Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender

Hermansson, Ulrika; Johannisson, Anders; Axnér, Eva

doi:10.1002/vms3.445

Cited by 9 publications

(5 citation statements)

References 40 publications

(61 reference statements)

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“…En otro estudio se probaron distintas combinaciones de crioprotectores extracelulares e intracelulares y los resultaron mostraron que el uso de YH en combinación con glicerol eran óptimos para una criopreservación exitosa [9]. Se estudió la criopreservación de semen de perro en un diluyente Tris con dos preparaciones diferentes de soja al 1 % en comparación con un diluyente de YH, y se concluyó que la YH fue superior a cualquiera de los dos diluyentes a base de lecitina de soja utilizados en dicho estudio [16]. TABLA II Características del semen de perro post descongelación, sistema CASA A pesar de los resultados positivos con el uso de YH se buscan alternativas para minimizar los efectos adversos que ésta pueda causar.…”

Section: Resultados Y Discusiónunclassified

Evaluación de dos diluyentes en la congelación de espermatozoides epididimarios de perros (Alcohol poli vinílico y Yema de huevo)

et al. 2022

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La yema de huevo (YH) es un componente común de los diluyentes de semen por su eficacia para proteger la membrana de los espermatozoides del choque de frío durante la criopreservación y además de ser rica en proteínas, vitaminas, antioxidantes y fosfolípidos. No obstante, la YH es un componente no definido y al mismo tiempo peligroso por el riego de transmisión de agentes patógenos, por esta razón, es necesario evaluar el uso de otros componentes que disminuyan este problema; una alternativa poco estudiada es alcohol poli vinílico (APV). Por lo que en esta investigación se evaluó el efecto del APV y la YH suplementados al diluyente TCG (tris, ácido cítrico, glucosa) sobre la criosupervivencia de espermatozoides epididimarios (EE) de perro. Para ello, se recuperaron EE mediante la técnica de flujo retrógrado en 15 perros adultos orquiectomizados para conformar cinco agrupaciones (4-6 muestras epididimarias / agrupación). Cada muestra agrupada se dividió en dos alícuotas, que posteriormente fueron diluidas y congeladas usando dos aditivos suplementados al diluyente TCG: APV al 1 % (peso/volumen –p/v– [TCG–APV] y yema de huevo al 20 % (volumen/volumen –v/v– [TCG–YH]. Posterior a la descongelación, se analizaron las anormalidades morfológicas, la vitalidad (eosina / nigrosina), la integridad de membrana plasmática (ioduro de propido), y las variables cinemáticas (sistema CASA, SCA-2018®). Los resultados demostraron que la vitalidad e integridad de la membrana plasmática fueron superiores (P<0,05) en muestras congeladas con TCG–YH, en comparación con aquellas muestras congeladas con TCG–APV. Asimismo, las muestras congeladas con TCG–YH obtuvieron valores post-descongelación más altos (P<0,05) de variable cinemáticas como la motilidad total y progresiva, velocidades promedio y rectilínea, índice de rectitud y frecuencia de batida de flagelo, en comparación con las muestras congeladas con TCG–APV. En conclusión, la adición YH al medio TCG fue efectiva en la congelación de EE de perro, ya que mejoró la vitalidad, integridad de membrana plasmática y la cinética espermática; sin embargo, la adición de 1 % de APV no mejoró la respuesta a la criopreservación.

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Section: Resultados Y Discusiónunclassified

Evaluación de dos diluyentes en la congelación de espermatozoides epididimarios de perros (Alcohol poli vinílico y Yema de huevo)

et al. 2022

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“…Skim milk in combination with raffinose resulted in a sufficient litter size of 5.3 (Asano et al ., unpublished data) and 6.0 [ 36 ] after artificial insemination ( Table 1 ). Milk [ 24 ], egg yolk plasma [ 31 , 37 , 38 , 39 ], lecithin [ 11 , 32 , 33 , 34 , 40 ], lipoprotein [ 41 ], and liposomes [ 38 ] appear to have comparable or better effects on sperm motility after thawing, as alternatives to egg yolk. However, the results varied between the studies.…”

Section: Extender For Canine Spermatozoamentioning

confidence: 99%

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

Suzuki

Watanabe

Abe

2022

Journal of Reproduction and Development

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Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. Their widespread use has contributed to improvements in human health and welfare. However, in dogs, only artificial insemination using frozen semen is readily available as an ART to improve breeding and control genetic diversity. A recent priority in sperm cryopreservation is the development of alternatives to egg yolk, which is widely used as a component of the sperm extender. Egg yolk can vary in composition among batches and is prone to contamination by animal pathogens. The latter can be a problem for international exchange of cryopreserved semen. Low-density lipoprotein and skim milk are promising candidates for use as extenders, to ensure fertility after artificial insemination. Although not tested for its effects on fertility following artificial insemination, polyvinyl alcohol may also be a useful alternative to egg yolk as an extender. The development of cryopreservation techniques for canine embryos lags behind that for other mammals, including humans. However, given the success of non-surgical embryo transfer in 2011, studies have sought to refine this approach for practical use. Research on sperm cryopreservation has yielded satisfactory results. However, investigation of other approaches, such as cryopreservation of oocytes and gonadal tissues, remains insufficient. Techniques for the efficient induction of estrus may aid in the development of successful canine ARTs.

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“…Semen was collected from all dogs twice a week using the manual stimulation method (Puja et al, 2019). Only samples with a minimum of 70 motility, the normal morphology of spermatozoa of ≥ 70%, and the total sperm concentration ≥ 600 × 106 were considered for this study (Hermansson et al, 2021).…”

Section: Sample Collectionmentioning

confidence: 99%

“…The compositions used are shown in Table 1. A Tris egg yolk extender was prepared and modified according to the procedure described by Hermansson et al (2021).…”

Section: Extendersmentioning

confidence: 99%

Comparative Analysis of One-step and Two-step Dilution on Quality of Frozen Semen in Kintamani Dogs

Sulabda¹,

Dharmayudha²,

Puja³

2022

WVJ

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Preservation of sperm by freezing allows breeding dogs that are separated over long distances. To increase the fertility of frozen and then thawed spermatozoa, they must be able to survive the process. The current study aimed to evaluate the sperm motility and DNA integrity of Kintamani dogs extended in extenders with one-step and two-step dilution techniques. Ejaculates collected from four dogs were used in the current study. The semen was divided into two equal parts and diluted with extenders using two different dilution techniques, namely One-step dilution in Tris egg yolk containing 7% glycerol, and a two-step dilution technique diluted in an initial 2:1 with an extender, containing 20% egg yolk without glycerol. The same volume of the second extender was added, including 14% glycerol. The sample was loaded into 0.25 ml straws, cooled to 4°C for 4 hours, equilibrated, and then plunged into the liquid nitrogen. The sperm motility was evaluated using Computer-Assisted Sperm Analysis (CASA), and DNA integrity was assessed using Acridine Orange (AO) stained. Results showed that the sperm motility of Kintamani dogs in extenders using two-step dilution was significantly higher compared to the one-step dilution technique. In addition, the obtained results indicated that two types of dilution steps in Kintamani dog semen were not detrimental to the sperm DNA integrity during the freezing process. In conclusion, extenders with two types of dilution techniques could maintain sperm motility above 30%, and no difference between one and two steps dilution was detected.

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Cryopreservation of dog semen in a Tris extender with two different 1% soybean preparations compared with a Tris egg yolk extender

Cited by 9 publications

References 40 publications

Evaluación de dos diluyentes en la congelación de espermatozoides epididimarios de perros (Alcohol poli vinílico y Yema de huevo)

Evaluación de dos diluyentes en la congelación de espermatozoides epididimarios de perros (Alcohol poli vinílico y Yema de huevo)

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

Comparative Analysis of One-step and Two-step Dilution on Quality of Frozen Semen in Kintamani Dogs

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