Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity

Donnelly, Eilish; McClure, Neil; Lewis, Sheena

doi:10.1016/s0015-0282(01)02834-5

Cited by 212 publications

(188 citation statements)

References 25 publications

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“…The effects of cryopreservation on the integrity of the sperm nucleus are not well characterized. It is reported that the cryopreservation process of freezing and thawing can increase inappropriate chromatin condensation in human (Royere et al 1988, Royere et al 1991, Donnelly et al 2001, Hammadeh et al 2001, boar (Fraser & Strzezek 2004), horse (Linfor & Meyers 2002), and ram (Peris at al. 2004) sperm.…”

Section: Effects Of Cryopreservation On Sperm Qualitymentioning

confidence: 99%

“…A significant correlation between the presence of nuclear DNA alterations in mature spermatozoa and poor sperm parameters or impaired reproductive efficiency is reported in both humans and animals (Ron-el et al 1991, Hughes et al 1996, Edwards & Beard 1999. To date, studies show that the cryopreservation process causes DNA damage to mammalian sperm in human (Royere et al 1988, 1991, Donnelly et al 2001, Hammadeh et al 2001, boar (Fraser & Strzezek 2004), and ram (Peris et al 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse

Yıldız¹,

Ottaviani²,

Law³

et al. 2007

Reproduction

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Efficient freezing, archiving, and thawing of sperm are essential techniques to support large scale research programs using mouse models of human disease. The purpose of this study was to investigate the effects of variable combinations and concentrations of cryoprotectants on sperm-assessment parameters of frozen-thawed mouse sperm in order to optimize cryopreservation protocols. Sperm was frozen using combinations of 3% skim milkC0.2 or 0.3 M nonpermeating raffinose with either permeating glucose, fructose, propylene glycol, ethylene glycol, glycerol, or sodium pyruvate in CD-1, C3FeB6F1/J, B6129SF1, C57BL/6NCrIBR, 129S/SvPaslco, and DBA/2NCrIBR mice. Sperm-assessment parameters included progressive motility, plasma membrane integrity (SYBR-14CPI), in vitro fertilization rate, and in vitro embryo development rate to blastocyst. DNA content analysis of sperm was measured by the sperm chromatin structure assay (SCSA). 0.3 M raffinose with 0.1 M fructose significantly improved post-thaw sperm-assessment parameters for CD-1, C3B6F1, B6129SF1 mice (P!0.05-0.01), whereas 0.2 M raffinose with 0.1 M glycerol or 0.1 M fructose enhanced sperm assessment values for C57BL/6 and 129S mice (P!0.01), compared to 0.3 M raffinose alone. DNA fragmentation during cryopreservation was significantly increased in all strains evaluated when compared with fresh control sperm in a strain-dependent manner (P!0.01). Supplementation with permeating glycerol or fructose to the cryoprotectant (CPA) solution showed a significant protective effect to DNA integrity when cryopreserving sperm from C57BL/6 and 129S mice. Damage to sperm DNA significantly decreased the rate of in vitro embryo development to blastocyst in C57BL/6 mice. The type of monosaccharide sugar or polyols, CPA molarity, and combination of permeating and nonpermeating cryoprotectant are significant factors for improving progressive motility, plasma membrane integrity, DNA integrity, in vitro fertilization rate, and in vitro embryo development rate to blastocyst in cryopreserved mouse sperm. Reproduction (2007) 133 585-595

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Section: Effects Of Cryopreservation On Sperm Qualitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse

Yıldız¹,

Ottaviani²,

Law³

et al. 2007

Reproduction

View full text Add to dashboard Cite

show abstract

“…To date, no sensitive highly quantitative method has been used to examine the relationship between subject's age and sperm DNA damage. Methods such as filter elution assays (19 -21), terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) (22), comet assay, or microgel electrophoresis technique (23)(24)(25)(26)(27)(28)(29)(30)(31)(32) have been used to quantify DNA damage in sperm cells. The neutral microgel electrophoresis technique is a sensitive and simple method of evaluating double-strand break DNA damage in individual sperm cells (24,(33)(34)(35).…”

mentioning

confidence: 99%

Effects of age on DNA double-strand breaks and apoptosis in human sperm

Singh

Müller

Berger

2003

Fertility and Sterility

395

223

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“…Cryopreservation leads to increases in chromatin instability and single-strand DNA fragmentation [8][9][10]. Although not investigated in the current analysis, the recent literature on sperm DNA fragmentation have consistently demonstrated that the degree of fragmentation strongly correlates with spermatozoa parameters such as concentration, motility, and morphology with correlation coefficients as high as 0.50 [11][12][13][14][15].…”

Section: Discussionmentioning

confidence: 78%

“…Although not investigated in the current analysis, the recent literature on sperm DNA fragmentation have consistently demonstrated that the degree of fragmentation strongly correlates with spermatozoa parameters such as concentration, motility, and morphology with correlation coefficients as high as 0.50 [11][12][13][14][15]. Hence it is possible that the postprocessing spermatozoa motility may be an index of cryoinjury and DNA fragmentation [8].…”

Section: Discussionmentioning

confidence: 88%

Optimization of IVF pregnancy outcomes with donor spermatozoa

Wang

Douglas

Prosser

et al. 2009

J Assist Reprod Genet

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Purpose To identify risk factors for suboptimal IVF outcomes using insemination with donor spermatozoa and to define a lower threshold that may signal a conversion to fertilization by ICSI rather than insemination. Method Retrospective, age-matched, case-control study of women undergoing non-donor oocyte IVF cycles using either freshly ejaculated (N=138) or cryopreserved donor spermatozoa (N=69). Associations between method of fertilization, semen sample parameters, and pregnancy rates were analyzed. Results In vitro fertilization of oocytes with donor spermatozoa by insemination results in equivalent fertilization and pregnancy rates compared to those of freshly ejaculated spermatozoa from men with normal semen analyses when the post-processing motility is greater than or equal to 88%. IVF by insemination with donor spermatozoa when the post-processing motility is less than 88% is associated with a 5-fold reduction in pregnancy rates when compared to those of donor spermatozoa above this motility threshold. When the post-processing donor spermatozoa motility is low, fertilization by ICSI is associated with significantly higher pregnancy rates compared to those of insemination. Conclusion While ICSI does not need to be categorically instituted when using donor spermatozoa in IVF, patients should be counseled that conversion from insemination to ICSI may be recommended based on low post-processing motility.

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Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity

Cited by 212 publications

References 25 publications

Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse

Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse

Effects of age on DNA double-strand breaks and apoptosis in human sperm

Optimization of IVF pregnancy outcomes with donor spermatozoa

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