Cryopreservation of articular cartilage. Part 3: The liquidus-tracking method

Pegg, D.E.; Wang, Lihong; Vaughan, D. H.

doi:10.1016/j.cryobiol.2006.01.004

Cited by 74 publications

(40 citation statements)

References 15 publications

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“…The temperatures and concentrations of the bathing solution for both protocols follow Pegg et al (2006c)'s values. In Protocol I, as did Pegg et al (2006c), the duration for each step is set to be 30 min except for two steps at 22 °C, while in Protocol II, the duration for each subzero step is varied so that the T m,c [melting point of tissue calculated by the final center concentration of current step using the published equation (Pegg, 1986)] at the end of current step is 1 °C lower than the exposure temperature of the next step. The initial concentration in AC is zero and the objective is to increase the center concentration (mass fraction) to 47.6%, the minimal concentration for vitrification (Hua and Ren, 1994).…”

Section: Resultsmentioning

confidence: 99%

“…This type of vitrification of AC has been studied by several research groups (Song et al, 2004a;2004b;Brockbank et al, 2010). Recently, as an alternative, Pegg et al (2006c) proposed a novel approach called "liquidustracking" (LT) method to vitrify cartilage samples. The ability of post-thawing cartilage specimens to incorporate sulfate ( 35 S) into newly synthesized glycosaminoglycans (GAGs) was found to approach 70% of that of fresh control groups.…”

Section: Introductionmentioning

confidence: 99%

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Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Zhang

Chen

2012

J. Zhejiang Univ. Sci. B

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Abstract:Osteochondral allografting has been proved to be a useful method to treat diseased or damaged areas of joint surfaces. Operational long-term stocks of grafts which supply a buffer between procurement and utilization would contribute to the commercialization or industrialization of this technology. Vitrification has been thought to be a promising method for successful preservation of articular cartilage (AC), but high concentration cryoprotectants (CPAs) are used which may cause high cellular toxicity. An effective way to reduce CPA toxicity is to increase CPA concentration gradually while the temperature is lowered. Understanding the mechanism of CPA permeation at subzero temperatures is important for designing the cryopreservation protocol. In this research, the permeation of dimethyl sulfoxide (Me 2 SO) in ovine AC at subzero temperatures was studied experimentally. Pretreated AC discs were exposed in Me 2 SO solutions for different time (0, 5, 15, 30, 50, 80, and 120 min) at three temperature levels (−10, −20, and −30 °C). The Me 2 SO concentration within the tissue was determined by ultraviolet (UV) spectrophotometry. The diffusion coefficients were estimated to be 0.85×10 −6 , 0.48×10 −6 , and 0.27×10 −6 cm 2 /s at −10, −20, and −30 °C, respectively, and the corresponding activation energy was 29.23 kJ/mol. Numerical simulation was performed to compare two Me 2 SO addition protocols, and the results demonstrated that the total loading duration could be effectively reduced with the knowledge of permeation kinetics.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Zhang

Chen

2012

J. Zhejiang Univ. Sci. B

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“…Though reduction of steps increased the concentration gradient, and thus, osmotic imbalances, our results show that less steps produced no negative effects upon cell viability levels. Pegg et al [29] found that chondrocytes can withstand osmotic imbalances far better than other cells. The higher concentration gradient in the 4/4 step method, however, may have underexposed the TECCs to the cryoprotectants.…”

Section: Discussionmentioning

confidence: 99%

Protocol Development for Vitrification of Tissue-Engineered Cartilage

Farooque

Chen²,

Schwartz³

et al. 2010

BioProcess J

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“…Therefore, preservation at 4°C, although sub-optimal, is used in commercial tissue banks. However, a recent report described newly-developed methods of cartilage cryopreservation which reduced cell damage associated with ice crystal formation and this method has been applied to cartilage cryopreservation in some tissue banks (Pegg et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Cold preservation of rat osteochondral tissues in two types of solid organ preservation solution, culture medium and saline

et al. 2008

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We compared Dulbecco's modified Eagle's medium (DMEM), saline, Euro-Collins (EC) solution and University of Wisconsin (UW) solution to determine which was best for cold preservation of rat osteochondral tissues (OCTs). After 7 days' cold preservation, OCTs kept in UW solution had the highest relative viable cell number by the tetrazolium assay and the lowest activity of lactate dehydrogenase released from damaged cells. Histological evaluation revealed chondrocyte deformity, such as shrunken cytoplasm and pyknotic nuclei, particularly in the deeper layer of articular cartilage after preservation in saline and EC solution and predominantly in all layers if preserved in DMEM. In contrast, chondrocyte morphology in all layers of the articular cartilage preserved in UW solution was relatively unchanged and remained similar to fresh OCTs. It is therefore concluded that UW solution is the most suitable for cold preservation of rat OCTs as well as solid organs.

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Cryopreservation of articular cartilage. Part 3: The liquidus-tracking method

Cited by 74 publications

References 15 publications

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Protocol Development for Vitrification of Tissue-Engineered Cartilage

Cold preservation of rat osteochondral tissues in two types of solid organ preservation solution, culture medium and saline

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