Cryopreservation of Bryophytes: The Effects of Abscisic Acid and Encapsulation Dehydration

Pence, Valerie C.

doi:10.2307/3244205

Cited by 9 publications

(11 citation statements)

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“…The most salient attribute of an IDT plant is suffering significant damage when rapidly dried, while incurring negligible damage when dried over a protracted period. In addition, IDT plants should be able to tolerate a RD event relatively undamaged following a SD event sensu Werner et al (1991) and Pence (1998). The threshold drying time (i.e., minimum rate of drying to remain relatively undamaged) for shoots of P. lamellatum , using visual shoot damage, regenerative growth rate, and chlorophyll fluorescence data, appears to be close to 24 h (between 18 and 39 h), similar to the 24‐h drying time resulting in undamaged protonemata of Funaria hygrometrica Hedw.…”

Section: Discussionmentioning

confidence: 99%

“…Hardening to DT occurs when a degree of DT is induced from either a pre‐exposure to partially dry conditions or from a slow desiccation episode, whereas dehardening (deacclimation) describes the loss of DT attending hydrated conditions and infers a degree of IDT. The ability to harden or deharden to DT is noted for several bryophytes (e.g., Abel, 1956; Dilks and Proctor, 1976; Schonbeck and Bewley, 1981a, b; Hellwege et al, 1994; Pence, 1998; Beckett, 1999; Beckett et al, 2000; Marschall and Beckett, 2005).…”

Section: Ecological Elements Of Dt In Bryophytesmentioning

confidence: 98%

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The desert moss Pterygoneurum lamellatum (Pottiaceae) exhibits an inducible ecological strategy of desiccation tolerance: Effects of rate of drying on shoot damage and regeneration

Stark

Greenwood

Brinda

et al. 2013

American J of Botany

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For the first time, a desert moss is shown to exhibit an ecological strategy of desiccation tolerance that is inducible, challenging the assumption that arid-land bryophytes rely exclusively on constitutive protection. Results indicate that previous considerations defining a slow-dry event in bryophytes need reevaluation, and that the ecological strategy of inducible desiccation tolerance is probably more common than currently understood among terrestrial bryophytes.

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Section: Discussionmentioning

confidence: 99%

Section: Ecological Elements Of Dt In Bryophytesmentioning

confidence: 98%

The desert moss Pterygoneurum lamellatum (Pottiaceae) exhibits an inducible ecological strategy of desiccation tolerance: Effects of rate of drying on shoot damage and regeneration

Stark

Greenwood

Brinda

et al. 2013

American J of Botany

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“…In mosses (Bryophyta), it has been shown that desic-cation tolerance of protonema cells of Funaria hygrometrica is increased by exogenous ABA treatment (Werner et al, 1991). Pretreatment with ABA has been shown to be effective for cryopreservation of several moss species (Pence, 1998). We previously reported that the moss Physcomitrella patens increases freezing tolerance upon ABA treatment with increased expression of stress-related genes (Minami et al, 2003(Minami et al, , 2006.…”

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confidence: 99%

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the b-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.Land plants are repeatedly challenged by environmental stresses such as desiccation, salinity, and freezing, which can cause irreversible damage to intracellular structures and membranes by severe dehydration. Abscisic acid (ABA), which is known to be a phytohormone responsible for physiological control of seed maturation and germination, bud dormancy, and stomata closure, has been postulated to be involved in the development of cellular dehydration tolerance under stress conditions. In higher plants, water-stress conditions trigger a transient or sustained increase in endogenous ABA content in tissues. Increased ABA stimulates the expression of a number of stress-related genes such as those of late embryogenesis abundant (LEA) proteins, which are thought to protect cells from the damage caused by the dehydration stress (Chandler and Robertson, 1994). It has been demonstrated that various tissue-cultured cells of vascular plants, including those of angiosperms, gymnosperms, and ferns, develop desiccation and freezing tolerance upon exogenous ABA treatment, indicating that ABA plays a key role in stress tolerance at cellular

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“… Pence (1998) described a method for the preservation of M. polymorpha thalli in liquid nitrogen. Thalli were first pre-cultured in medium containing 10 µM ABA for 1 week, cut into small pieces and encapsulated in alginate beads.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Gemmae from the LiverwortMarchantia polymorphaL.

et al. 2015

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The liverwort Marchantia polymorpha L. is one of the key model plants in evo–devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of M. polymorpha plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, thereby enabling the clonal preservation and rapid propagation of transgenic or mutant lines. Here, we report a simple cryopreservation protocol for in vitro grown M. polymorpha gemmae using aluminum cryoplates. Gemmae were pre-cultured on sucrose-containing medium, embedded in calcium alginate gel on the surface of a cryoplate, moderately dehydrated and stored in liquid nitrogen. After rapid thawing, the stored gemmae showed a 100% survival rate. Our protocol does not require plant growth regulators such as ABA, and takes only 1 h to complete except for 1 d of pre-culture. Furthermore, gemmae treated as described above but then air-dried for 2 h can be stored at −80°C for at least 1 year without a significant decrease in survival rate, which is convenient for most laboratories that have a −80°C freezer but not a liquid nitrogen container for long-term storage. These preservation techniques for M. polymorpha should increase their availability in the research community.

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Cryopreservation of Bryophytes: The Effects of Abscisic Acid and Encapsulation Dehydration

Cited by 9 publications

References 0 publications

The desert moss Pterygoneurum lamellatum (Pottiaceae) exhibits an inducible ecological strategy of desiccation tolerance: Effects of rate of drying on shoot damage and regeneration

The desert moss Pterygoneurum lamellatum (Pottiaceae) exhibits an inducible ecological strategy of desiccation tolerance: Effects of rate of drying on shoot damage and regeneration

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

Cryopreservation of Gemmae from the LiverwortMarchantia polymorphaL.

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