Cryopreservation and validation of differentiated PDGFRα-positive cells for long term usage in experimentation
Ankita Srivastava,
Reeja Rajan,
Sallam Hasan Abdallah
et al.
Abstract:Objective
Differentiation of immortalized Mesenchymal Stromal Cells (iMSCs) into PDGFRα-positive cells under controlled growth conditions has several vital implications in functional studies concerned with the pathogenesis of Diabetic Gastroparesis (DGP). A study published previously by our research group demonstrated the importance of these cells as a novel, in-vitro model for investigating the functional role of neuronal nitric oxide synthase. The currently available methods require fresh dif… Show more
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