Cryopreservation of human ovarian tissue using the silver closed vitrification system

Xiao, Zhun; Zhang, Yaoyao; Fan, Wei

doi:10.1007/s10815-017-1004-5

Cited by 9 publications

(6 citation statements)

References 44 publications

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“…Primary follicles as oocytes surrounded by a layer of cuboidal granulosa cells, and secondary with two or more layers of cuboidal granulosa cells [ 46 ]. Morphologically, follicles were subcategorized as healthy follicles or atretic follicles [ 47 , 48 ]. For area measurement Olympus BH-2 microscope with Visiopharm Integrator System software (Visiopharm, Hoersholm, Denmark, version 4.6.1779) was used.…”

Section: Methodsmentioning

confidence: 99%

Effects of needle puncturing on re-vascularization and follicle survival in xenotransplanted human ovarian tissue

Olesen¹,

Pors²,

Adrados³

et al. 2023

Reprod Biol Endocrinol

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Background Ovarian tissue transplantation can restore fertility in young cancer survivors, however the detrimental loss of follicles following transplantation of cryopreserved ovarian tissue is hampering the efficiency of the procedure. This study investigates whether needle puncturing prior to transplantation can enhance revascularization and improve follicle survival in xenotransplanted human ovarian cortex. Methods Cryopreserved human ovarian cortex pieces (N = 36) from 20 women aged 24–36 years were included. During the thawing process, each piece of tissue was cut in halves; one half serving as the untreated control and the other half was punctured approximately 150–200 times with a 29-gauge needle. The cortex pieces were transplanted subcutaneously to immunodeficient mice for 3, 6 and 10 days (N = 8 patients) and for 4 weeks (N = 12 patients). After 3, 6 and 10 days, revascularization of the ovarian xenografts were assessed using immunohistochemical detection of CD31 and gene expression of angiogenic factors (Vegfα, Angptl4, Ang1, and Ang2), and apoptotic factors (BCL2 and BAX) were performed by qPCR. Follicle density and morphology were evaluated in ovarian xenografts after 4 weeks. Results A significant increase in the CD31 positive area in human ovarian xenografts was evident from day 3 to 10, but no significant differences were observed between the needle and control group. The gene expression of Vegfα was consistently higher in the needle group compared to control at all three time points, but not statistically significant. The expression of Ang1 and Ang2 increased significantly from day 3 to day 10 in the control group (p < 0.001, p = 0.0023), however, in the needle group this increase was not observed from day 6 to 10 (Ang2 p = 0.027). The BAX/BCL2 ratio was similar in the needle and control groups. After 4-weeks xenografting, follicle density (follicles/mm3, mean ± SEM) was higher in the needle group (5.18 ± 2.24) compared to control (2.36 ± 0.67) (p = 0.208), and a significant lower percentage of necrotic follicles was found in the needle group (19%) compared to control (36%) (p = 0.045). Conclusions Needle puncturing of human ovarian cortex prior to transplantation had no effect on revascularization of ovarian grafts after 3, 6 and 10 days xenotransplantation. However, needle puncturing did affect angiogenic genes and improved follicle morphology.

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Section: Methodsmentioning

confidence: 99%

Effects of needle puncturing on re-vascularization and follicle survival in xenotransplanted human ovarian tissue

Olesen¹,

Pors²,

Adrados³

et al. 2023

Reprod Biol Endocrinol

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“…The absence almost of liquids and the difficulty to form ice crystals are attributed to the small volume during vitrification ( 149 ). In an attempt to avoid the larger volumes of CPAs, various tools have been described as for instance the solid surface ( 151 ), the medium droplets ( 152 ), the plastic straws ( 153 ), the open Cryo Type M, the closed CryoSheet device ( 154 ), and the silver closed vitrification system ( 155 ). Interestingly, the use of the stepped vitrification device with high DMSO concentrations resulted in reduced follicle membrane disintegration ( 156 ).…”

Section: Not Routinely Applied Assisted Reproductive Technologies For...mentioning

confidence: 99%

A comprehensive review and update on human fertility cryopreservation methods and tools

et al. 2023

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The broad conceptualization of fertility preservation and restoration has become already a major concern in the modern western world since a large number of individuals often face it in the everyday life. Driven by different health conditions and/or social reasons, a variety of patients currently rely on routinely and non-routinely applied assisted reproductive technologies, and mostly on the possibility to cryopreserve gametes and/or gonadal tissues for expanding their reproductive lifespan. This review embraces the data present in human-focused literature regarding the up-to-date methodologies and tools contemporarily applied in IVF laboratories' clinical setting of the oocyte, sperm, and embryo cryopreservation and explores the latest news and issues related to the optimization of methods used in ovarian and testicular tissue cryopreservation.

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“…Xiao et al vitrified ovarian tissue with three different systems: an open system with needle immersed vitrification (NIV), a closed plastic system, and a closed silver system. The silver closed system can better vitrify follicles with higher vitality, better morphology, and less risk of infection (24). Suzuki et al vitrified ovarian tissue combined with in vitro activation (IVA) for women with primary ovarian insufficiency (POI) and generated three pregnancies with two live births (20).…”

Section: Slow Freezing Versus Vitrificationmentioning

confidence: 99%

Ovarian tissue bank for fertility preservation and anti-menopause hormone replacement

et al. 2022

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Traditional fertility preservation methods such as embryo or oocyte cryopreservation cannot meet the needs of a cancer patient or for personal reasons. The cryopreservation of ovarian tissue can be an alternative and has become a hot spot to preserve fertility or hormone replacement. The freezing of ovarian tissue can be carried out at any time without ovarian hyperstimulation to retrieve follicles. It is an ideal strategy to preserve reproductive function in children, adolescent cancer patients, and patients who are in urgent need of cancer treatment. With the increasing demands of women with premature ovarian failure or in menopause, ovarian tissue transplantation is also an alternative for hormone replacement that can provide physiological doses of hormone levels, which can avoid a series of risks such as thrombosis, breast cancer, or other hormone-dependent tumors, caused by oral hormone replacement. Hence, ovarian tissue banking can be regarded as a mainstream strategy for fertility preservation and anti-menopause hormone replacement in further clinical investigation.

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Cryopreservation of human ovarian tissue using the silver closed vitrification system

Abstract: The hand-made silver container as a CVS is a promising carrier for the cryopreservation of the human ovarian cortex.

Cited by 9 publications

References 44 publications

Effects of needle puncturing on re-vascularization and follicle survival in xenotransplanted human ovarian tissue

Effects of needle puncturing on re-vascularization and follicle survival in xenotransplanted human ovarian tissue

A comprehensive review and update on human fertility cryopreservation methods and tools

Ovarian tissue bank for fertility preservation and anti-menopause hormone replacement

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