Cryopreservation and Proteomic Analysis of Vanilla (V. Planifolia A.) Apices Treated With Osmoprotectants

Gonzalez-Arnao, Maria Teresa; Duran-Sanchez, B.; Jiménez-Francisco, Betzaida; Lazaro-Vallejo, C. E.; Valdés‐Rodríguez, Silvia; Guerrero, Amaralina Celoto

doi:10.17660/actahortic.2011.908.5

Cited by 6 publications

(1 citation statement)

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“…Extensive stress leads to the boost of the reactive oxygen species (ROS) and the rapid development of oxidative stress (discussed below). Several studies revealed significant changes in plant tissue transcriptomic and proteomic profiles associated with various cryopreservation steps, including recovery [ 43 , 44 , 45 , 46 ]. In Arabidopsis thaliana shoot tips, the authors identified 180 transcripts that changed expression in response to cryoprotection and LN treatments, of which 67 were related to stress, defense, wounding, lipid, carbohydrate, abscisic acid, oxidation, temperature (cold/heat), or osmoregulation [ 45 ].…”

Section: Main Injuries In Plant Tissues During Cryopreservation and F...mentioning

confidence: 99%

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Попова

Куличенко

Kim

2023

Biology

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Cryopreservation is an effective option for the long-term conservation of plant genetic resources, including vegetatively propagated crops and ornamental plants, elite tree genotypes, threatened plant species with non-orthodox seeds or limited seed availability, as well as cell and root cultures useful for biotechnology. With increasing success, an arsenal of cryopreservation methods has been developed and applied to many species and material types. However, severe damage to plant material accumulating during the multi-step cryopreservation procedure often causes reduced survival and low regrowth, even when the optimized protocol is applied. The conditions at the recovery stage play a vital role in supporting material regrowth after cryopreservation and, when optimized, may shift the life-and-death balance toward a positive outcome. In this contribution, we provide an overview of the five main strategies available at the recovery stage to improve post-cryopreservation survival of in vitro plant materials and their further proliferation and development. In particular, we discuss the modification of the recovery medium composition (iron- and ammonium-free), exogenous additives to cope with oxidative stress and absorb toxic chemicals, and the modulation of medium osmotic potential. Special attention is paid to plant growth regulators used at various steps of the recovery process to induce the desired morphological response in cryopreserved tissues. Given studies on electron transport and energy provision in rewarmed materials, we discuss the effects of light-and-dark conditions and light quality. We hope that this summary provides a helpful guideline and a set of references for choosing the recovery conditions for plant species that have not been cryopreserved. We also propose that step-wise recovery may be most effective for materials sensitive to cryopreservation-induced osmotic and chemical stresses.

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Section: Main Injuries In Plant Tissues During Cryopreservation and F...mentioning

confidence: 99%

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Попова

Куличенко

Kim

2023

Biology

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Cryopreservation of Plant Cells

Keller¹,

Senula²,

Höfer³

et al. 2013

Encyclopedia of Industrial Biotechnology

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Cryopreservation, storage of living tissues in or above liquid nitrogen, is increasingly used to preserve germplasm in the long term. When cooling plant tissue to low temperatures, water may destroy cells by osmotic drying or disruption of organelles by forming ice crystals. The organisms accumulate cryoprotective substances to adapt to freezing. Cryoprotectants trigger glass transition (vitrification), when temperature is cooled down rapidly. In the course of cryopreservation, tissues are imposed by various influences causing oxidative stress: wounding, dehydration, and direct liquid nitrogen effect. Occurring reactive oxygen species may be measured and antioxidant substances may be added. Cell structure alterations, water status, regeneration from cryopreservation injury, and genetic and epigenetic changes, which may occur, were investigated. Various plant tissues were cryopreserved: dedifferentiated cells and tissues; shoot tips; axillary buds and other meristematic plant parts; dormant buds of woods species, seeds, embryos, and embryo axes; and pollen. Several main methods were used: slow freezing, vitrification, and encapsulation. Liquid nitrogen can also be used to eliminate viruses from the organs (cryotherapy). The existence of endogenous or contaminating microorganisms needs special attention. Furthermore, technological considerations and the use of cryopreservation to maintain transgenic or patented material need to be mentioned.

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Abiotic and Biotic Stress Research in Plants: A Gizmatic Approach of Modern Omics Technologies

Sheikh

Barman

Bhattacharjee

2020

Sustainable Agriculture in the Era of Climate Change

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Cryopreservation and Proteomic Analysis of Vanilla (V. Planifolia A.) Apices Treated With Osmoprotectants

Cited by 6 publications

References 0 publications

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm

Cryopreservation of Plant Cells

Abiotic and Biotic Stress Research in Plants: A Gizmatic Approach of Modern Omics Technologies

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