Cryopreservation of allogeneic PBSC from related and unrelated donors is associated with delayed platelet engraftment but has no impact on survival

Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

“…The observed frequency of approved cryopreservation requests associated with activity regarding adult unrelated donors managed through the OneMatch Stem Cell and Marrow Network suggests an increased frequency compared with more historical data of less than 2% reported by Medd and colleagues from the Center for Clinical Haematology, Queen Elizabeth Hospital, Birmingham, UK . It was interesting to see that most of the cryopreserved requests implicated unrelated donors from international sources.…”

“…L 7,14,15,[17][18][19]21,[26][27][28][30][31][32][33][34][36][37][38] ) was reported in any of the studies in comparison to controls (Table 5). Moreover, no apparent difference in CD341 cells infused (eight studies 9,14,[17][18][19]21,29,31 ) was reported for patients receiving cryopreserved products compared to controls receiving fresh products ( Table 5). The yield of CD341 cells available after thawing was 31% to 100% (data reported in eight studies 9,13,21,24,26,[35][36][37] ).…”

“…The yield of CD341 cells available after thawing was 31% to 100% (data reported in eight studies 9,13,21,24,26,[35][36][37] ). Only 13 studies 12,13,[16][17][18][19][20]26,30,32,35,36,38 (40% of studies, reporting on 575 patients or 57.9% of total) reported reasons for performing cryopreservation. While most studies reported more than one reason for performing cryopreservation, few specified the precise number of products for each cited reason.…”

Engraftment for CD34 selected stem cell products is not compromised by cryopreservation

“…There are emerging data that support the idea that allogeneic stem cell products can be safely cryopreserved . However, reports with cryopreserved PBSCs have also shown increased infusion reactions, delayed PLT engraftment, and possible impact on chronic GVHD . There have also been concerns about reduced function of immune effector T cells with cryopreservation…”

“…Cryopreserved products may enhance safety by guaranteeing the availability of a quality‐controlled graft before initiating myeloablative therapy. Although there is no known correlation between the infusion of cryopreserved HPCs and a decreased overall survival and cryopreservation is generally considered to be safe, a few studies previously reported that it is associated with infusion reactions, delayed platelet (PLT) engraftment, and possibly chronic GVHD . To assess whether cryopreservation of the CD34+‐selected product compromises transplant outcomes, we performed a retrospective analysis of neutrophil and PLT engraftment for haploidentical products that were freshly infused versus cryopreserved, thawed, and infused.…”