Cryopreservation media differentially affect sperm motility, morphology and DNA integrity

Raad, Georges; Lteif, L.; Lahoud, R.; Tanios, Judy; Hazzouri, Mira; Azoury, J.

doi:10.1111/andr.12531

Cited by 43 publications

(58 citation statements)

References 57 publications

(90 reference statements)

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“…As expected, post‐thawed sperm parameters were significantly decreased compared to the pre‐freeze state. Our results confirmed Raad et al () findings who demonstrated that frozen‐thawed spermatozoa exhibited a decline in sperm quality in normozoospermic samples compared to fresh semen (Raad et al, ). Similarly, Zribi et al () reporting on sperm motility, viability and DNA integrity before and after cryopreservation with supplementation of antioxidants in the freezing medium, showed that the motility and viability of spermatozoa were reduced after the freezing–thawing process.…”

Section: Discussionsupporting

confidence: 92%

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

et al. 2020

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The objective was to evaluate the effect of inclusion of 2.5% and 5% ovine serum, enriched with vitamin E (Vit E) and fish oil (FO), in human sperm freezing medium. Serum samples were prepared from sixteen rams (n = 4) feeding on a without supplemented diet, and diets supplemented with Vit E, FO and Vit E + FO. Semen samples, from 60 normozoospermic men, were frozen in: (I) a commercial freezing medium (SpermFreeze™; control medium), (II) the commercial freezing medium containing foetal bovine serum, (III) the commercial freezing medium + nonenriched serum (serum group), (IV) the commercial freezing medium + Vit E enriched serum (Vit E group), (V) the commercial freezing medium + FO enriched serum (FO group) and (VI) the commercial freezing medium + Vit E + FO enriched serum (Vit E + FO group). Sperm total and progressive motility, morphology, viability and plasma membrane integrity were significantly higher (p ≤ .05) in Vit E and Vit E + FO groups compared with the control group. Mitochondrial membrane potential did not differ between treatments (p > .05). It was concluded that ovine serum enriched with vitamin E and vitamin E + FO improved the quality of human spermatozoa but enriched serum containing FO could not improve the sperm cryo‐injuries.

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Section: Discussionsupporting

confidence: 92%

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

et al. 2020

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“…Although sperm cells have the ability to adapt to osmotic changes [ 79 ], freezing medium does not fully protect them from the osmotic stress happening during freezing and thawing that leads to an increase in ROS production, apoptosis-like changes, DNA damage, and an increase in tail defects [ 80 , 81 , 82 ]. Therefore, we hypothesized that the addition of cAMSC-CM to the freezing medium would increase sperm tolerance to osmotic changes, oxidative defence, and help protect their ultra-structure, because it contains proteins with anti-oxidant, regenerative, and anti-apoptotic effects ( Table S2 ).…”

Section: Discussionmentioning

confidence: 99%

“…The cryopreservation process leads to an increase in sperm tail abnormalities [ 80 , 84 ], altered acrosome and plasma membrane integrity [ 85 ], and a decrease in the mitochondrial activity [ 4 , 82 ] and motility [ 80 , 82 ]. In our study, we found that the percentage of coiled tail sperm was the same in both groups, but the percentage of sperm with a bent tail was significantly reduced in the 10% CM-treated group in comparison with the control group ( Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Mahiddine

Kim

Qamar

et al. 2020

Animals

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This study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared from cAMSCs was subjected to proteomic analysis for the identification of proteins present in the medium. Sperm samples were treated with freezing medium supplemented with 0%, 5%, 10%, and 15% of the CM, and kinetic parameters were evaluated after 4–6 h of chilling at 4 °C to select the best concentration before proceeding to cryopreservation. Quality-related parameters of frozen–thawed sperm were investigated, including motility; kinetic parameters; viability; integrity of the plasma membrane, chromatin, and acrosome; and mitochondrial activity. The results showed that 10% of the CM significantly enhanced motility, viability, mitochondrial activity, and membrane integrity (p < 0.05); however, the analysis of chromatin and acrosome integrity showed no significant differences between the treatment and control groups. Therefore, we concluded that the addition of 10% CM derived from cAMSC in the freezing medium protected dog sperm during the cryopreservation process.

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“…Laboratory conditions (i.e., prolonged incubation, centrifugation, cryopreservation and use of different media) can significantly impact SDF by increasing OSmediated DNA damage [228][229][230][231]. Conventional (swimup, DGC) and advanced techniques can select sperm with low levels of SDF [232][233][234][235][236] (Table 3).…”

Section: Sperm Processing and Preparationmentioning

confidence: 99%

Sperm DNA Fragmentation: A New Guideline for Clinicians

Agarwal

Majzoub

Baskaran

et al. 2020

World J Mens Health

169

144

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Sperm DNA integrity is crucial for fertilization and development of healthy offspring. The spermatozoon undergoes extensive molecular remodeling of its nucleus during later phases of spermatogenesis, which imparts compaction and protects the genetic content. Testicular (defective maturation and abortive apoptosis) and post-testicular (oxidative stress) mechanisms are implicated in the etiology of sperm DNA fragmentation (SDF), which affects both natural and assisted reproduction. Several clinical and environmental factors are known to negatively impact sperm DNA integrity. An increasing number of reports emphasizes the direct relationship between sperm DNA damage and male infertility. Currently, several assays are available to assess sperm DNA damage, however, routine assessment of SDF in clinical practice is not recommended by professional organizations. This article provides an overview of SDF types, origin and comparative analysis of various SDF assays while primarily focusing on the clinical indications of SDF testing. Importantly, we report four clinical cases where SDF testing had played a significant role in improving fertility outcome. In light of these clinical case reports and recent scientific evidence, this review provides expert recommendations on SDF testing and examines the advantages and drawbacks of the clinical utility of SDF testing using Strength-Weaknesses-Opportunities-Threats (SWOT) analysis.

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Cryopreservation media differentially affect sperm motility, morphology and DNA integrity

Abstract: This study indicates that the recovery rate of competent spermatozoa, after cryopreservation, is still critical in infertile men. Therefore, frozen semen sample should be used only when necessary.

Cited by 43 publications

References 57 publications

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters

Sperm DNA Fragmentation: A New Guideline for Clinicians

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