Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberry

Niino, Toshiki; Sakai, Akira

doi:10.1016/0168-9452(92)90151-b

Cited by 131 publications

(49 citation statements)

References 13 publications

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“…Cold preculture of donor plants prior to cryopreservation is known to improve results for woody (Niino and Sakai 1992;Reed et al 2003; and herbal (Seibert and Wetherbee 1977;Keller 2005) species, which are naturally able to cold acclimate at low temperatures. Also in other species like Zoysia matrella, Zoysia japonica and yam (Dioscorea spp.…”

Section: Donor Culturesmentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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Potato is one of the most important crops worldwide. Genetic resources of potato (Solanum tuberosum L. ssp. tuberosum) and related cultivated species are conserved through storage of tubers, in vitro plants and in cryopreservation. Cryopreservation, storage in or above liquid nitrogen, is the best option to maintain vegetatively propagated plants in the long term. The present review gives comprehensive information about various cryopreservation techniques for potato published from 1977 until the present. It discusses factors that affect the process and success of cryopreservation, such as donor culture conditions, preculture, cooling, warming and post-culture treatments. Studies are presented that analyse the histological and ultrastructural changes after different cryopreservation steps and the morphological pathways during regeneration of plants after rewarming. The maintenance of genetic stability in potato after cryopreservation has also been demonstrated by various phenotypic and molecular methods. The first thermal analyses on potato shoot tips are presented using differential scanning calorimetry to analyse the state of water during cooling and warming. Biochemical analyses of different compounds, such as soluble sugars and proteins, have been performed to understand and improve existing cryogenic methods. Potato is an example where successful virus elimination has been obtained via cryopreservation of shoot tips (cryotherapy). There are already cryopreserved collections of potato shoot tips in

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Section: Donor Culturesmentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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“…75 Men preculture directe et jusqu' a 1 Men preculture progressive). Des concentrations similaires se sont revelees efficaces pour laconservation a -196°C d'apex d'autres especes, en preculture progressive telles que la vigne (Plessis et a!., 1991(Plessis et a!., , 1993, 1' eucalyptus (Poissonier et al, 1991 ), le pommier et le poirier (Niino et Sakai, 1992) et la chicoree (Vandenbussche et al, 1993). Chez la pomme de terre, les clones diploldes ont mieux resiste a la deshydratation eta la congelation que les clones tetraploi"des notamment le SI22 avec des taux de survie de 75 % et 64% respectivement apres deshydratation et congelation, lorsque le milieu de preculture est enrichi progressivement en sucre, de 0.3 a 1M et en absence d'hormones.…”

Section: Discussionunclassified

Effets d'une préculture sur la tolérance à la congélation dans I'azote liquide des apex axillaires de vitroplants de Solanacées tubérifères (Solanum phurejaetS. tuberosum). Etude après enrobage et déshydratation

Bouafia¹,

Lairy²,

Blanc³

et al. 1995

Acta Botanica Gallica

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“…Alginate gels appear to be adequate for cryopreservation of in vitro-grown shoot tips of different plant species (Niino & Sakai, 1992). Cryopreservation of fungal spores using porous beads was also achieved by Chandler (1994), and ectomycorrhizal fungi entrapped in alginate gels have been shown to retain their infectivity after storage at 4mC for several months (Mauperin et al, 1987).…”

Section: mentioning

confidence: 99%

Cryopreservation of entrapped monoxenically produced spores of an arbuscular mycorrhizal fungus

Declerck

Coppenolle

2000

New Phytologist

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A study was conducted to quantify the ability of entrapped, monoxenically produced spores of an arbuscular mycorrhizal fungus to germinate and reproduce the fungal life cycle after cryopreservation. No germination was obtained after incubation of entrapped spores in glycerol and mannitol and subsequent cryopreservation at k70mC, regardless of the concentration of cryoprotectants and duration of incubation. Incubation for 1 d in 0n5 M sucrose, and for 1 and 2 d in 0n5 M trehalose, led to spore germination after cryopreservation at k70mC. Lower cryopreservation temperatures were tested with entrapped spores incubated for 1 d in 0n5 M trehalose. The highest germination rate, estimated by the percentage of potentially infective beads (%PIB), was obtained at k100mC. A %PIB of 95% (water agar medium) to 100% (Strullu-Romand medium) was obtained at this temperature. Thereafter, %PIB rapidly decreased at k140 and k180mC. Heavy sporulation and high internal root colonization were obtained after re-association of the entrapped spores, incubated for 1 d in 0n5 M trehalose and subsequently cryopreserved at k100mC, with transformed carrot roots. This demonstrates the ability of entrapped spores to reproduce the fungal life cycle following cold treatment.

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Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberry

Cited by 131 publications

References 13 publications

Potato Shoot Tip Cryopreservation. A Review

Potato Shoot Tip Cryopreservation. A Review

Effets d'une préculture sur la tolérance à la congélation dans I'azote liquide des apex axillaires de vitroplants de Solanacées tubérifères (Solanum phurejaetS. tuberosum). Etude après enrobage et déshydratation

Cryopreservation of entrapped monoxenically produced spores of an arbuscular mycorrhizal fungus

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