Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

Surenciski, Mauro Rodrigo; Flachsland, Eduardo Alberto; Terada, Graciela; Mroginski, L. A.; Rey, H. Y.

doi:10.32604/biocell.2012.36.031

Cited by 22 publications

(7 citation statements)

References 5 publications

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“…Immature seeds of orchids would not survive under cryopreservation treatments without the use of cryoprotectant (Surenciski et al, 2012), which is consistent with our results. Kolahdoozan et al (2009) reported a higher germination percentage for the seeds of Medicago rigidula, cryopreserved in liquid nitrogen.…”

Section: Discussionsupporting

confidence: 92%

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

2014

Int. J. Biosci.

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Satureja rechingeri, belonging to the Lamiaceae family, is one of the native range species of Iran. Considering the loss of Satureja rechingeri germplasm, due to the limited distribution, biotic and abiotic stresses, overgrazing of livestock, and generally the risks threatening the habitat of this species, fundamental actions need to be performed towards the preservation of Satureja rechingeri genetic diversity. Cryopreservation has the ability to preserve the seeds for a long time with much lower costs than other methods and without loss of seed viability. In the current study, treatments of vitrification, glycerol (30%), and the reduction of seed moisture before entering the liquid nitrogen were used. After one week, the treated seeds were removed from liquid nitrogen and were melted in sterile distilled water bath with a temperature of 42 °C. Then, the seeds were washed by sucrose solution (1.2 M) using shaker in three stages and were cultured with control seeds on sterile wet filter papers and transferred to a growth chamber. According to the obtained results of seed cryopreservation of Satureja rechingeri, no significant difference was found for all germination and seed growth characteristics except germination percentage and rate. Our results clearly indicate the effectiveness of this method for the seed preservation of Satureja rechingeri as a unique and endangered species for a very long time.

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Section: Discussionsupporting

confidence: 92%

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

2014

Int. J. Biosci.

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“…The encapsulationdehydration techniques are generally applied to a few orchid preservations viz. Cyrtopodium hatschbachii and Oncidium bifolium (Flachsland et al, 2006;Surenciski et al, 2012).…”

Section: Encapsulation-dehydrationmentioning

confidence: 99%

Biotechnological interventions in Orchids: Recent updates, Translational success, and Commercial outcomes

Tiwari

Sharma

Bose

et al. 2022

Preprint

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Orchids constitute the largest and most diverse group of flowering plants and are classified in Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plants, the cultivation of orchids on a commercial level, is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has been successful in generating employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably improved the knowledge in areas of orchid biology, classification, phytochemistry, and cultivation strategies, among other areas. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, witnessed as cultivation of different varieties and globally marketed as a cut flower and artificially propagated plants. The orchids constitute the first floriculture crops, which revolutionized the orchid industry, however, it also withholds several challenges in natural propagation, several species facing extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming towards conservation and legal commercial goals. This thematic review is one of a kind in providing comprehensive insights on the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming time. Together with traditional breeding approaches and plant tissue culture, advances in biotechnological interventions continues to make substantial progress in the development of exotic varieties, with multi-faceted attributes. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach for the conservation and commercialization of orchids.

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“…Current work in cryopreservation has permitted the storage of virtually all explant types for many plant species (Ashmore et al 2011;Engelmann 2004;2011;González-Arnao and Engelmann 2006;González-Arnao et al 2008;2017;Panis and Lambardi 2006;Uragami 1993). In orchids, cryopreservation has been an efficient means of conserving seeds and pollen (e.g., Dolce et al 2016;Flachsland et al 2006;Hay et al 2010;Koopowitz 1986;Koopowitz and Thornhill, 1994;Mweetwa et al 2007;Popov et al 2004;Pritchard 1984;Vendrame et al 2007b;Surenciski et al 2012). By contrast, attempts to cryopreserve somatic explants (i.e., clonal material) are scarce and have resulted in variable regrowth González-Arnao et al 2009;Hernández-Ramírez et al 2014;Kondo et al 2001;Lurswijidjarus and Thammasiri 2004;Na and Kondo 1996;Thinh and Takagi 2000;Tsukazaki et al 2000).…”

Section: In Vitro Conservation Approaches In Orchidsmentioning

confidence: 99%

“…Their flowers (3-3.5 cm in diameter) are reddish, pink or pinkish with a yellowish labelum, in racemose inflorescences (with 8-15 flowers) which bloom in late winter. The callus labelum is used to identify the species of this genus (Surenciski et al 2012). Due to the restricted distribution of C. hatschbachii natural populations, its conservation acquires ecological significance.…”

Section: Genus: Cyrtopodiummentioning

confidence: 99%

“…In spite of the recognized limited morphogenetic competence of root-tip of higher plants, the utility of root explants for orchid micropropagation purposes is being increasingly realized due to their year round availability, low oxidation rate, and the ease with which they can be explanted (Chugh et al 2009). 3-Seed cryopreservation: The aim of this study was to adjust a cryopreservation protocol for immature seeds of C. hatschbachii using the encapsulation-dehydration technique (Surenciski et al 2012). Sterile immature seeds were encapsulated in 3% sodium alginate polymerized with calcium chloride (CaCl 2 ) at 0.1 M. Encapsulated seeds were then pre-treated in liquid half-strength MS enriched with a progressively increasing sucrose concentration, using the following sequence: 0.08 M (24 h); 0.15 M (24 h); 0.25 M (48 h); 0.5 M (24 h) and 0.75 M (24 h).…”

Section: Genus: Cyrtopodiummentioning

confidence: 99%

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In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

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Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

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Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration

Cited by 22 publications

References 5 publications

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

Biotechnological interventions in Orchids: Recent updates, Translational success, and Commercial outcomes

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

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