Cryopreservation of an embryogenic suspension culture of <i>Picea sitchensis</i> and subsequent plant regeneration

Floto, F.; Krogstrup, Peter; M⊘ller, Jette Dahl; N�rgaard, J. V.; Kristensen, Michel M. H.

doi:10.1080/02827589309382765

Cited by 28 publications

(13 citation statements)

References 13 publications

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“…Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve the uniformity and quality of the nursery stock, particularly in conifer species (Find et al 1993). Since first reported for spruce (Hakman et al 1985), SE is favored as a promising tool for mass propagation of coniferous trees (Park 2002).…”

mentioning

confidence: 99%

Embryogenic tissue initiation and somatic embryogenesis in Fraser fir (Abies fraseir [Pursh] Poir.)

Kim

Newton

Frampton

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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Embryogenic suspensor mass (ESM) was established from immature seeds of Fraser fir. The initiation frequency of ESM was dependent on genotype, collection time, medium, and plant growth regulators (PGR) used. The ESM initiation potential was higher with seeds collected in late June (clone 16-273, 4.7%) or early July (clone 16-45, 2.2%) and decreased as the zygotic embryos matured. Excised proembryo stage of zygotic embryos was most appropriate to initiation of ESM. Most of the ESM arose from the seeds that were at the proembryo stage. From the four different culture media we compared, seven ESM lines were obtained: two lines from Murashige and Skoog (MS) medium with 4.4 μM benzyladenine (BA), one from Schenk and Hildebrandt (SH) medium with 4.5 μM thidiazuron (TDZ), and four from SH with 4.4 μM 6-benzyladenine. However, only one ESM line from clone 16-273 (June 24, SH+TDZ) could be proliferated in subsequent culture. Different concentrations of L-glutamine and casein hydrolysate (CH) in the medium were also compared for their effect on ESM proliferation. The highest proliferation rate (1.16-fold) was obtained from SH medium supplemented with 250 mg/L CH and 3.42 mM L-glutamine. In contrast, the lowest rate was noted when 1,000 mg/L CH plus 3.42 mM L-glutamine (0.17-fold) was added to the medium. As for somatic embryo maturation, the highest number of mature precotyledonary (100.1/g −1 FW ESM) or cotyledonary (64.3/g −1 FW ESM) somatic embryos was obtained on a medium containing 20 or 80 μM abscisic acid, 10% polyethyleneglycol, 4% maltose, and 0.3% gellan gum. For germination of the somatic embryos, the cotyledonary somatic embryos derived from maturation medium were transferred on halfstrength Litvay medium containing 0.3% gellan gum. The somatic plantlets were recovered from the germination medium and transferred to soils.

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confidence: 99%

Embryogenic tissue initiation and somatic embryogenesis in Fraser fir (Abies fraseir [Pursh] Poir.)

Kim

Newton

Frampton

et al. 2008

In Vitro Cell.Dev.Biol.-Plant

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“…Aliquots of 1 ml of suspended tissue were then transferred to 2 ml cryovials (Nunc) and kept on ice until being transferred to a programmable freezer (Model 7452 Series CryoMed Controlled Rate Freezer, Thermo Scientific). The programme used was based on Find et al (1993), with a cooling rate of 0.5 °C min −1 to − 17.5 °C where it was held for 10 min, then 0.5 °C min −1 to − 40 °C. Following slow cooling, vials were transferred directly to liquid nitrogen.…”

Section: Cryopreservation Of Proliferating Embryogenic Linesmentioning

confidence: 99%

Simple and efficient protocols for the initiation and proliferation of embryogenic tissue of Douglas-fir

Reeves

Hargreaves

Trontin

et al. 2017

Trees

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“…By contrast, von Arnold et al (1995) found this response to be a random effect and has not been evident in larger arrays of genotypes (Norgaard et al 1993b. A lag-phase is observed during regeneration in many (Galerne and Dereuddre 1987, Laine et al 1992, Norgaard et al 1993b but not all studies (Find et al 1993), and prethaw multiplication rates are established after one or more subculture intervals . The proportion and organization of embryogenic cells (Laine et al 1992, Norgaard et al 1993b, culture growth stage (Charest and Klimaszewska 1995), vial source (Hargreaves et al 1995), and nurse cultures (Hargreaves et al 1995) have all been cited as factors affecting post-thaw regeneration .…”

Section: 2 Freezing Approachesmentioning

confidence: 78%

“…Plants have been found to be phenotypically normal (Laine et a/. al 1992, Find et al 1993, Gupta et a/. 1993, Hargreaves and Smith 1994b and have been established in field trials (von Arnold et al 1995, Klimaszewska et al 1992.…”

Section: Somatic Embryo and Plant Regenerationmentioning

confidence: 98%

Cryopreservation of Embryogenic Cultures of Conifers and Its Application to Clonal Forestry

Cyr

1999

Somatic Embryogenesis in Woody Plants

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Cryopreservation of an embryogenic suspension culture of Picea sitchensis and subsequent plant regeneration

Cited by 28 publications

References 13 publications

Embryogenic tissue initiation and somatic embryogenesis in Fraser fir (Abies fraseir [Pursh] Poir.)

Embryogenic tissue initiation and somatic embryogenesis in Fraser fir (Abies fraseir [Pursh] Poir.)

Simple and efficient protocols for the initiation and proliferation of embryogenic tissue of Douglas-fir

Cryopreservation of Embryogenic Cultures of Conifers and Its Application to Clonal Forestry

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