species has concerned population genetics. Approaches using functional genomics to examine the molecular and cellular mechanisms that control organogenesis and or somatic embryogenesis are still scarce, and efforts on the isolation and characterization of genes related to other specific traits should be intensified in the near future, as this would help improve the practical application of clonal forestry in recalcitrant species such as oaks.

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“…Harding and Benson (2001) used SSRs to examine Solanum tuberosum L. and found no differences between cryopreserved and control shoots. Haggman et al (2008) noted that currently, there is no evidence of cryopreservationinduced genetic or morphological changes in forest trees.…”

Section: Introductionmentioning

confidence: 94%

Genetic stability of cryopreserved shoot tips of Rubus germplasm

Castillo

Bassil

Wada

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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Questions often arise concerning the genetic stability of plant materials stored in liquid nitrogen for long time periods. This study examined the genetic stability of cryopreserved shoot tips of Rubus germplasm that were stored in liquid nitrogen for more than 12 yr, then rewarmed and regrown. We analyzed the genetic stability of Rubus grabowskii, two blackberry cultivars ("Hillemeyer" and 'Silvan'), and one raspberry cultivar ("Mandarin") as in vitro shoots and as field-grown plants. No morphological differences were observed in greenhouse-grown cryopreserved plants when compared to the control mother plants. In the field, cryopreserved plants appeared similar but were more vigorous than mother plants, with larger leaves, fruit, and seeds. Single sequence repeats (SSR) and amplified fragment length polymorphism (AFLP) analyses were performed on shoots immediately after recovery from cryopreservation and on shoots subcultured for 7 mo before analysis. Ten SSR primers developed from "Marion" and "Meeker" microsatellite-enriched libraries amplified one to 15 alleles per locus, with an average of seven alleles and a total of 70 alleles in the four genotypes tested. No SSR polymorphisms were observed between cryopreserved shoots and the corresponding mother plants regardless of subculture. Although no polymorphisms were detected in shoots analyzed immediately after recovery from cryopreservation, AFLP polymorphisms were detected in three of the four Rubus genotypes after they were subcultured for 7 mo. Field-grown plants from the polymorphic shoot tips of R. grabowskii and 'Silvan' displayed the same AFLP fingerprints as their corresponding mother plants. Only longcultured in vitro shoot tips displayed polymorphisms in vitro, and they were no longer detected when the plants were grown ex vitro. The transitory nature of these polymorphisms should be carefully considered when monitoring for genetic stability.

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Cryopreservation of In Vitro Tissues of Deciduous Forest Trees

Cited by 19 publications

References 36 publications

Establishment of cryopreserved gene banks of European chestnut and cork oak

Establishment of cryopreserved gene banks of European chestnut and cork oak

Application of biotechnological tools to Quercus improvement

Genetic stability of cryopreserved shoot tips of Rubus germplasm

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