Cryopreservation of hematopoietic progenitor cells with 5‐percent dimethyl sulfoxide at −80° C without rate‐controlled freezing

Abstract

This simplified cryopreservation technique will be useful for institutions without rate-controlled freezing facilities. Moreover, this method diminishes the amount of DMSO infused to patients, as well as its toxicity.

Long-term hematologic reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at 80 C in a mechanical freezer

“…Long-term observations in this study confirm previous data regarding the feasibility of cryopreserving stem cells in diminished DMSO concentrations; as low as 2.2% for stem cells cryopreserved in liquid nitrogen 15 or as low as 5% for stem cells frozen and stored at -80°C. 8,9,[16][17][18] This study also confirms that the elimination of HES as an extracellular cryoprotectant can be carried out within the cryopreservation time scale of less than 6 months without affecting long-term hematologic recovery. However, we recommend caution when considering longer periods of cryopreservation.…”

“…This protocol involved storage in the same mechanical freezer in solutions containing 5% or 10% DMSO as the sole cryoprotectant without HES. [7][8][9] Most studies seem to indicate that this simplified procedure is associated with a reduction in infusion toxicity and lower costs, with a similar hematopoietic reconstitution and clinical outcome to more standard protocols. However, there is little data regarding the long-term hematologic recovery and clinical course when such protocols are used.…”

A randomized phase III clinical trial of autologous blood stem cell transplantation comparing cryopreservation using dimethylsulfoxide vs dimethylsulfoxide with hydroxyethylstarch

“…24,25 The optimal concentration of either colligative cryoprotectant for the cryoprotection of HSC has not been determined in models of hematopoietic stem cell engraftment, although concentrations of DMSO as low as 5% have been used successfully for HCT. 26 In in vitro models, the optimal concentration of DMSO used alone appears to be about 10%. Ragab et al 27 studied the survival of HSC from human donors after freezing with various concentrations of DMSO.…”

Autologous Stem Cell Transplantation in the Treatment of Amyloidosis – Can Manipulation of the Autograft Reduce Treatment-Related Toxicity?

“…One other group used 3.5% DMSO, which is the lowest reported DMSO concentration in a clinical study but it was used in combination with 2.5% hydroxyethyl starch and followed by storage at -80°C (Halle et al, 2001). Galmés and colleagues, on the other hand, reported cryopreservation using only 5% DMSO and promptly hematopoietic engraftment as expected after short-time storage but slower hematopoietic recovery following long-time storage at -80°C (Galmes et al, 1996(Galmes et al, , 1999(Galmes et al, , 2007. Nevertheless, hematopoietic reconstitution was similar in both groups, when the patients were transplanted with more than 1.5 × 10 6 /kg CD34+ cells (Galmes et al, 2007).…”

“…There are only a few clinical studies where DMSO is used as the sole cryoprotectant at a 5 percent or lesser concentration (Akkok et al, 2009;Curcoy et al, 2002;Galmes et al 1996Galmes et al , 2007. One other group used 3.5% DMSO, which is the lowest reported DMSO concentration in a clinical study but it was used in combination with 2.5% hydroxyethyl starch and followed by storage at -80°C (Halle et al, 2001).…”