Cryopreservation of bull spermatozoa alters the perinuclear theca

Martínez, Carmen Omega; Juárez-Mosqueda, Marı́a de Lourdes; Hernández-Valdés, Jorge; Valencia, Javier

doi:10.1016/j.theriogenology.2005.10.028

Cited by 14 publications

(8 citation statements)

References 35 publications

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“…Additionally, Marquez & Ogasawara (1977) and Hammerstedt & Graham (1992) have demonstrated that glycerol has harmful effects on the acrosome and middle piece. Previous reports from this laboratory (Gutiérrez-Pérez 2006; Martínez et al ., 2006; Arancibia-Salinas et al ., 2007; Barrientos–Morales et al ., 2009) have revealed a relationship between PTS and acrosome integrity. However, although there is evidence of alteration of the cytoskeleton there were no reports of the possible direct effects of glycerol on the PTS of the boar spermatozoid.…”

Section: Discussionmentioning

confidence: 99%

“…In relation to the loss of fertility associated with cryopreserved boar spermatozoa, damage to the acrosome has been attributed to the combined effect of the glycerol concentration, and the cooling rate (Wilmut & Polge, 1974; Fiser et al ., 1993; Meyers, 2005; Si et al ., 2006). However, recently it was demonstrated that alterations in the sperm head cytoskeleton (perinuclear theca, PT) of spermatozoa from bovine and boar are related to the loss of the acrosome (Martínez et al ., 2006; Barrientos-Morales et al ., 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Juárez & Mújica (1999) reported the formation of a perinuclear theca substructure (PTS) in the apical region of the postacrosomal sheath of the PT in spermatozoa from several species, including boar (Juárez & Mújica, 1999; Juárez-Mosqueda, 2000); significantly, actin forms part of the PTS. Moreover, it has been reported that the PT of boar and bovine spermatozoa undergo alterations after the cryopreservation process (Martínez et al ., 2006; Orozco–Benítez et al ., 2008). Even though in other cell types it has been reported that cytoskeletal proteins may undergo changes because they are temperature sensitive (Noiles et al ., 1995), the idea that such changes may be induced primarily by the toxic effect of glycerol is not ruled out (Storey et al ., 1998).…”

Section: Introductionmentioning

confidence: 99%

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Glycerol decreases the integrity of the perinuclear theca in boar sperm

Núñez

Juárez-Mosqueda

Gutiérrez-Pérez

et al. 2012

Zygote

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We evaluated the effect of glycerol on the perinuclear theca (PT) of boar sperm. Samples from six ejaculates obtained from three different boars were incubated in the detergent Brij 36-T. Spermatozoa were treated with a glycerol concentration of either 2 or 4%, and incubated for 10 or 30 min; two other samples were treated with protease inhibitors (PI; leupeptin or an inhibitor commercial cocktail), mixed with 4% glycerol, and incubated for 30 min. A third glycerol-free group was used as the control. The samples were processed for electron microscopy evaluation. The PT remained intact in 78% of the control samples while, after addition of glycerol for 30 min, the proportion of spermatozoa with disrupted or absent PT increased (P < 0.05). PT was preserved in PI samples, but PT changes increased (P < 0.05). Differences due to treatment with glycerol (2 or 4%) at 10 or 30 min were not observed. These results show, to our knowledge for the first time, the adverse effect of glycerol on the integrity of the PT.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Glycerol decreases the integrity of the perinuclear theca in boar sperm

Núñez

Juárez-Mosqueda

Gutiérrez-Pérez

et al. 2012

Zygote

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“…Analysis of 27 deep-frozen ejaculates from AI bulls with the use of methods tested by various authors and standard evaluation of sperm documented that the level of the initial parameters after thawing was generally good (Rasul et al, 2001;Januskauskas et al, 2003;Martinez et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

The interrelationship between quality parameters of sperm before and after separation by gradient centrifugation

Vĕzník¹,

Švecová²,

Zajicova³

et al. 2007

Vet. Med. - Czech

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Significance of a short-time survival test for the prediction of quality parameters of sperm obtained by gradient centrifugation was evaluated from an aspect of their functional resistance. Frozen semen samples from 27 bulls before and after separation were assessed by a 120-min survival test. The evaluation of morphological examination of ejaculates was carried out using the SASMO computer program. Statistically significant interrelationship between sperm quality parameters in the initial insemination doses, the amounts of obtained sperm after separation and their functional and vital resistance in the survival test was documented.

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“…The ejaculates were then pooled, equalizing the sperm contribution of each male to eliminate individual differences (Gil et al, 2003). The tris-egg-yolk (TEY) extender used in the study comprised 60 mM citric acid, 0.25 M tris-(hydroxymethyl)-aminomethane, 69 mM fructose, 20% egg yolk, 7% glycerol in 80 mL water or ROM, 250 mg L -1 gentamycin sulfate, 50 mg L -1 tylosin tartrate, 150 mg L -1 lincomycin hydrochloride, and 300 mg L -1 spectinomycin sulfate antibiotics (Bilodeau et al, 2002;Martínez et al, 2006).…”

Section: Experimental Animals Sampling Processing and Freeze-thaw Mmentioning

confidence: 99%

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Kia

Olfati-Karaji

Hoseinkhani

et al. 2014

Span. j. agric. res.

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The present study determined the effects of the addition of rosemary extract (ROM), glutathione (GSH), and their combination (ROM + GSH) to freezing extender on the quality of bull semen after cryopreservation. Before cryoperservation, the samples were diluted in a tris-egg yolk (TEY) extender containing 5 mM GSH (treatment I), 5 or 10 g L–1 ROM (treatments II and III), and ROM with GSH (5 mM GSH with 5 or 10 g L–1 of ROM) (treatments IV and V). An extender containing no antioxidants (non-ROM/GSH-treated) served as control group. Kinematic parameters were evaluated by means of a computer-assisted semen analysis (CASA). The viability and membrane integrity of the sperm were assessed using eosin-nigrosin stain and the hypo-osmotic swelling test (HOST) at 0 and 2 h after freezethawing. Lipooxidative parameters, superoxide dismutase, and glutathione peroxidase (GPx) activity were assessed after thawing. Treatment III showed positive effects for total motility (TM) (p < 0.01), average path velocity (VAP) (p < 0.001), viability (p < 0.01) and HOST (p < 0.01); however, lipid peroxidation (LPO) decreased (p < 0.05) and GPx activity increased (p < 0.05) immediately after thawing compared to the control. The TM (p < 0.01), VAP (p < 0.01), viability (p < 0.01), HOST (p < 0.01) decreased in LPO (p < 0.01) and GPx activity (p < 0.05) for treatment V and the viability and GPx activity (p < 0.05) for treatment I were significantly higher than for the control group at 2 h after thawing. It was concluded that the inclusion of ROM and its combination with GSH improves the post-thaw quality of bull semen.

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Cryopreservation of bull spermatozoa alters the perinuclear theca

Cited by 14 publications

References 35 publications

Glycerol decreases the integrity of the perinuclear theca in boar sperm

Glycerol decreases the integrity of the perinuclear theca in boar sperm

The interrelationship between quality parameters of sperm before and after separation by gradient centrifugation

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

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