Cryopreservation of animal oocytes and embryos: Current progress and future prospects

Mandawala, A.A.; Harvey, Simon C.; Roy, Tammie K.; Fowler, Katie E.

doi:10.1016/j.theriogenology.2016.07.018

Cited by 126 publications

(75 citation statements)

References 78 publications

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“…Many reports support the idea that pig oocytes are more sensitive than mouse oocytes to physiological stress such as cooling and warming [11, 36, 37]. It was suggested that many lipids exist in the cytoplasm of pig oocytes, which negatively affect developmental ability after thawing [38].…”

Section: Discussionmentioning

confidence: 99%

Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated ε-poly-L-lysine

et al. 2017

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Vitrification is a powerful tool for the efficient production of offspring derived from cryopreserved oocytes or embryos in mammalian species including domestic animals. Genome editing technologies such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas)9 are now available even for domestic species, suggesting that the vitrification of embryos at the pronuclear stage (PN) will be more important because they could provide genomic host cells to be targeted by TALENs or CRISPR/Cas9. Although we reported the successful production of piglets derived from vitrified PN embryos by a solid-surface vitrification method with glutathione supplementation, further improvements are required. The cryoprotective agent (CPA) carboxylated ε-poly-L-lysine (COOH-PLL) was introduced in 2009. COOH-PLL reduces the physical and physiological damage caused by cryopreservation in mammalian stem cells and the vitrification of mouse oocytes and embryos. Those results suggested that vitrification of COOH-PLL may help improve the developmental ability of pig embryos vitrified at the PN stage. However, it remains unclear whether COOH-PLL is available as a CPA for the vitrification of embryos in domestic species. In this study, we evaluated COOH-PLL as a CPA with ethylene glycol (EG) and Cryotop as a device for the vitrification of PN pig embryos. Exposure to vitrification solution supplemented with COOH-PLL up to 30% did not decrease developmental ability to the 2-cell stage and the blastocyst stage. After warming, most of the vitrified embryos survived regardless of the concentration of COOH-PLL (76.0 ± 11.8% to 91.8 ± 4.6%). However, the vitrified embryos without COOH-PLL showed a lower development rate up to the blastocyst stage (1.3 ± 1.0%) compared to the fresh embryos (28.4 ± 5.0%) (p<0.05). In contrast, supplementation of 20% (w/v) COOH-PLL in the vitrification solution dramatically improved the developmental ability to blastocysts of the vitrified embryos (19.4 ± 4.6%) compared to those without COOH-PLL (p<0.05). After the transfer of embryos vitrified with 30% (v/v) EG and 20% (w/v) COOH-PLL, we successfully obtained 15 piglets from 8 recipients. Taken together, our present findings demonstrate for the first time that COOH-PLL is an effective CPA for embryo vitrification in the pig. COOH-PLL is a promising CPA for further improvements in the vitrification of oocytes and embryos in mammalian species.

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Section: Discussionmentioning

confidence: 99%

Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated ε-poly-L-lysine

et al. 2017

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“…Slow freezing first substitutes the water within the cytoplasm with CPAs which reduces cell damage and adjusts the cooling rate in accordance with the permeability of the cell membrane. Slow-cooling protocols involve a typical cooling rate of about 1 °C/min in the presence of less than 1.0M of CPA, with use of a high-cost controlled-rate freezer or a benchtop portable freezing container 8, 9. The advantages of slow freezing are that it has a low risk of contamination during the procedures and does not demand high manipulation skills.…”

Section: Freezing Method: Conventional Slow Freezing and Vitrificationmentioning

confidence: 99%

“…1 and Table 1). 5 Cryopreservation processes can generally be grouped into the following types: (1) slow freezing8, 9; (2) vitrification, which involves the solidification of the aqueous milieu of the cell or tissue into a noncrystalline glassy phase 10 ; (3) subzero nonfreezing storage; and (4) preservation in the dry state 11 . Generally, the storage of mammalian cells in the dry state is not readily possible because of difficulties in introducing the disaccharide trehalose (disaccharide of glucose, 342 Da) 12 and amino acids (used as preservatives in plants) into the intracellular region 13 .…”

Section: Cryopreservationmentioning

confidence: 99%

“…The applications of cryopreservation (Table 2) can be categorized into the following areas: (1) cryopreservation of cells or organs 5 ; (2) cryosurgery; (3) biochemistry and molecular biology; (4) food sciences; (5) ecology and plant physiology; and (6) many medical applications, such as blood transfusion, bone marrow transplantation, artificial insemination, and in vitro fertilization (IVF) 3, 5, 8, 11, 36, 37, 38, 39. Some suggested advantages of cryopreservation include the possible banking of cells for human leukocyte antigen typing for organ transplantation, the allowance of sufficient time for transport of cells and tissues among different medical centers, and the provision of research sources for identifying unknown transmissible diseases or pathogens 5 .…”

Section: Applications Of Cryopreservationmentioning

confidence: 99%

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Cryopreservation and its clinical applications

Jang

Park

Yang

et al. 2017

Integrative Medicine Research

340

234

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Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures. The responses of living cells to ice formation are of theoretical interest and practical relevance. Stem cells and other viable tissues, which have great potential for use in basic research as well as for many medical applications, cannot be stored with simple cooling or freezing for a long time because ice crystal formation, osmotic shock, and membrane damage during freezing and thawing will cause cell death. The successful cryopreservation of cells and tissues has been gradually increasing in recent years, with the use of cryoprotective agents and temperature control equipment. Continuous understanding of the physical and chemical properties that occur in the freezing and thawing cycle will be necessary for the successful cryopreservation of cells or tissues and their clinical applications. In this review, we briefly address representative cryopreservation processes, such as slow freezing and vitrification, and the available cryoprotective agents. In addition, some adverse effects of cryopreservation are mentioned.

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“…Cryopreservation has been extensively studied as a viable solution to the long-term storage of various biomaterials, such as oocytes [15], stem cells [16, 17], vascular tissues [18], and embryos [19]. Recently, several groups demonstrated the feasibility of obtaining viable PDLSCs from frozen periodontal ligament tissues or intact whole teeth [20, 21].…”

Section: Introductionmentioning

confidence: 99%

Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheets

Li¹,

Feng²,

Gu³

et al. 2017

Stem Cell Res Ther

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BackgroundCryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets.MethodsPDLSC sheets prepared from extracted human molars were divided into two groups: the cryopreservation group (cPDLSC sheets) and the freshly prepared control group (fPDLSC sheets). The cPDLSC sheets were cryopreserved in a solution consisting of 90% fetal bovine serum and 10% dimethyl sulfoxide for 3 months. Cell viability and cell proliferation rates of PDLSCs in both groups were evaluated by cell viability assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, respectively. The multilineage differentiation potentials of the cells were assessed by von Kossa staining and Oil Red O staining. The chromosomal stability was examined by karyotype analysis. Moreover, the cell sheets in each group were transplanted subcutaneously into the dorsal site of nude mice, after which Sirius Red staining was performed to analyze the efficiency of tissue regeneration.ResultsThe PDLSCs derived from both groups of cell sheets showed no significant difference in their viability, proliferative capacities, and multilineage differentiation potentials, as well as chromosomal stability. Furthermore, transplantation experiments based on a mouse model demonstrated that the cPDLSC sheets were equally effective in generating viable osteoid tissues in vivo as their freshly prepared counterparts. In both cases, the regenerated tissues showed similar network patterns of bone-like matrix.ConclusionsOur results offer convincing evidence that cryopreservation does not alter the biological properties of PDLSC sheets and could enhance their clinical utility in tissue regeneration.

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Cryopreservation of animal oocytes and embryos: Current progress and future prospects

Cited by 126 publications

References 78 publications

Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated ε-poly-L-lysine

Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated ε-poly-L-lysine

Cryopreservation and its clinical applications

Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheets

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