Cryopreservation of human lymphocytes and stem cells (CFU-c) in large units for cancer therapy—A report based on the data of more than 400 frozen units

Scheiwe, M.W.; Pusztai-Markos, Zs.; Essers, U.; Seelis, R.; Rau, G.; Körber, Ch.; Stürner, Klarissa Hanja; Jung, Han‐Sung; Liedtke, B

doi:10.1016/0011-2240(81)90107-3

Cited by 19 publications

(2 citation statements)

References 16 publications

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“…Scheiwe et al found that granulocytes were fragile and exquisitely sensitive to freezing/thawing and osmotic stress. Granulocytes could not survive cryopreservation like lymphocytes, and the damaged granulocytes might be the reason of aggregation [84]. Thus far, there is still no clinically available method to preserve granulocytes.…”

Section: Impacts Of Cryopreservation On Immune Cell Functionsmentioning

confidence: 99%

Progress in Cryopreservation of Stem Cells and Immune Cells for Cytotherapy

Shu¹,

Heimfeld²,

Huang³

et al. 2015

Progress in Stem Cell Transplantation

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Cellular therapy with stem and immune cells has demonstrated significant success both in clinical treatments and the industrial market. Cryopreservation is a necessary and essential component of cellular therapy. In this chapter, first of all, some basic theories of cryoinjury and techniques in cryopreservation are reviewed. Then it focuses on the progress of cryopreservation of stem cells and immune cells, including new protocols and techniques, alternative cryoprotective agents CP" , side effects after transplantation, and advances in reducing adverse reactions. Strategies to minimize adverse effects include medication before and after transplantation, optimizing the infusion procedure, reducing the CP" concentration or using alternative CP"s for cryopreservation, and removing CP" prior to infusion. Traditional and newly developed approaches including methods and devices for CP" removal are discussed. Future work is recommended including further optimization of cryopreservation protocols especially for lymphocytes standardization of the optimized protocols with temperature monitoring and quality control exploration of DMSO-free, serum-free, and even xeno-free media for cryopreservation development of simple, reliable, and cost-effective devices for cryopreservation and more fundamental cryobiological studies to avoid cellular injury

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Section: Impacts Of Cryopreservation On Immune Cell Functionsmentioning

confidence: 99%

Progress in Cryopreservation of Stem Cells and Immune Cells for Cytotherapy

Shu¹,

Heimfeld²,

Huang³

et al. 2015

Progress in Stem Cell Transplantation

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show abstract

“…For example, in the case of human white blood cells, the optimum cooling rate in a solution without cryoadditive (i.e. in physiological saline) has not yet been established, though these cells have widespread applications in medicine (Scheiwe et al, 1981a).…”

Section: Introductionmentioning

confidence: 99%

Thermally defined cryomicroscopy and some applications on human leucocytes

Scheiwe¹,

Körber²

1982

Journal of Microscopy

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SUMMARY A freezing‐stage has been developed for use on a standard light‐microscope, which can provide reproducible, precisely linear cooling and warming rates in the range from 0·1 to 10,000 K/min. Biological cells in aqueous solutions can be observed during the freeze‐thaw cycle; the volume loss due to osmotic efflux of water and the intracellular crystallization of water are detected by video‐monitoring. The temperature field generated in the observed samples is comparable to extended cylindrical probes and allows the transfer of cryomicroscopic data to technically used vial geometries. Lymphocytes and granulocytes were observed during freezing using the system described. They were separated and washed, and then frozen on the cold stage of the cryomicroscope at cooling rates ranging from 2 to 500 K/min. Shrinkage of the cells was observed up to 100 K/min and intracellular ice formation could be detected starting at 10 K/min. The results show that human leucocytes show excessive shrinkage up to 36% of their initial volume; the probability of intracellular ice formation exhibits a sharp increase from 10 to 100 K/min where nearly all cells contain ice.

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Biokältetechnik: Verfahren der Gefrierkonservierung in der Medizin

Scheiwe

Rau

1981

Chemie Ingenieur Technik

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Das Verfahren der Gefrierkonservierung beinhaltet die Überführung der flüssigen Substanz in eine feste, gefrorene Phase und die Umkehr dieses Vorganges. Das Ziel ist die möglichst vollständige Erhaltung der Ausgangseigenschaften des biologischen Materials, insbesondere der Lebensfähigkeit und der Ausbeute lebender Zellen, wie z. B. Blutkörperchen, die in der Medizin gezielt therapeutisch verwendet werden sollen. Diese Arbeit soll exemplarisch den Weg aufzeigen, der von der gestellten Aufgabe zur verfahrenstechnischen Lösung führt. Anhand der Physikochemie des Gefriervorganges der vorliegenden Kochsalzlösungen und involvierten, osmotisch reagierenden Zellen werden die Bedingungen für günstige Prozeßführung und die geschwindigkeitsbestimmenden Schritte dargelegt. Die hieraus folgenden technischen Problemstellungen sind Wärmetransport an Flüssig‐Stickstoff, instationäre Wärmeübertragung gefrierender Körper und Auslegungskriterien für technisches Gefrieren. Schließlich werden Gefrier‐Konservierungsprozesse und Ergebnisse der besonders aktuellen Anwendungsgebiete, nämlich rote Blutkörperchen für Katastrophenfälle und weiße Blutkörperchen für die Krebstherapie, aufgezeigt. Als konkretes Beispiel wird eine erst durch die Gefrierkonservierung ermöglichte Verbesserung der Tumortherapie aufgezeigt.

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Cryopreservation of human lymphocytes and stem cells (CFU-c) in large units for cancer therapy—A report based on the data of more than 400 frozen units

Cited by 19 publications

References 16 publications

Progress in Cryopreservation of Stem Cells and Immune Cells for Cytotherapy

Progress in Cryopreservation of Stem Cells and Immune Cells for Cytotherapy

Thermally defined cryomicroscopy and some applications on human leucocytes

Biokältetechnik: Verfahren der Gefrierkonservierung in der Medizin

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