Cryopreservation induces temporal DNA methylation epigenetic changes and differential transcriptional activity in Ribes germplasm

Johnston, Jason W.; Benson, Erica E.; Harding, Keith

doi:10.1016/j.plaphy.2008.10.008

Cited by 65 publications

(39 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with differences in global DNA methylation between meristematic areas of juvenile and mature Pinus radiata (Fraga et al 2002). In contrast, no changes in DNA methylation levels were observed in regenerated shoots of Ribes during 140 days of in vitro culture (Johnston et al 2009). These data indicate that global DNA methylation in the seedlings that developed from EAs after cryopreservation was not static and that it had unique dynamics during each developmental stage.…”

Section: Dna Methylation During In Vitro Culturesupporting

confidence: 82%

“…These findings suggest that molecular changes may be essential in modulating the tolerance of F. sylvatica tissues to cold stress. Similarly, the levels of DNA methylation were decreased in the meristems of currants (Ribes) with susceptible genotypes; in tolerant genotypes, the number of methylated DNA sequences increased compared to controls (Johnston et al 2009). However, research by Peredo et al (2009) did not show epigenetic changes in two hop accessions (Humulus lupulus) after 2 years of in vitro micropropagation and after 12 cycles compared to field plants.…”

Section: Dna Methylation During In Vitro Culturementioning

confidence: 96%

“…However, analysis of DNA methylation post-cryo culture can be an indicator of plant adaptation to low temperature stress, as well as regeneration of plant material (Johnston et al 2009). Evidence suggests that after undergoing stresses, plants may change their response to a future stress (Zeng et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The aim of effective cryopreservation is the long-term preservation of plant material, and it is important to know whether this induces permanent genetic changes. Changes in the level of DNA methylation were observed in papaya (Kaity et al 2008), chrysanthemum (Martín and González-Benito 2006;Martin et al 2011) currants (Johnston et al 2009), strawberries (Hao et al 2002a), citrus (Hao et al 2002b) and potatoes (Kaczmarczyk et al 2012). Changes in global DNA methylation may also be induced by cryovitrification agents and the conditions of in vitro culturing (Harding 2004).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Nuc

Marszałek

Pukacki

2016

Trees

View full text Add to dashboard Cite

Key message Dehydration and cryo-stress (2196°C) caused a greater increase in the DNA methylation of embryonic axes (EAs) of beech seeds (tolerant to both stresses) than in EAs of oak (sensitive to both stresses). Simultaneously, in vitro survival of cryopreserved beech EAs was also significantly higher than in those of oak. Abstract DNA methylation plays a key role in the regulation of growth and differentiation in plants. The present study focused on global DNA methylation of embryonic axes (EAs) isolated from the seeds of pedunculate oak (Quercus robur L.) and European beech (Fagus sylvatica L.) after cryopreservation. Oak seeds can be labeled recalcitrant, i.e., sensitive to dehydration, while beech seeds fall under the suborthodox category, i.e., tolerant to dehydration. DNA methylation was determined using a specific antibody directed at the methylated cytosine in CpG dinucleotides. The analysis was carried out on DNA isolated from EA tissues and subjected to (1) dehydration (control), (2) a combination of dehydration and plant vitrification solution (PVS3) and (3) dehydration with vitrification PVS3, and rapid cooling in liquid nitrogen (LN 2 , -196°C) at a cooling rate of 32°C s -1 . Such treatments induced global DNA methylation primarily in tolerant EAs from F. sylvatica seeds. The level of global DNA methylation in this material was 30 % higher than in the control, i.e., dehydrated (not frozen) beech EAs. The same treatment in oak EAs did not evoke such changes. After 120 days of tissue growth, we observed substantially more DNA demethylation in the beech tissues after cryopreservation in LN 2 than in the controls or in tissues subjected to vitrification but not cryopreservation. Information on the global level of DNA methylation can be useful in monitoring procedures aimed at increasing the rate of recovery of EAs following cryopreservation.

show abstract

Section: Dna Methylation During In Vitro Culturesupporting

confidence: 82%

Section: Dna Methylation During In Vitro Culturementioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Nuc

Marszałek

Pukacki

2016

Trees

View full text Add to dashboard Cite

show abstract

“…Ribosomal fragment variability possibly results from restriction enzyme sensitivity to methylation of enzyme recognition sites (Harding 1997). DNA methylation as an epigenetic mechanism for genomic variability (Harding 2004, Harding et al 2008) may also account for physiological variation between individual meristems and their differential responses (Johnston et al 2007, Johnston et al 2009). Although, in vitro variation within batches of individual meristems clearly exists, it is not inherently obvious prior to cryopreservation and this makes its detection difficult.…”

Section: Cryoinjury Genetic Stability and Cryobionomicsmentioning

confidence: 99%

Exploring the physiological basis of cryopreservation success and failure in clonally propagated in vitro crop plant germplasm

Harding¹,

Johnston²,

Benson³

2008

AFSci

View full text Add to dashboard Cite

An appraisal of potato and Ribes shoot meristem cryopreservation shows physiological factors influence survival and development, sometimes independently of protocol and genotype. Markers for oxidative damage incurred by cryostorage reveal two responses: (1) oxidative stress with an eventual decline in regrowth and (2) an oxidative burst associated with higher survival. Differential responses to cryoinjury are discussed in relation to in vitro ageing and genetic stability within the conceptual framework of cryobionomics. The possibility that cryopreservation-induced cell death and apoptosis occurs in plants is considered with respect to current concepts of animal cell cryoinjury. It is proposed that a more holistic approach is now required to understand the basis for success or failure of cryopreserved plant germplasm.

show abstract

Cryopreservation of Plant Cells

Keller¹,

Senula²,

Höfer³

et al. 2013

Encyclopedia of Industrial Biotechnology

View full text Add to dashboard Cite

Cryopreservation, storage of living tissues in or above liquid nitrogen, is increasingly used to preserve germplasm in the long term. When cooling plant tissue to low temperatures, water may destroy cells by osmotic drying or disruption of organelles by forming ice crystals. The organisms accumulate cryoprotective substances to adapt to freezing. Cryoprotectants trigger glass transition (vitrification), when temperature is cooled down rapidly. In the course of cryopreservation, tissues are imposed by various influences causing oxidative stress: wounding, dehydration, and direct liquid nitrogen effect. Occurring reactive oxygen species may be measured and antioxidant substances may be added. Cell structure alterations, water status, regeneration from cryopreservation injury, and genetic and epigenetic changes, which may occur, were investigated. Various plant tissues were cryopreserved: dedifferentiated cells and tissues; shoot tips; axillary buds and other meristematic plant parts; dormant buds of woods species, seeds, embryos, and embryo axes; and pollen. Several main methods were used: slow freezing, vitrification, and encapsulation. Liquid nitrogen can also be used to eliminate viruses from the organs (cryotherapy). The existence of endogenous or contaminating microorganisms needs special attention. Furthermore, technological considerations and the use of cryopreservation to maintain transgenic or patented material need to be mentioned.

show abstract

Cryopreservation induces temporal DNA methylation epigenetic changes and differential transcriptional activity in Ribes germplasm

Cited by 65 publications

References 30 publications

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Cryopreservation changes the DNA methylation of embryonic axes of Quercus robur and Fagus sylvatica seeds during in vitro culture

Exploring the physiological basis of cryopreservation success and failure in clonally propagated in vitro crop plant germplasm

Cryopreservation of Plant Cells

Contact Info

Product

Resources

About