Cryopreservation Impacts Cell Functionality of Long Term Expanded Adipose-Derived Stem Cells

Othmani, Ali El; Rouam, Sabrina; Abbad, Anass; Erraoui, Chaimaa; Harriba, Sara; Boukind, Hassan; Nourlil, Jalal; Malka, G.; Mazini, Loubna

doi:10.4172/2157-7633.1000445

Cited by 13 publications

(10 citation statements)

References 49 publications

(66 reference statements)

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“…These growth factors were reported to autoactivate ADSCs and initiate the whole mechanisms involved in different phases of wound healing [52]. Indeed, ADSCs secretome is composed of proinflammatory and anti-inflammatory growth factors, fibroblasts growth factors including GDF11, TGF-β, b-FGF, VEGF, TLR2, TLR4, IL-10, and MMP involved directly in cell proliferation, and tissue repair and rejuvenation processes [61,111,[149][150][151][152]. These factors are consistently responsible for the inflammatory status of the immune skin cells and their impact on the nature and efficacy of healing is more appropriate by modulating the skin microenvironment, also suggesting the implication of ADSCs.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Mazini

Rochette

Admou

et al. 2020

IJMS

Self Cite

316

219

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Adipose tissue derived stem cells (ADSCs) are mesenchymal stem cells identified within subcutaneous tissue at the base of the hair follicle (dermal papilla cells), in the dermal sheets (dermal sheet cells), in interfollicular dermis, and in the hypodermis tissue. These cells are expected to play a major role in regulating skin regeneration and aging-associated morphologic disgraces and structural deficits. ADSCs are known to proliferate and differentiate into skin cells to repair damaged or dead cells, but also act by an autocrine and paracrine pathway to activate cell regeneration and the healing process. During wound healing, ADSCs have a great ability in migration to be recruited rapidly into wounded sites added to their differentiation towards dermal fibroblasts (DF), endothelial cells, and keratinocytes. Additionally, ADSCs and DFs are the major sources of the extracellular matrix (ECM) proteins involved in maintaining skin structure and function. Their interactions with skin cells are involved in regulating skin homeostasis and during healing. The evidence suggests that their secretomes ensure: (i) The change in macrophages inflammatory phenotype implicated in the inflammatory phase, (ii) the formation of new blood vessels, thus promoting angiogenesis by increasing endothelial cell differentiation and cell migration, and (iii) the formation of granulation tissues, skin cells, and ECM production, whereby proliferation and remodeling phases occur. These characteristics would be beneficial to therapeutic strategies in wound healing and skin aging and have driven more insights in many clinical investigations. Additionally, it was recently presented as the tool key in the new free-cell therapy in regenerative medicine. Nevertheless, ADSCs fulfill the general accepted criteria for cell-based therapies, but still need further investigations into their efficiency, taking into consideration the host-environment and patient-associated factors.

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Section: Summary and Perspectivesmentioning

confidence: 99%

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Mazini

Rochette

Admou

et al. 2020

IJMS

Self Cite

316

219

View full text Add to dashboard Cite

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“…Even ADSCs were more susceptible to differentiate into the adipogenic lineage when compared to BM- and UC-MSCs, their multipotency was appreciated for ectodermic and endodermic tissue repair [6,30,47,81] (Figure 2). Nevertheless, their functionality was impacted by long term expansion culture and cryopreservation [32,64,82], which suggests that further studies should be performed to standardize ADSCs manipulation for clinical use.…”

Section: Types Of Stem Cellsmentioning

confidence: 99%

“…Even AT has important implications in the development of stem cell bank [242,243,244], the interest raises by ADSCs have improved two specific questions: first, their use must be practical and effective (high numbers of cells are needed); and second, the clinical outcomes should be identical to the expected use. A major advantage is that of ADSCs could be maintained for up to 24–48 h within lipoaspirates [82], cryopreserved before separation and seeding in culture with a stable and efficient ability to proliferate and differentiate [245,246], while fresh BM and UC tissues are necessary for the collection and deriving of MSCs. Consequently, the banking of allogeneic ADSCs remains full of hope for the future regenerative medicine.…”

Section: Issues Related To Autologous and Allogeneic Clinical Use mentioning

confidence: 99%

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs)

Mazini

Rochette

Amine

et al. 2019

IJMS

Self Cite

297

212

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Adipose tissue is now on the top one of stem cell sources regarding its accessibility, abundance, and less painful collection procedure when compared to other sources. The adipose derived stem cells (ADSCs) that it contains can be maintained and expanded in culture for long periods of time without losing their differentiation capacity, leading to large cell quantities being increasingly used in cell therapy purposes. Many reports showed that ADSCs-based cell therapy products demonstrated optimal efficacy and efficiency in some clinical indications for both autologous and allogeneic purposes, hence becoming considered as potential tools for replacing, repairing, and regenerating dead or damaged cells. In this review, we analyzed the therapeutic advancement of ADSCs in comparison to bone marrow (BM) and umbilical cord (UC)-mesenchymal stem cells (MSCs) and designed the specific requirements to their best clinical practices and safety. Our analysis was focused on the ADSCs, rather than the whole stromal vascular fraction (SVF) cell populations, to facilitate characterization that is related to their source of origins. Clinical outcomes improvement suggested that these cells hold great promise in stem cell-based therapies in neurodegenerative, cardiovascular, and auto-immunes diseases.

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“…Senescent ADSCs were likely found to be impacted in their ability to sustain tissue hemostasis and hence resulted in loss of tissue and organ integrity. Even these cells display a rich secretory profile; their ability to secrete ECM proteins, cytokines, and chemokines was largely impaired in vitro during culture expansion [107].…”

Section: Cellular Implicationmentioning

confidence: 99%

Adipose-Derived Stem Cells (ADSCs) and Growth Differentiation Factor 11 (GDF11): Regenerative and Antiaging Capacity for the Skin

Mazini¹,

Rochette²,

Malka³

2020

Regenerative Medicine

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Adipose-derived stem cells (ADSCs) have proven their efficiency in wound healing and skin regeneration in vitro and in vivo. They were reported to differentiate into skin cell types and migrate to wounded sites to restore cell deficiencies and functions. Secretome of ADSCs is involved in the migration and proliferation of dermal fibroblasts (DFs) and keratinocytes where growth differentiation factor 11 (GDF11) and transforming growth factor-β (TGF-β) are expected to play the principal role. Both factors are implicated in immune responses, skin cell differentiation, proliferation and pigmentation, migration and secretion of the extracellular matrix proteins. Increasing evidence has pointed the fact that ADSCs are expected to cross-react with GDF11 to ensure DF and keratinocytes proliferation to reverse the aging process. Moreover, these factors share similar intracellular mechanisms pathways that are SMAD-dependent, and target different cellular mechanisms related to regeneration or rejuvenation. This intriguing balance between GDF11dependent aging and TGF-β-dependent regeneration still remains unclear and might be regulated in a spatio-temporal manner. Considering the clinical relevance of the mechanisms slowing or delaying the onset of age, we aimed to clarify the involvement of cell signaling pathways related to GDF11 and TGF-β in balancing cell rejuvenation and cell regeneration. Increasing the organ lifespan and functionality might be challenging issues.

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Cryopreservation Impacts Cell Functionality of Long Term Expanded Adipose-Derived Stem Cells

Cited by 13 publications

References 49 publications

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Regenerative Capacity of Adipose Derived Stem Cells (ADSCs), Comparison with Mesenchymal Stem Cells (MSCs)

Adipose-Derived Stem Cells (ADSCs) and Growth Differentiation Factor 11 (GDF11): Regenerative and Antiaging Capacity for the Skin

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