Cryopreservation of boar semen

Jovičić, Marija; Eva, Carola; Sedmı́ková, Markéta

doi:10.17221/47/2020-cjas

Cited by 20 publications

(28 citation statements)

References 71 publications

(97 reference statements)

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“…Inter-sire effect in the present study was observed in healthy mature animals of the same breed, in the same breeding conditions, with the semen collected and processed in the same manner. These results are supported by the previously reported inter-sire effect in livestock (Dolezalova et al 2016;Jovicic et al 2020) and in rams (D'Alessandro and Martemucci 2003;Ramon et al 2013). To this matter, the general recommendation can be to select the Wallachian rams with the best sperm viability after thawing for the preparation of insemination doses as one of the criteria in the cryopreservation program.…”

Section: Inter-sire and Daily Variation Impacted The Viability Of Thawed Wallachian Ram Spermsupporting

confidence: 77%

“…Such equilibration procedure was reported as a usual procedure for ram sperm (Lv et al 2019). Nevertheless, there exists a recommendation to add glycerol immediately just before the sperm freezing (Jovicic et al 2020). This recommendation might be well-reasoned because of the known glycerol toxicity, as Domingo et al (2019) reviewed.…”

Section: Cryopreservation Decreases the Viability Of Wallachian Ram Spermmentioning

confidence: 99%

“…From the practical point of view, it is interesting to apply this well-established, simple freezing method in the Wallachian sheep conservation program to cryopreserve Wallachian ram sperm. However, considering previously reported interbreed (Beran et al 2011;Olah et al 2013), and even inter-sire (Ramon et al 2013;Dolezalova et al 2016;Jovicic et al 2020) differences in livestock sperm freezability, we are aware of the fact that the freezing method must first be thoroughly optimized to use with the specific breed.…”

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confidence: 99%

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Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program

Savvulidi¹,

Ptáček²,

Málková³

et al. 2021

Czech J. Anim. Sci.

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The aim of the present study was to optimize the conventional method of sperm freezing in liquid nitrogen (LN2) vapour for successful cryopreservation of Wallachian ram sperm, the genetic resources of the Czech Republic. Sperm in straws were frozen using the conventional freezing method via a static exposure of sperm doses to LN2 vapour, or by four different modified freezing methods. Under modified freezing, straws were frozen by a discontinuous, time-dependent decremental change in the distance between the straws and the surface of LN2. The viability of sperm was evaluated by flow cytometry after sperm equilibration, and immediately after thawing. Besides the observed inter-sire and daily variation, the obtained results suggest the methodological weakness of the conventional freezing method via the static exposure of sperm doses to LN2 vapour. With the use of the optimized freezing procedure, all parameters of thawed sperm were significantly (P < 0.05) improved in comparison with the conventional method: percentage of thawed sperm viability increased up to 48.3%, percentage of sperm with plasma membrane damage after thawing decreased to 6.58%, percentage of sperm with acrosome damage decreased to 24.4%, and percentage of sperm with deteriorated mitochondrial activity decreased to 6.28%. In conclusion, our results suggest that an optimized freezing procedure should be routinely used instead of the conventional method to cryopreserve Wallachian ram sperm.

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Section: Inter-sire and Daily Variation Impacted The Viability Of Thawed Wallachian Ram Spermsupporting

confidence: 77%

Section: Cryopreservation Decreases the Viability Of Wallachian Ram Spermmentioning

confidence: 99%

mentioning

confidence: 99%

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Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program

Savvulidi¹,

Ptáček²,

Málková³

et al. 2021

Czech J. Anim. Sci.

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“…Our results are consistent with studies of red deer (Ramon et al 2012) andboars (Flores et al 2009), in which the males with poor freezability were characterised by a high percentage of the sperm subpopulation with low kinematic parameters. Cryopreservation in boars is a long-term challenge (Jovicic et al 2020), similarly like in stallions, because the sperm are highly sensitive to cold shock and both species have a high proportion of seminal plasma in the semen; studies conducted on boar sperm may therefore provide a comparison for our results in stallions. The mitochondrial activity was also lowest in poor freezer boars, and in addition, Hernandez et al (2006) identified less homogeneous chromatin in PF boars.…”

Section: Incubation Time T30mentioning

confidence: 99%

The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers

Bubeníčková¹,

Šichtař²,

Nováčková³

et al. 2020

CZECH J ANIM SCI

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The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm (n = 57 630) into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30 (P < 0.05). Spermatozoa of good freezers are represented more frequently in the fast and medium fast subpopulations and are faster and more linear than those of poor freezers (P < 0.05). Sperm from PF stallions were more strongly affected by longer incubation. The percentage of sperm in the fast and medium fast subpopulations was lower in samples from PF stallions, but assessment of the motility parameters in particular sperm subpopulations revealed that these sperm had good velocity. Poor freezer samples had lower sperm quality due to a reduced total proportion of motile sperm, and these samples were more sensitive to prolonged time after thawing. Thus, an efficient sperm selection method or a special insemination technique should be used for obtaining doses from stallions with poor freezability. Our study showed that the CASA system and cluster analysis are promising tools for better understanding the significant differences in the individual stallion freezability, and further research should be focused on their application in the field.

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“…Pig arti cial insemination (AI) has been widely used in recent years, and the sperm storage at 17℃ has important signi cance in commercial production [8,22]. Pig sperm is extremely sensitive to temperature changes especially low temperature [15,32]. The cold shock caused by low temperature has a serious impact on sperm quality and the fertilization potential of sperm [13,25,31].…”

Section: Introductionmentioning

confidence: 99%

Hydroxytyrosol Effectively Improves The Quality of Pig Sperm at 17 °C

Liu

Zhang

Tian

et al. 2021

Preprint

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Background: Artificial insemination technology is the production technology used in more than 99% of the world's pig farms. As the frozen sperm damage is very serious, the preservation of pig sperm is mainly carried out at 17°C. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen species and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol group (optimum concentration). Ultimately, we selected the control group and the 120 μmol/L hydroxytyrosol group to test the effect of artificial insemination.Results: The results of our research showed that during storage of pig sperm at 17 ℃, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated group were significantly improved (P < 0.05). Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm quality. Besides, the significant increase in sow pregnancy rate and piglet birth weight also confirmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrate that the addition of hydroxytyrosol to the diluent can improve the quality of pig sperm and the efficiency of artificial insemination.

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Cryopreservation of boar semen

Cited by 20 publications

References 71 publications

Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program

Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program

The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers

Hydroxytyrosol Effectively Improves The Quality of Pig Sperm at 17 °C

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