Cryopreservation of hematopoietic progenitor cells from apheresis at high cell concentrations does not impair the hematopoietic recovery after transplantation

Abstract

The cryopreservation of HPC-A at higher than standard NC concentrations has no adverse impact on hematopoietic reconstitution after transplantation.

Autologous peripheral blood progenitor cells cryopreserved with 5 and 10 percent dimethyl sulfoxide alone give comparable hematopoietic reconstitution after transplantation

“…It is well known that engraftment is faster for the patients who receive higher total prefreeze or higher viable CD34+ cell doses 3,12,16,29,31‐33 . In our study, despite the fact that 5 percent MM patients were transplanted with fewer CD34+ cell doses, they did not show delayed hematologic engraftment.…”

Effect of high nucleated cell concentration on product viability and hematopoietic recovery in autologous transplantation

“…In the study by Cabezudo et al, a median cell concentration of 2.4 × 10 8 NC/mL (range of 1.5‐4.7 × 10 8 NC/mL) were collected and cryopreserved from 19 leukapheresis procedures on 10 patients. The higher cell concentration group of 238 patients in the study by Martin‐Henoa et al had a mean of 1.83 × 10 8 NC/mL (range of 0.16‐6.78 × 10 8 NC/mL). Both studies did not show any significant increases in time for neutrophil and platelet recovery.…”

Engraftment for CD34 selected stem cell products is not compromised by cryopreservation

“…Thus the incidence of acute or chronic GVHD was not compared between the fresh and cryopreserved cohorts; overall the incidence of chronic GVHD is very low in our haplocord transplants. Some studies have shown that cryopreserved PBSCs at concentrations higher than 2 × 10 8 cells/mL can cause cell viability loss, but CD34+‐selected products have very low cell concentration (<1 × 10 5 cells/mL).…”