Cryopreservation of boar semen and its future importance to the industry

Bailey, Janice L.; Lessard, Christian; Jacques, Joannie; Bréque, Christelle; Dobrinski, Ina; Zeng, Wenxian; Galantino‐Homer, Hannah

doi:10.1016/j.theriogenology.2008.06.014

Cited by 124 publications

(97 citation statements)

References 56 publications

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“…Perhaps, both are good general measures of the function of the cell surface and, for that reason, only one of these apparently redundant attributes was necessary in the model to predict fertility. Although there is less information on the effect of cryopreservation on sperm binding to the zona pellucida, it is known that cryopreservation induces precocious capacitation of boar spermatozoa and/or acrosome damage (Bailey et al, 2008) and reduces the ability of spermatozoa to bind oviduct epithelial cells (Lefebvre & Suarez, 1996;Fazeli et al, 1999). This reduction is probably because premature capacitation and acrosomal damage can reduce oviduct cell and zona pellucida binding (Ivanova & Mollova, 1993;Bailey et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Although there is less information on the effect of cryopreservation on sperm binding to the zona pellucida, it is known that cryopreservation induces precocious capacitation of boar spermatozoa and/or acrosome damage (Bailey et al, 2008) and reduces the ability of spermatozoa to bind oviduct epithelial cells (Lefebvre & Suarez, 1996;Fazeli et al, 1999). This reduction is probably because premature capacitation and acrosomal damage can reduce oviduct cell and zona pellucida binding (Ivanova & Mollova, 1993;Bailey et al, 2008). The final model included an inverse relationship between the percentage of acrosome-compromised spermatozoa after 30 min (T30) and percentage of piglets sired by a specific boar (Table 1) but, in contrast, a positive relationship between acrosome-compromised spermatozoa at T0 and fertility (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Various routine and novel assays were tested for their relationship to fertility as assessed by competitive insemination of gilts. In addition to traditional post-thaw analyses of sperm function (motility, viability, and acrosome integrity), we assessed oviduct binding because cryopreservation may cause precocious capacitation and hinder the ability of spermatozoa to bind to the oviduct epithelium and form a reservoir (Lefebvre & Suarez, 1996;Fazeli et al, 1999;Bailey et al, 2008). A competitive zona-binding assay using two different fluorophores for boar identification was employed to detect differences in the number of sperm bound to the zona of oocytes, a potential indicator of sperm damage.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Daigneault

McNamara

Purdy³

et al. 2015

Andrology

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SUMMARYDue to reduced fertility, cryopreserved semen is seldom used for commercial porcine artificial insemination (AI). Predicting the fertility of individual frozen ejaculates for selection of higher quality semen prior to AI would increase overall success. Our objective was to test novel and traditional laboratory analyses to identify characteristics of cryopreserved spermatozoa that are related to boar fertility. Traditional post-thaw analyses of motility, viability, and acrosome integrity were performed on each ejaculate. In vitro fertilization, cleavage, and blastocyst development were also determined. Finally, spermatozoa-oviduct binding and competitive zonabinding assays were applied to assess sperm adhesion to these two matrices. Fertility of the same ejaculates subjected to laboratory assays was determined for each boar by multi-sire AI and defined as (i) the mean percentage of the litter sired and (ii) the mean number of piglets sired in each litter. Means of each laboratory evaluation were calculated for each boar and those values were applied to multiple linear regression analyses to determine which sperm traits could collectively estimate fertility in the simplest model. The regression model to predict the percent of litter sired by each boar was highly effective (p < 0.001, r 2 = 0.87) and included five traits;acrosome-compromised spermatozoa, percent live spermatozoa (0 and 60 min post-thaw), percent total motility, and the number of zona-bound spermatozoa. A second model to predict the number of piglets sired by boar was also effective (p < 0.05, r 2 = 0.57).These models indicate that the fertility of cryopreserved boar spermatozoa can be predicted effectively by including traditional and novel laboratory assays that consider functions of spermatozoa.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Daigneault

McNamara

Purdy³

et al. 2015

Andrology

View full text Add to dashboard Cite

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“…Iulian IBĂNESCU 1,2* , Petru ROŞCA 1 , Ioana CRIVEI 1 preservation in boar, maintaining higher sperm fertility compared with freezing method (Hu et al, 2015).…”

Section: Correlations Between Some Parameters Of Raw and Stored Semenmentioning

confidence: 99%

Correlations between some Parameters of Raw and Stored Semen in Boar

et al. 2015

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The preservation in liquid form, at 17°C for several days is still the most used method of semen preservation in boar. In these conditions, it would be of great value to know the factors that can predict the capacity of an ejaculate to maintain its biological value over preservation period. This study aimed to identify the correlations between some parameters of raw semen, and those of extended semen, stored in liquid form, for three days at 17°C. Over 1000 ejaculates were collected by means of manual method and artificial vagina over a period of two years from 400 mature boars housed in a specialized unit in Germany. The semen was examined immediately after collection (before dilution) as well as after three days of preservation. All the parameters (except the volume) were determined by means of computerized instruments. The examination of raw semen was performed using SQA-Vp, while the stored semen was analyzed using Spermvision version 3.7. All data were processed with IBM SPSS Statistics version 21. The Pearson correlation revealed no strong correlation between any of the raw semen parameters when analysed together with stored semen parameters. However, some moderate or weak correlations were found, for example a positive correlation between motility of raw semen and motility of stored semen (p<0.01), a positive correlation between percentage of morphological normal sperm in raw semen and the motility of stored semen (p<0.01), a negative correlation between the concentration of raw semen and the linearity and wobble coefficient in stored semen (p<0.01) as well as a couple other weak correlations. We conclude that, despite the fact that some correlations were revealed, unfortunately the analyzed parameters of raw semen have low predictive value on the parameters of extended semen after preservation at 17°C, and that the parameters of semen will behave differently from one ejaculate to another.

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“…This is due to the fact that cryopreservation of semen allows both the storage of genetic material for a long time, and the introduction of desired traits to the currently run breeding lines (Johnson et al 2000, Gerrits et al 2005, Bailey et al 2008). In addition, it contributes to the prevention of possible biological hazards in the production of high quality pork, and allows the reproduction of parents' genetic traits eliminated during an epidemic (Bailey et al 2008, Didion et al 2013. Therefore, in this aspect, cryopreservation of semen is of great significance in the long-term storage of genetic material.…”

Section: Introductionmentioning

confidence: 99%

Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa

Dziekońska¹,

Zasiadczyk²,

Lecewicz³

et al. 2015

Polish Journal of Veterinary Sciences

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The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17 o C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridge (NAR) acrosome, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), measurements of ATP content and activity of superoxidase dismutase (SOD) and glutathione peroxidase (GPx). Prior to the freezing process, no significant effect of the extender on the sperm quality parameters was noted. After thawing the spermatozoa it was demonstrated that the type of extender used influenced PMI, MMP, ATP content and activity of GPx. In the AH extender the percentage of spermatozoa with PMI and ATP content in spermatozoa was significantly higher (P<0.05) as compared to the BTS or GD extenders. In addition, semen stored in the AH was characterised by a statistically higher (P<0.05) percentage of spermatozoa with MMP and increased activity of GPx as compared with the BTS. The results obtained indicate that for the cryopreservation process, boar spermatozoa stored for 24 hours in liquid state can be used. However, the type of extender used prior to freezing may have a significant effect on the post-thawing quality of the spermatozoa. The AH extender better secured the quality of thawed boar spermatozoa as compared with the BTS or GD.

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Cryopreservation of boar semen and its future importance to the industry

Cited by 124 publications

References 56 publications

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Correlations between some Parameters of Raw and Stored Semen in Boar

Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa

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