Cryopreservation of embryogenic callus of<i>Arundo donax</i>L.

Özüdoğru, Elif Aylin; Roncasaglia, R.; Silva, D.P. Correa da; Moreira, Fernanda da Conceição; Lambardi, Maurizio

doi:10.17660/actahortic.2016.1113.4

Cited by 3 publications

(1 citation statement)

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“…A method for callus preservation is cryo-conservation by one-step vitrification in liquid nitrogen, in vials containing a protective solution with MS salts, sugar, DMSO, glycerol and ethylene glycol. The loss of growth caused by the treatment is below 10% [91]. In most papers, totipotent calli of A. donax are often described as embryogenic calli, corresponding to light yellow-white calli, with granular friable consistency.…”

Section: Somaclonal Variation and Mutagenesismentioning

confidence: 99%

Genetic Improvement of Arundo donax L.: Opportunities and Challenges

Danelli

Laura²,

Savona³

et al. 2020

Plants

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Arundo donax L., the giant reed—being a long-duration, low-cost, non-food energy crop able to grow in marginal lands—has emerged as a potential alternative to produce biomass for both energy production, with low carbon emissions, and industrial bioproducts. In recent years, pioneering efforts have been made to genetically improve this very promising energy crop. This review analyses the recent advances and challenges encountered in using clonal selection, mutagenesis/somaclonal variation and transgenesis/genome editing. Attempts to improve crop yield, in vitro propagation efficiency, salt and heavy metal tolerance by clonal selection were carried out, although limited by the species’ low genetic diversity and availability of mutants. Mutagenesis and somaclonal variation have also been attempted on this species; however, since Arundo donax is polyploid, it is very difficult to induce and select promising mutations. In more recent years, genomics and transcriptomics data are becoming available in Arundo, closing the gap to make possible the genetic manipulation of this energy crop in the near future. The challenge will regard the functional characterization of the genes/sequences generated by genomic sequencing and transcriptomic analysis in a complex polyploid genome.

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Section: Somaclonal Variation and Mutagenesismentioning

confidence: 99%