Cryopreservation of Andalusian donkey (Equus asinus) spermatozoa: Use of alternative energy sources in the freezing extender affects post-thaw sperm motility patterns but not DNA stability

Dorado, J.; Hidalgo, M.; Acha, D.; Ortiz, Isabel; Bottrel, M.; Azcona, F.; Carrasco, J.J.; Gómez-Arrones, V.; Demyda-Peyrás, S.

doi:10.1016/j.anireprosci.2019.106126

Cited by 7 publications

(4 citation statements)

References 57 publications

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“…As well as, sugars prevent cooling damage of sperm by affecting the pattern of ice-crystal formation and the width of the channels of unfrozen extender (Nicolajsen and Hvidt, 1994) and reduce the salt concentration of the extender that can reduce the injury due to the extender solution effects. Recent studies were indicated that spermatozoa motility depends greatly on the energy source in an extender in the form of adenosine triphosphate (ATP), which is produced from metabolism, and therefore requires a constant supply for cell function and survival (Dorado et al, 2019). This study result finding broadly supports the work of other studies in this area linking motility with source of energy and production of ATP which our result showed increased significantly in the general and progressive motility in addition of aqueoussemen extender as showed in Figure (1 and 2).…”

Section: Discussionmentioning

confidence: 99%

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

AbdUlAmeer¹,

Zaid²

2022

ijhs

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This study was designed in order to investigate and determine the effect of black current (Prunus cerasus) extract on semen general and progressive motility of the rams. Twelve mature healthy rams was used in this study aged between 2-3 years, kept in the animal farm of the College of Veterinary Medicine, Al-Kufa University. Semen ejaculates was collected using electroejaculator methods once weekly a total of 36 ejaculates were collected among the period of the study. The black current (Prunus cerasus) extract was prepared by using maceration and added to the semen in five doses groups (0.25 gm/ml as G1, 0.5 gm/ml as G2, 1 gm/ml as G3, 2 gm/ml as G4, 4 gm/ml as G5) and the positive control group left without any addition. The treatment samples were evaluated under cooling for five consecutive days, the general and progressive motility were analysis using Computer Assisted Sperm Analysis (CASA). The results of the study clearly indicated that there were a significant differences (P<0.05) in G1 and G2 than other groups in five days of examination, with the preference to the G1 group.

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Section: Discussionmentioning

confidence: 99%

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

AbdUlAmeer¹,

Zaid²

2022

ijhs

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“…As expected, post-thawed motility parameters and the FI of motility were significantly higher when using the Botucrio extender (p < 0.05). This extender contains amino acids, carbohydrates, and N-methylformamide, which improve sperm post-thawed motility (20,22,50,54). Further studies are necessary to verify if changes in component concentrations (glycerol and egg yolk) and new additives (amides, carbohydrates and amino acids) on HM-0 formulation improves the freezability index (viability and sperm motility) of donkey sperm using a cryopreservation extender based on Trisglycerol-egg yolk.…”

Section: Discussionmentioning

confidence: 99%

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

et al. 2021

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The Baudet du Poitou is a vanishing donkey breed recognized for engendering robust working mules. In Chile, only two pure breed Poitou males exist, which belong to the Chilean army and are used for mule production. We performed an extensive sperm and seminal analysis of these two jackasses aged 3 and 6 years and investigated the use of a simple hypometabolic extender for sperm cryopreservation. Computer-assisted sperm analysis showed high motility, velocity, and linearity in sperm movement. The seminal plasma analysis revealed that sodium and chloride were the main electrolytes, and globulins were the main metabolites. Active and variable enzymatic activity was observed. New information is reported about gamma-glutamyltransferase, aspartate aminotransferase, zinc, and magnesium concentrations in seminal plasma of Poitou donkeys. Ejaculates among jackasses showed some variability due to individual variability and different stages in sexual maturation according to age. The freezability index analysis based in viability, total motility and progressive motility with Botucrio extender (57.1 ± 11.0%; 56.6 ± 20.0%; and 22.6 ± 10.3%, respectively) were significantly higher (p < 0.05, p < 0.0001, and p < 0.0001, respectively) than with HM-0 extender (42,6 ± 11.4%; 14.9 ± 5.1%; and 1.0 ± 2.5%, respectively). We report new information on Poitou donkey semen and cryopreservation in the Southern Hemisphere that could be useful in donkey breeding and conservation programs to develop strategies that improve the effectiveness of population management of this breed.

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“…Hussain et al, J. Dorado et al, C.-H. Liu et al, and A. Ghaniei et al have confirmed that using honey as an extender in cryopreservation improved the semen quality and acted as natural antibiotics against pathogenic bacteria. Moreover, it resolved many challenges, such as freezing solution toxicity, pH irregularity, ROS, energy source, damage of sperm membrane, and cryoshock preservatives [149][150][151][152].…”

Section: Extendersmentioning

confidence: 99%

The Natural Cryoprotectant Honey for Fertility Cryopreservation

2022

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Honey is a mixture of 25 sugars with other bioactive substances (i.e., organic acids, enzymes, antioxidants, and vitamins) and has been known as a highly nutritious functional food. Traditionally, it has been widely used in medicinal applications to cure various diseases. The effectiveness of honey in different applications has been used for its antimicrobial activity, absorption of hydrops, cleansing, removing odor, assisting granulation, recovery of nutrition, and formation of tissue and epithelium, which proved that honey has dehydrating and preserving properties to make it ideal for the cryopreservation of cells and tissues. Cryopreservation is an advanced preservation technique for tissue, cells, organelles, or other biological specimen storage, performed by cooling the sample at a very low temperature. It is the most common approach to improved preserving fertility (sperm, embryos, and oocytes) in different species that may undergo various life-threatening illnesses and allows for the genetic screening of these cells to test the sample for diseases before use. However, with toxic cryoprotectant (CPA), cryopreservation of fertility has been challenging because of their particular structure and sensitivity to chilling. Honey’s unique composition, as well as its dehydrating and preserving properties, qualify it to be used as a natural cryoprotectant. The aim of this study is to emphasize the ability of honey as a natural cryoprotectant in cryopreservation. The articles for this review were searched from Google Scholar, PubMed, Science Direct, Web of Science, and Scopus, using the keywords, honey, cryopreservation, natural cryoprotectant/CPAs, extenders, and fertility. Honey, as a natural cryoprotectant in fertility cryopreservation, yielded satisfactory results, with respect to improved post-thaw quality and viability. It is now proved as a non-toxic and highly efficient natural cryoprotectant in fertility preservation because its increasing viscosity at low temperature can provide a protective barrier to cells by reducing ice formation. Furthermore, its antioxidant property plays a vital role in protecting the cells from thermal damage by reducing the reactive oxygen species (ROS). This review provides a road map for future studies to investigate the potential of honey in the cryopreservation of other cells and tissue and contribute to the scientific research, regarding this remarkable natural product.

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Cryopreservation of Andalusian donkey (Equus asinus) spermatozoa: Use of alternative energy sources in the freezing extender affects post-thaw sperm motility patterns but not DNA stability

Cited by 7 publications

References 57 publications

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

General and Progressive Semen Motility Affected by using Black Currant (Prunus cerasus) Extracts in Rams

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

The Natural Cryoprotectant Honey for Fertility Cryopreservation

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