Cryopreservation of Date Palm (Phoenix Dactylifera L.) Cultured in Vitro

Bekheet, S. A.; Taha, Hussein; Solliman, Mohei El-Din; Hassan, N.A.

doi:10.17660/actahortic.2007.736.26

Cited by 17 publications

(16 citation statements)

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“…Micropropagation has been successfully achieved either through direct or indirect morphogenesis for different date palm cultivars (Mater 1986;Taha et al 2001;Tisserat 1984;Zaid and Tisserat 1983). The procedures developed can also be utilized for conservation by inducing medium-and longterm preservation (Bekheet et al , 2007.…”

Section: Constraints Of Traditional Preservationmentioning

confidence: 99%

“…Three types of meristematic and regenerative in vitro tissues can be obtained from date palm: (1) individual meristems isolated from shoot-tip cultures; (2) highly proliferating meristem cultures containing nodular-like meristem clusters; (3) somatic embryos. Cryopreservation methods have been developed for nodular tissue by Bekheet et al (2007). Undifferentiated tissue cultures (nodular cultures) were successfully cryopreserved by freezing methods; subsequently the plants were regenerated.…”

Section: Development Of Date Palm Cryopreservationmentioning

confidence: 99%

“…During culture, prior to the cryoprotective treatment, obvious starch synthesis occurred in some cells of the samples. RAPD analysis was used to examine the genetic stability of cryo-stored date palm tissue cultures (Bekheet et al 2007). Both treated and non-treated date palm tissue cultures in addition to field grown plants were identical with the primers used (Fig.…”

Section: Genetic Stability Of Conserved Collectionsmentioning

confidence: 99%

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In Vitro Conservation of Date Palm Germplasm

Bekheet

2011

Date Palm Biotechnology

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Date palm (Phoenix dactylifera L.) germplasm is difficult to conserve and store in the form of offshoots or in field collections. Tissue culture technologies have had a major impact on the ex situ conservation of plant genetic resources. In vitro culture techniques supplement date palm conservation efforts and have been applied to germplasm collection, preservation and rapid clonal multiplication. In vitro storage methods have been developed for preservation of date palm germplasm and can be used efficiently for international exchange of germplasm because of their obvious advantages over in vivo material. Preservation of plant cells, meristems and somatic embryos has become an important tool for long-term storage of germplasm utilizing minimum space and low maintenance. Short-and mid-term storage is achieved by controlling environmental growth conditions and nutrient media composition. Long-term storage has been reported for in vitro cryopreservation of date palm cultures. Encapsulation of plant material in alginate beads has been suggested recently as a possible means of date palm germplasm exchange. Knowledge about germplasm diversity and genetic relationships are highly valuable tools in plant conservation strategies. In this regard, a number of molecular biology methods are currently available for analysis of genetic diversity in date palm genotypes. This chapter discusses the general issues and different aspects of plant biotechnology used for management and conservation of date palm cultivars.

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Section: Constraints Of Traditional Preservationmentioning

confidence: 99%

Section: Development Of Date Palm Cryopreservationmentioning

confidence: 99%

Section: Genetic Stability Of Conserved Collectionsmentioning

confidence: 99%

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In Vitro Conservation of Date Palm Germplasm

Bekheet

2011

Date Palm Biotechnology

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“…A genuine micropropagation method is the primary requirement of cryopreservation of germplasm and in vitro conservation. Over decades researchers have standardized date palm micropropagation and started the cryopreservation of date palm tissue (Bekheet et al, 2007). Embryogenic calli of date palm were treated with cryogen and stored for several months under ultra-low temperature (-196 ºC).…”

Section: Cryopreservationmentioning

confidence: 99%

Date palm micropropagation: Advances and applications

Al-Khayri

Naik

2017

Ciênc. agrotec.

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Date palm (Phoenix dactylifera L.) is a fruit tree resilient to adverse climatic conditions predominating in hot arid regions of the Middle East and North Africa. The date fruit contains numerous chemical components that possess high nutritional and medicinal values. Traditional propagation by offshoots is inefficient to satisfy current demands for date palm trees. Alternatively, micropropagation provides an efficient means for large-scale propagation of date palm cultivars. Both somatic embryogenesis and organogenesis, either directly or indirectly though the callus phase, have been demonstrated in date palm in vitro regeneration. Culture initiation commonly utilizes shoot-tip explants isolated from young offshoots. Recently, the immature inflorescences of adult trees were utilized as an alternative nondestructive source of explants. In addition to the nature of the explant used, successful plant regeneration depends on the cultivar, composition of the culture medium and physical status. Challenges of date palm micropropagation include long in vitro cycle, latent contamination, browning, somaclonal variation as well as ex vitro acclimatization and transplanting. A remarkable amount of research investigating these factors has led to optimized protocols for the micropropagation of numerous commercially important cultivars. This has encouraged the development of several international commercial tissue culture laboratories. Molecular characterization provides an assurance of genetic conformity of regenerated plantlets, a key feature for commercial production. This article describes date palm micropropagation protocols and also discusses recent achievements with respect to somaclonal variation, molecular markers, cryopreservation and future prospects.Index terms: Cryopreservation; somatic embryogenesis; somaclonal variation; organogenesis; molecular marker. RESUMOA tamareira (Phoenix dactylifera L.) é uma arvore frutífera adaptada à condições climáticas adversas predominantemente em regiões áridas do Oriente Médio e Norte Africano. As tâmaras possuem vários componentes químicos com alto valor medicinal e nutricional. A propagação tradicional por estacas não é suficiente para satisfazer a demanda por mudas e assim, a micropropagação apresenta-se como uma alternativa eficiente para a produção de mudas em larga escala. Embriogênese somática e organogênese, tanto direta quanto indireta via calos, tem sido usada para obter a regeneração in vitro de tamareira. O inicio do cultivo in vitro normalmente utiliza meristemas excisados de brotações jovens. Recentemente, inflorescências imaturas de árvores adultas são usadas como fonte alternativa de explantes não destrutiva. Além da origem do explante, o sucesso da regenerção depende do cultivar, da composição do meio de cultura e de condições físicas. Desafios na micropropagação de tamareira incluem um longo ciclo in vitro, contaminação, escurecimento do tecido, variação somaclonal além do enraizamento e aclimatização ex vitro. Diversos estudos investigando esses fatore...

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“…Such germplasm material is developmentally synchronized, miniaturized, and potentially genetically homogenous (Al-Bahrany and Al-Khayri 2012 ). Although such germplasm can be both rapidly and massively propagated and exchanged, however, its phytosanitary status may not be guaranteed, except in the case of shoot tip or meristem cultures (Bekheet et al 2007 ), and its potential for genetic uniformity of elite cultivars at the expense of less-preferred ones should not be overlooked (Jaradat 2011 ).…”

Section: In Vitro Conservation Of Date Palm Genetic Resourcesmentioning

confidence: 99%

Biodiversity, Genetic Diversity, and Genetic Resources of Date Palm

Jaradat

2015

Date Palm Genetic Resources and Utilization

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Phoenix dactylifera L. is composed of genetically discrete clones representing thousands of cultivars without the benefi ts of a dynamic mutationrecombination system; its genetic resources are the most important component of biodiversity in its natural habitats; these include modern cultivars, landraces, obsolete cultivars, breeding lines, and related wild species. Cultivated Phoenix is closely related to a variable aggregate of wild and feral palms distributed over a wide desert belt across the Middle East and North Africa. Genetic diversity and genetic structure of the species gene pool complex have been shaped and greatly altered by human and natural selection, clonal propagation, and spatiotemporal exchange of germplasm. The mixed sexual-clonal propagation system acted on the sexual traits, impacted the genetic structure of populations, and may have resulted in the accumulation of domesticated traits in the date palm. Traditional oases continue to play a vital role in the maintenance and enrichment of date palm biodiversity, genetic diversity, and genetic resources through multiple processes and dynamic conservation practices. However, with the advent of modern plantations emphasizing elite cultivars, a better understanding of the intraspecifi c genetic variation of date palm and its distribution in oasis agroecosystems is essential for the conservation and sustainable utilization of its biodiversity and genetic resources. In-depth assessment of the genetic vulnerability of date palm to biotic and abiotic stress requires knowledge of the extent and distribution of its genetic diversity, both of which depend on the species evolution and its unique breeding system, past genetic bottlenecks, and ecological, environmental, and anthropogenic factors.

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Cryopreservation of Date Palm (Phoenix Dactylifera L.) Cultured in Vitro

Cited by 17 publications

References 0 publications

In Vitro Conservation of Date Palm Germplasm

In Vitro Conservation of Date Palm Germplasm

Date palm micropropagation: Advances and applications

Biodiversity, Genetic Diversity, and Genetic Resources of Date Palm

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