Cryopreservation of adipose tissues: The role of trehalose

Pu, Lee Li-Qun; Cui, Xianwei; Fink, Betsy F.; Cibull, Michael L.; Gao, Dayong

doi:10.1016/j.asj.2005.01.003

Cited by 33 publications

(28 citation statements)

References 24 publications

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“…Therefore, the concentration of FBS in the cryomedium is reduced to 20% (a general concentration of FBS used for cryopreservation of hMSCs) (Liu et al ., , ; Zhang et al ., ) or 0% (xeno‐free cryomedium). Meanwhile, trehalose, a non‐permeating or extracellular CPA, at a concentration of 0.25 m , was effective in preserving viability and functional properties of adipocytes from human adipose tissues (Pu et al ., ), suggesting its potential as an alternative to DMSO and FBS to preserve hASCs isolated from human adipose tissues.…”

Section: Resultsmentioning

confidence: 99%

Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells

Yong

Safwani

et al. 2016

J Tissue Eng Regen Med

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Cryopreservation represents an efficient way to preserve human mesenchymal stem cells (hMSCs) at early culture/passage, and allows pooling of cells to achieve sufficient cells required for off-the-shelf use in clinical applications, e.g. cell-based therapies and regenerative medicine. To fully apply cryopreserved hMSCs in a clinical setting, it is necessary to evaluate their biosafety, e.g. chromosomal abnormality and tumourigenic potential. To date, many studies have demonstrated that cryopreserved hMSCs display no chromosomal abnormalities. However, the tumourigenic potential of cryopreserved hMSCs has not yet been evaluated. In the present study, we cryopreserved human adipose-derived mesenchymal stem cells (hASCs) for 3 months, using a slow freezing method with various cryoprotective agents (CPAs), followed by assessment of the tumourigenic potential of the cryopreserved hASCs after thawing and subculture. We found that long-term cryopreserved hASCs maintained normal levels of the tumour suppressor markers p53, p21, p16 and pRb, hTERT, telomerase activity and telomere length. Further, we did not observe significant DNA damage or signs of p53 mutation in cryopreserved hASCs. Our findings suggest that long-term cryopreserved hASCs are at low risk of tumourigenesis. These findings aid in establishing the biosafety profile of cryopreserved hASCs, and thus establishing low hazardous risk perception with the use of long-term cryopreserved hASCs for future clinical applications. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells

Yong

Safwani

et al. 2016

J Tissue Eng Regen Med

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“…2,25,26 Trehalose, a nonreducing disaccharide of glucose, has been proposed to be an exceptional cryoprotectant with excellent biocompatibility for cell cryopreservation. 27−32 Because of its inability to penetrate the cell plasma membrane, current research with trehalose for cell cryopreservation has been focused on either combining it with pCPAs to reduce the pCPA concentration or developing methods to deliver trehalose into cells. 20,33−35 However, the former could not eliminate the pCPAs and the latter is still elusive today.…”

Section: Introductionmentioning

confidence: 99%

Predehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation

Huang

Zhao

Zhang

et al. 2017

ACS Biomater. Sci. Eng.

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Conventional approaches for cell cryopreservation require the use of toxic membrane-penetrating cryoprotective agents (pCPA), which limits the clinical application of cryopreserved cells. Here, we show intentionally induced ice formation at a high subzero temperature (> −10 °C) during cryopreservation, which is often referred to as ice seeding, could result in significant cell injury in the absence of any pCPA. This issue can be mitigated by predehydrating cells using extracellular trehalose to their minimal volume with minimized osmotically active water before ice seeding. We further observe that ice seeding can minimize the interfacial free energy that drives the devastating ice recrystallization-induced cell injury during warming cryopreserved samples. Indeed, by combining predehydration using extracellular trehalose with ice seeding at high subzero temperatures, high cell viability or recovery is achieved for fibroblasts, adult stem cells, and red blood cells after cryopreservation without using any pCPA. The pCPA-free technology developed in this study may greatly facilitate the long-term storage and ready availability of living cells, tissues, and organs that are of high demand by modern cell-based medicine.

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“…79 In fact, cryopreservation of fat tissue is not a new research area and has been extensively investigated in large scale adipose tissue engineering, especially in applications of maxillofacial and craniofacial surgery. Although many studies found that freezing fat grafts by using optimal cryopreservation techniques enables their longterm preservation, [80][81][82][83][84][85][86] only few considered whether the frozen fat tissue could still be a reliable source of adult stem cells. 87,88 Further studies are required to determine whether cryopreserved fat tissue still maintains the viable and potent adult stem cells in quantities required for clinical needs.…”

Section: Clinical Banking Of Adipose Derived Adult Stem Cells (Ascs)mentioning

confidence: 99%

Clinical grade adult stem cell banking

2009

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Cryopreservation of adipose tissues: The role of trehalose

Cited by 33 publications

References 24 publications

Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells

Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells

Predehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation

Clinical grade adult stem cell banking

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