Cryopreservation of human adipose tissues

Cui, Xianwei; Gao, Dayong; Fink, Betsy F.; Vásconez, Henry C.; Pu, Lee Li-Qun

doi:10.1016/j.cryobiol.2007.08.012

Cited by 55 publications

(44 citation statements)

References 30 publications

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“…79 In fact, cryopreservation of fat tissue is not a new research area and has been extensively investigated in large scale adipose tissue engineering, especially in applications of maxillofacial and craniofacial surgery. Although many studies found that freezing fat grafts by using optimal cryopreservation techniques enables their longterm preservation, [80][81][82][83][84][85][86] only few considered whether the frozen fat tissue could still be a reliable source of adult stem cells. 87,88 Further studies are required to determine whether cryopreserved fat tissue still maintains the viable and potent adult stem cells in quantities required for clinical needs.…”

Section: Clinical Banking Of Adipose Derived Adult Stem Cells (Ascs)mentioning

confidence: 99%

Clinical grade adult stem cell banking

2009

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Section: Clinical Banking Of Adipose Derived Adult Stem Cells (Ascs)mentioning

confidence: 99%

Clinical grade adult stem cell banking

2009

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“…The majority of studies performed to date have stressed the need for some type of cryopreservation to maintain adipocyte viability and graft integration [4,[20][21][22]. For example, Moscatello et al [19] concluded that adipocytes isolated from frozen adipose samples are not viable unless protected using cryopreservatives such as 10% DMSO (dimethylsulfoxide), 7.5% PVP (polyvinylpyrrolidone), or 10% glycerol.…”

Section: Discussionmentioning

confidence: 97%

Freezing Adipose Tissue Grafts May Damage Their Ability to Integrate into the Host

Grewal

Yacomotti

Melkonyan

et al. 2009

Connective Tissue Research

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In this study, the effect of freezing on the morphology, viability, and VEGF synthesis of human adipose tissue grafts is examined. Currently, storage of adipose grafts involves freezing in simple saline solutions. However, the effect of freezing on the morphology and function of adipose tissue remains unclear. As a result, this study attempts to determine whether freezing adipose grafts should be considered prior to soft-tissue augmentation. In this study, the freezing of adipose grafts in saline for only 24 hr resulted in morphological changes in vivo and affected their ability to synthesize VEGF. The use of a simple cryopreservation medium containing sucrose appeared to maintain VEGF synthetic levels by the grafts and improved both their morphology and retention in vivo. However, the benefits of this cryopreservation medium were directly linked to storage time as long-term storage did not result in any noticeable benefit to graft retention. Finally, as an alternative to freezing, adipose grafts were combined with human adipose-derived stem cells (ASCs) to determine if their presence could enhance in vivo graft structure. The presence of ASCs did appear to improve graft structure in vivo over the short term and was also capable of improving tissue morphology when combined with grafts frozen in PBS. In conclusion, the successful use of adipose grafts may require a closer examination of the graft's storage conditions and time. Specifically, it now appears that the practice of freezing in saline may not be advisable if graft viability, activity, and structure are to be maintained in vivo.

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“…In addition, this temperature change results in decreased survival rates of fibroblasts and kidney epithelial cells in a PD098059 (MAPK kinase inhibitor)-dependent manner (Chan et al, 1999). Research has been conducted to develop methods for adipose tissue preservation by using protective substances that prevent tissue injuries caused by changes in temperature (Cui et al, 2007). Activated Akt induces adipokine production (Barthel et al, 1997), and Akt and PI3K inactivation leads to apoptosis in L1 adipocytes (Reusch and Klemm, 2002).…”

Section: Discussionmentioning

confidence: 98%