Cryopreservation of Desiccation-Tolerant Seeds

Pritchard, Hugh W.

doi:10.1007/978-1-59745-362-2_13

Cited by 32 publications

(24 citation statements)

References 29 publications

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“…Storage at temperature below -160°C is expected to prolong seed life for hundreds or even thousands of years due to very high reduction of metabolic activities (Walters et al 2004). The most critical factor affecting cryopreservation of seeds is water content (WC), (Pritchard 2007). When dried to an optimal range of WC, the desiccation of seeds prior to cryopreservation in LN confers a significant increase in survival (Engelmann 2000).…”

Section: Introductionmentioning

confidence: 99%

Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.), a disappearing tree species in Europe

et al. 2014

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Black poplar (Populus nigra L.) is a widely distributed species that plays a crucial role in riparian forest ecosystems. Due to a reduction in its natural habitats and hybridization with introduced poplar species clones, its genetic pool is decreasing and efforts are required to preserve this species. Seeds of black poplar are short-lived and quickly lose viability during conventional storage in gene banks. Therefore, in order to preserve ex situ the genetic diversity of this species, the feasibility of seed cryopreservation in liquid nitrogen (at -196°C, LN) for periods of 24 h and 2 years was investigated. Seeds were harvested from three individual trees (two provenances) and desiccated to different levels of water content (WC) in the range of 0.02-0.35 g g -1 (g H 2 O/g dry mass, g g -1 ) prior to immersion in LN. Seed germination was assessed after each treatment. P. nigra seeds tolerated desiccation to WC 0.07 g g -1 but after severe desiccation to WC\0.05 g g -1 exhibited a significant reduction in germination. Results indicated that all black poplar seeds, regardless of origin, year of harvesting and seed quality, could be cryopreserved for 24 h when their WC was in the range of 0.11-0.17 g g -1 . Physiology of P. nigra seeds showed in this paper is consistent with attributes of intermediate seed storage behavior. This study provides a foundation for using cryopreservation for the ex situ conservation of P. nigra seeds.

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Section: Introductionmentioning

confidence: 99%

Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.), a disappearing tree species in Europe

et al. 2014

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“…However, alternative physical temperature monitoring methods for key locations will generally be adequate and the technology has been developed to produce devices that could record and report the temperature experienced by individual vials or ampoules of cells in storage over time [79]. In some circumstances, accelerated thermal stability studies are used to predict the survival of stored biological materials at ultra-low temperature [28,80,81].…”

Section: Other Issues For Long-term Storagementioning

confidence: 99%

“…Many BRCs provide vital services for ex situ conservation of biodiversity through the preservation, storage, and documentation of endangered algae [26], plant tissues [27], seeds [28], and animal gametes and embryos [29,30]. Such resource centers could potentially regenerate species that are lost to their natural habitat.…”

Section: Long-term Roles For Biological Resource Centresmentioning

confidence: 99%

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Biobanking

Day

Stacey

2008

Mol Biotechnol

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Biobanks, more formally known as biological resource centers (BRCs), form an ''unsung'' yet critical component of the infrastructures for scientific research, industry and conservation, without which much of the current scientific activity involving microbial cultures and celllines would be effectively impossible. BRCs are de facto depositories of ''biological standards'' holding taxonomic and other reference strains on which much of the associated published science and industrial standards are built and upon which some significant international commercial and ethical issues rely. The establishment and maintenance of BRCs is a knowledge-and skill-rich activity that in particular requires careful attention to the implementation of reliable preservation technologies and appropriate quality assurance to ensure that recovered cultures and other biological materials perform in the same way as the originally isolated culture or material. There are many types of BRC, which vary both in the kinds of material they hold and in their functional role. All BRCs are expected to provide materials and information of an appropriate quality for their intended use and work to standards relevant to those applications. There are important industrial, biomedical, and conservation issues that can only be addressed through effective and efficient operation of BRCs in the long term. This requires a high degree of expertise in the maintenance and management of collections of biological materials at ultra-low temperatures, or as freeze-dried material, to secure their long-term integrity and relevance for future research, development, and conservation.

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“…Cryopreservation has been used for seed and shoot-tip conservation, providing long-term storage (Pritchard 2007;Engelmann 2011a, b;Reed et al 2011). Cryopreservation is the best long-term approach to preserving endangered species (Engelmann 2000).…”

Section: Introductionmentioning

confidence: 99%

Seed cryopreservation and micropropagation of the critically endangered species swamp pink (Helonias bullata L.)

Perullo

Determann²,

Cruse-Sanders³

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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Helonias bullata, commonly known as swamp pink, is a perennial rhizomatous herb native to the eastern coast of the United States that is found mainly in forested wetlands, swamps, and mountain bogs. Habitat destruction has caused H. bullata to become rare, and it is federally listed as threatened. Protocols were developed for in vitro germination, shoot micropropagation, shoot establishment in soil, and seed cryopreservation that will assist in the safeguarding and conservation of dwindling natural populations. Seeds were germinated in vitro on plant growth regulator (PGR)-free 1/3-strength Murashige and Skoog (MS) medium after seed sterilization in H 2 O 2 and used for establishment of shoot cultures. Shoot multiplication and elongation occurred on 1/3-strength MS medium containing 0.25-1 mg/l kinetin or 1-2 mg/l trans-zeatin followed by growth on PGR-free medium. All shoots (100%) rooted when planted directly into potting media. Rapid-immersion seed cryopreservation resulted in statistically significant increases in germination in vitro or in potting mix compared to control treatments. H. bullata seeds share characteristics of both orthodox and recalcitrant seeds including rapid loss of viability over time, survival at 5°C, and survival after cryopreservation. Therefore, they are most accurately classified as intermediate-type seed.

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Cryopreservation of Desiccation-Tolerant Seeds

Cited by 32 publications

References 29 publications

Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.), a disappearing tree species in Europe

Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.), a disappearing tree species in Europe

Biobanking

Seed cryopreservation and micropropagation of the critically endangered species swamp pink (Helonias bullata L.)

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