Cryopreservation as a Tool for the Management of Coconut Germplasm

Malaurie, Bernard; Tregear, James; N'Nan, Oulo; Bandupriya, H.D.D.; Borges, M.; Verdeil, Jean Luc

doi:10.17660/actahortic.2011.908.59

Cited by 3 publications

(2 citation statements)

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“…In their study on coconut, Malaurie et al indicated that explants, such as plumules, with a small size (ca. 1 mm) lead to a higher density of meristematic cells in coconut tissue culture [40]. Bazrafshan (2022) justified that the size of the embryo may have notable impacts on the embryos' germination ability, as well as on subsequent steps, such as tissue culture and cryopreservation [19].…”

Section: Discussionmentioning

confidence: 99%

The Influence of Maturity, Storage, and Embryo Size on Coconut Callus Induction Success

Mu,

Yang,

et al. 2024

Forests

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Coconut palms (Cocos nucifera L.) are globally significant palms with both economic and cultural value. Despite the increasing demand for coconut products, production is decreasing globally due to palm senility, pests, and diseases. It has been estimated that over half of the world’s coconut palms need to be replaced immediately. The coconut industry has acknowledged that conventional propagation methods are unlikely to yield sufficient high-quality planting material. Therefore, coconut tissue culture is considered a potential solution to this problem. By using coconut tissue culture, a large number of plantlets can be obtained in a short period of time. In this study, the quality of explants and the development stage (visible shoot/non-visible shoot) of coconut used for micropropagation were examined. To our knowledge, little research has been undertaken on this aspect of coconut micropropagation. Our results indicated that tender coconut fruit exhibited an advantage over mature fruits. In addition, coconut plumule explants subjected to an extended storage of 15 days demonstrated enhanced development compared to those without storage. Notably, smaller embryos utilized as explants displayed superior callus formation compared to their larger counterparts. Finally, embryos possessing shoots exhibited improved callus initiation, albeit accompanied by a more pronounced browning effect. Further investigations are required to obtain more knowledge about the most suitable conditions for plumule explants that lead to optimal callus initiation.

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Section: Discussionmentioning

confidence: 99%

The Influence of Maturity, Storage, and Embryo Size on Coconut Callus Induction Success

Mu,

Yang,

et al. 2024

Forests

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“…bras., Brasília, v.47, n.1, p.8-13, jan. 2012 'Rennel Tall' (RT). Later, other researchers, such as Karun et al (2005Karun et al ( , 2006, Malaurie et al (2006Malaurie et al ( , 2011, Sajini et al (2006), Bandupriya et al (2007), N 'Nan et al (2008), Sisunandar et al (2010), among others, published promising results with cryopreservation using zygotic embryos, plumules and pollen as explants.…”

Section: Introductionmentioning

confidence: 99%

Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching

Tavares

Almeida

Pereira

2012

Pesq. agropec. bras.

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The objective of this work was to adapt the application of electrolytic conductivity and potassium leaching tests to assess the viability of cryopreserved embryos of 'Anão Verde do Brasil de Jiqui' (AVeJBr) coconut. The zygotic embryos were excised, sterilized and subjected to four cryoprotectant treatments combined with three incubation times (12, 16 and 20 hours), totaling 12 treatments. The pre-treatment of mature zygotic embryos of AVeJBr coconut using a cryoprotectant with 1.75 mol L-1 of sucrose + 15% glycerol for 12 and 16 hours promoted lower embryo humidity and increased viability in electrolytic conductivity and potassium leaching tests. Samples with ten embryos are sufficient for electrolytic conductivity analysis in cryopreserved or non-cryopreserved AVeJBr coconut zygotic embryos. The 4 to 8 hour imbibition period of the embryos is promising for the electrolytic conductivity analysis of non-cryopreserved mature zygotic embryos of AVeJBr coconut.

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A practical framework for the cryopreservation of palm species

Nguyen

et al. 2023

In Vitro Cell.Dev.Biol.-Plant

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Palms (Arecaceae) contribute remarkable economic and environmental values to human life. However, many elite and commercial palm species are becoming critically endangered and demand immediate action to preserve their genetic resources. Cryopreservation has established itself as the definitive in vitro conservation method for recalcitrant-seeded species, such as those within the Arecaceae. Progress in this area has moved steadily forward over the last three decades with the development of various techniques for different explants and palm genotypes and for molecular testing methods to ensure genetic fidelity is maintained in the regenerants. There remains a key challenge to properly identify the components that will enable the long-term conservation of palms using cryopreservation. This review methodically analyzes the state-of-the-art cryopreservation techniques developed for palms and places them within a practical framework. This framework encompasses four underlying components, namely the tissue culture approaches required, the recalcitrant nature of the palm seed, the cryobiology and cryogenic techniques required, and fidelity assessment after cryopreservation. Through a critical analysis of this framework, further optimization of palm cryopreservation protocols and more fundamental studies on the physiological and molecular changes in cryopreserved palm tissues are recommended. The present review helps to showcase a multi-decade global attempt to preserve these mostly recalcitrant species through ex situ collections. From a conservationist’s perspective, this review hopes to stimulate awareness for further concerted efforts in the conservation of rare and endangered plant families. Meanwhile, from a managerial perspective, this work serves to inform decision-makers of the global research effort underway to improve key components of the cryopreservation program for palm species and to encourage funding bodies to appropriately allocate resources to these much-needed research areas.

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Cryopreservation as a Tool for the Management of Coconut Germplasm

Cited by 3 publications

References 0 publications

The Influence of Maturity, Storage, and Embryo Size on Coconut Callus Induction Success

The Influence of Maturity, Storage, and Embryo Size on Coconut Callus Induction Success

Assessing the viability of cryopreserved coconut zygotic embryos by electrolytic conductivity and potassium leaching

A practical framework for the cryopreservation of palm species

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