Cryopreservation of Germplasm of Chrysanthemums

Plant Cryopreservation: A Practical Guide

“…Generally shoot tips are 0.5-2 mm. Ahn 1995: Fukai andOe 1990;Fukai et al 1994;Fukai 1995 Controlled rate cooling 0.25°C/min; ED; Dropletfreezing 7.5% DMSO; Droplet vitrification PVS2…”

“…Callus production or detailed regenerant analyses were mentioned by Fukai (1990Fukai ( , 1995 and Fukai and Oe (1990) for chrysanthemum, and by Demeulemeester et al (1992) for chicory. Wilkinson et al (1998Wilkinson et al ( , 2003 studied topological ultrastructure and genetic stability of Cosmos.…”

Anthemideae: advances in tissue culture, genetics and transgenic biotechnology

“…Storage of ornamental chrysanthemum (and to a limited extent other members of the Anthemideae) genetic resources has been achieved through cryopreservation, low temperature preservation, and room temperature preservation (Fukai, 1995) in which the successful cryopreservation of shoot tips involves the ability to regenerate thawed shoots as well as maintaining their genetic composition. CCP = co-culture period in the light (L) or dark (D); OD = optical density (λ = wavelength of spectrophotometer).…”

Studies Regarding a Varietal Assortment of Potted Chrysanthemum

“…Due to the diversity of shapes and sizes of the inflorescence, the multitude of colors, its suitability for modern homes, make this flower species to be highly appreciated by the general public. Kitamura (Compositae, Anthemideae), originates from the Greek krus anthemon, meaning gold flower (Fukai, 1995), originally from China and Japan, known since 1500 BC. as a vegetable plant for the consumption of leaves, without a doubt chrysanthemum is considered today `queen of autumn flowers` (Toma, 2009).…”