2023
DOI: 10.1016/j.theriogenology.2023.07.028
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Cryopreservation of black seabream (Acanthopagrus schlegelii) sperm

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Cited by 5 publications
(3 citation statements)
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“…Liquid nitrogen fumigation and program-controlled cryostat freezing are the primary methods used for semen freezing currently. For most laboratories dealing with non-industrialized species and production, liquid nitrogen fumigation remains the primary freezing method 22 , 23 . The cooling rate of liquid nitrogen fumigation is determined by the height from the liquid nitrogen surface.…”
Section: Discussionmentioning
confidence: 99%
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“…Liquid nitrogen fumigation and program-controlled cryostat freezing are the primary methods used for semen freezing currently. For most laboratories dealing with non-industrialized species and production, liquid nitrogen fumigation remains the primary freezing method 22 , 23 . The cooling rate of liquid nitrogen fumigation is determined by the height from the liquid nitrogen surface.…”
Section: Discussionmentioning
confidence: 99%
“…For most laboratories dealing with non-industrialized species and production, liquid nitrogen fumigation remains the primary freezing method 22,23 . The cooling rate of liquid nitrogen fumigation is determined by the In semen freezing, even though antifreeze is added to the diluent, it may still be affected by dangerous temperature ranges 24 .…”
Section: Discussionmentioning
confidence: 99%
“…The MAS medium itself (without any CPA) was able to produce better viability values than other tested media even supplemented with CPAs, suggesting that the MAS composition counts with essential components for the conservation of the cells. Concretely, the MAS medium contains glucose, which provides the energy for basic maintenance and cell metabolism during the cryopreservation period and, as happened with other sugars, provides antioxidant protection (Pan et al, 2023;Whaley et al, 2021;Anjos et al, 2021); trisodium citrate, which supplements the medium with mineral salts providing a suitable ionic environment that mimics the natural extracellular environment, maintaining the osmolarity and ionic balance of the cells while acting as a buffer, keeping the pH stable during the freezing and thawing process and thus protecting the cells from sudden changes in acidity or alkalinity that could damage them (Herrler et al, 2006). Moreover, this molecule also can act as a scavenger by binding to metal ions such as calcium and magnesium, which helps to prevent the formation of precipitates and stabilize solutions (Pham and Schwartz; 2013).…”
Section: Discussionmentioning
confidence: 99%