Cryopreservation of human embryonic stem cells by a programmed freezer with an oscillating magnetic field

Lin, Pei-Yi; Yang, Yao-Chen; Hung, Shih‐Han; Lee, Sheng-Yang; Lee, Maw‐Sheng; Chu, I‐Ming; Hwang, Shiaw-Min

doi:10.1016/j.cryobiol.2013.02.061

Cited by 31 publications

(21 citation statements)

References 29 publications

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“…Usually, the ABI freezing protocol produces better results than conventional procedures. Thus, experiments performed in human stem cells, both dental pulp (Lee and others , ) and embryonic cells (Lin and others ), show that magnetic freezing is an efficacious method for stem cell cryopreservation. Lee and others () proved that the viability, attachment efficiency, and proliferation rate of magnetically frozen dental pulp stem cells were significantly greater than that of conventionally frozen cells.…”

Section: Experimental Data About the Effects Of Mfs On Freezingmentioning

confidence: 99%

Effects of Magnetic Fields on Freezing: Application to Biological Products

Otero

Rodríguez

Pérez‐Mateos

et al. 2016

Comp Rev Food Sci Food Safe

120

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Magnetic freezing is nowadays established as a commercial reality mainly oriented towards the food market. According to advertisements, magnetic freezing is able to generate tiny ice crystals throughout the frozen product, prevent cell destruction, and preserve the quality of fresh food intact after thawing. If all these advantages were true, magnetic freezing would represent a significant advance in freezing technology, not only for food preservation, but also for cryopreservation of biological specimens such as cells, tissues, and organs. Magnetic fields (MFs) are supposed to act directly on water by orientating, vibrating, and/or spinning molecules to prevent them from clustering and, thus, to promote supercooling. However, many doubts exist about the real effects of MFs on freezing and the science behind the potential mechanisms involved. To provide a basis for extending the understanding of magnetic freezing, this paper presents a critical review of the materials published in the literature up to now, including both patents and experimental results. After examining the information available, it was not possible to discern whether MFs have an appreciable effect on supercooling, freezing kinetics, ice crystals, quality, and/or viability of the frozen products. Experiments described in the literature frequently fail to identify and/or control all the factors that can play a role in magnetic freezing. Moreover, many of the comparisons between magnetic and conventional freezing are not correctly designed to draw valid conclusions, and wide ranges of MF intensities and frequencies are unexplored. Therefore, more rigorous experimentation and further evidence are needed to confirm or reject the efficacy of MFs in improving the quality of frozen products.

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Section: Experimental Data About the Effects Of Mfs On Freezingmentioning

confidence: 99%

Effects of Magnetic Fields on Freezing: Application to Biological Products

Otero

Rodríguez

Pérez‐Mateos

et al. 2016

Comp Rev Food Sci Food Safe

120

View full text Add to dashboard Cite

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“…The seven protocols evaluated in this study were either slow freezing ( n = 5) or vitrification methods ( n = 2), of which three are already clinically used for other cell types. Slow freezing is best studied and most widely used for any cell type or tissue . Cells are gradually (±−1 °C/min) exposed to low temperatures in the presence of CPAs down to −80 °C and thereafter transferred to liquid nitrogen.…”

Section: Introductionmentioning

confidence: 99%

Safe and effective cryopreservation methods for long‐term storage of human‐amniotic‐fluid‐derived stem cells

et al. 2015

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“…Lee et al reported that whole teeth which were cryopreserved by a CAS freezer can be used for autotransplantation and provide a viable source of human dental pulp stem cells [17]. Lin showed that human embryonic stem cells can maintain their pluripotency capacity and undifferentiated state after cryopreservation by a CAS freezer [19]. It was shown that a CAS freezer is useful for cryopreservation of human induced pluripotent stem cell-derived neural stem/progenitor cells [25].…”

Section: Introductionmentioning

confidence: 98%