Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae

Hirano, Tomonari; Godo, Toshinari; Miyoshi, K.; Ishikawa, Kinya; Ishikawa, Masaya; Mii, Masahiro

doi:10.1007/s11816-008-0080-5

Cited by 57 publications

(44 citation statements)

References 17 publications

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“…5). For example, disparities between seed germinability and viability were noted during storage of Phaius tankervilleae seeds (Hirano et al 2009). Germination of this species decreased by 60% over a 6-mo storage period at 4ЊC, yet viability was apparently maintained on the basis of assessment via tetrazolium chloride (the most commonly used vital stain in seed testing).…”

Section: Challenges In Handling Orchid Seedsmentioning

confidence: 99%

“…Seeds of four Dendrobium hybrids, for example, did not survive cryostorage at water contents of 11%-19% (fresh weight), but germination of 150% was achieved following preconditioning of seeds with PVS2 and sucrose over ice (Vendrame et al 2007). Similarly, the germination of seeds of Phaius tankervilleae was maintained at ∼70% during storage in liquid nitrogen for at least 12 mo when seeds were first precultured on a sucrose solution and then treated with PVS2; in the absence of PVS2, no survival was recorded (Hirano et al 2009). For seeds of two species of Dendrobium and the hybrid Vanda Miss Joaquim, which do not survive storage at Ϫ70ЊC, the cryoprotectants mannitol and meso-inositol enhanced survival; after 5 yr of storage, up to ∼30% germination was achieved for Dendrobium crystallinum seeds (Huehne and Bhinija 2011).…”

Section: Vitrificationmentioning

confidence: 99%

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Ex situ Conservation and Cryopreservation of Orchid Germplasm

Merritt

Hay

Swarts

et al. 2014

International Journal of Plant Sciences

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Premise of research. Orchids are among the most enigmatic of plant species. Yet the Orchidaceae comprises more species at risk of extinction than any other plant family. The collection and storage of orchid germplasmprincipally seeds and associated mycorrhizal fungi but also protocorm-like bodies using encapsulation and vitrification techniques-allows for secure ex situ conservation. This article reviews the approaches and techniques used for the ex situ conservation of orchid germplasm, with a focus on seed banking and the use of cryopreservation techniques to improve the longevity of germplasm.Pivotal results. It is increasingly apparent that cryopreservation-the storage of germplasm at ultra-low temperatures (e.g., in liquid nitrogen)-is required for the long-term and low-maintenance conservation of all types of orchid germplasm. For orchid seeds, desiccation tolerance is common, but longevity in storage is poor. Cryopreservation of orchid seeds shows promise, but some complexities in low-temperature storage behavior still require explanation and resolution. The application of more advanced cryopreservation techniques, including encapsulation-dehydration and vitrification, is becoming increasingly common. These techniques provide for the simultaneous storage of orchid propagules with their compatible fungus, while for seeds, vitrification techniques show potential for improving tolerance to the stresses of cryopreservation. Conclusions.A renewed focus on describing the low-temperature storage physiology of orchid seeds to more precisely define the relationship between seed water content, storage temperature, and seed survival is required, as is perhaps the wider adoption of the use of cryoprotectants for seeds. This research, coupled with the development of improved methods of seed viability testing, will support the growing work of germplasm banks to protect orchid biodiversity in the face of habitat loss and potential species extinction.

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Section: Challenges In Handling Orchid Seedsmentioning

confidence: 99%

Section: Vitrificationmentioning

confidence: 99%

Ex situ Conservation and Cryopreservation of Orchid Germplasm

Merritt

Hay

Swarts

et al. 2014

International Journal of Plant Sciences

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“…suzukiana (Hirano et al 2005b), whose mature seeds are recalcitrant, this preservation technique may also be applied to C. kanran. In the case of Phaius tankervilleae, no apparent differences in the deterioration of seed viability and germination were noted between storage periods of 30 min and 12 months at Ϫ196°C (Hirano et al 2009). Therefore, we expect that the Cymbidium seeds used in the present study can be stored over a long time by cryopreservation.…”

mentioning

confidence: 85%

“…Mature seeds of Bletilla striata (Ishikawa et al 1997), Dendrobium (Vendrame et al 2007), Doritis pulcherrima (Thammasiri 2000), Phaius tankervilleae (Hirano et al 2009), and Vanda coerulea (Thammasiri and Soamkul 2007) and immature seeds of B. striata (Hirano et al 2005a) and Ponerorchis graminifolia var. suzukiana (Hirano et al 2005b) have been preserved by this technique.…”

mentioning

confidence: 99%

Wide applicability of cryopreservation with vitrification method for seeds of some Cymbidium species

Hirano

Yukawa

Miyoshi

et al. 2011

Plant Biotechnology

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“…Blume que es una especie nativa de los trópicos, que se distribuye desde el este de Asia a Australia y es clasificada como una especie en peligro de extinción en varios países de su distribución de origen (Hirano et al 2009, Cheng et al 2012.…”

Section: Introductionunclassified

Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)

Duarte¹,

Mangeón²,

Küppers³

et al. 2017

Caldasia

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Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)Seed size and viability: implications on the evolution and conservation of Phaius tankervilleae (Orchidaceae) Argentina. vane.mangeon@hotmail.com, guillo_lq@hotmail.com, procha910@gmail.com, fernandoniella@gmail.com EvElyn RESUMENPhaius tankervilleae es una orquídea terrestre altamente apreciada como ornamental y medicinal, razón por la cual se encuentra en peligro de extinción en algunos países como Australia y Japón. Generar información sobre las dimensiones y el vigor de la semilla es importante para entender aspectos de la evolución de la especie y encontrar métodos de conservación. Este estudio tuvo como finalidad poner a prueba la hipótesis, de que el tamaño y viabilidad de las semillas de P. tankervilleae están positivamente relacionados con el tamaño del fruto. Para realizar el estudio se cosecharon frutos de diferentes tamaños, de los cuales se tomaron muestras de 2,5 mg de semillas por frutos. Se realizó la caracterización morfológica de las semillas mediante observaciones en microscopio. Las semillas de cada tamaño de fruto fueron sometidas a un test de Tetrazolio para determinar viabilidad y se les tomaron mediciones del largo, ancho y volumen tanto de la testa como del embrión. Las semillas con embrión son fusiformes y las vanas son filiformes, el embrión es relativamente pequeño y las semillas de fruto mediano son las que presentan menor espacio de aire. Los resultados confirman la hipótesis planteada, cuanto más pequeño es el fruto menor es el tamaño y la cantidad de semillas viables. Por consiguiente, si se desea tener mayor cantidad de semillas de buena calidad es recomendable cosechar frutos de mayor tamaño.Palabras clave. Embrión, orquídea, tetrazolio, testa, volumen. ABSTRACTPhaius tankervilleae is a terrestrial orchid highly prized as ornamental and medicinal, which is why it is in danger of extinction in some countries such as Australia and Japan. Generating information on seed size and vigor is important in understanding aspects of the evolution of the species and finding conservation methods. This study aimed to test the hypothesis that the size and viability of P. tankervilleae seeds are positively related to fruit size. In order to carry out the study, fruits of different sizes were harvested, from which samples of 2.5 mg of seeds per fruit were taken. Morphological characterization of the seeds was carried out by means of microscope observations. A Tetrazolium test was carried out to determine the viability of seeds and measurements were taken of the length, width, and volume of both the testa and the embryo. Seeds with embryos are fusiform and the empty one are filiform, the embryo consERvación

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Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae

Cited by 57 publications

References 17 publications

Ex situ Conservation and Cryopreservation of Orchid Germplasm

Ex situ Conservation and Cryopreservation of Orchid Germplasm

Wide applicability of cryopreservation with vitrification method for seeds of some Cymbidium species

Tamaño y viabilidad de semillas: implicancias en la evolución y conservación de Phaius tankervilleae (Orchidaceae)

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