Cryopreservation: A tool to conserve date palm in Saudi Arabia

Abstract

The cryostoring of embryogenic tissue of the date palm (Phoenix dactylifera L. cv. Sagai) was examined through dehydrated-encapsulation, vitrification, and vitrification-encapsulation. The most extreme regeneration rate (53.33%) of epitomized, cryostored liquid nitrogen (+LN) treated embryos was observed when pre-embryonic masses were hatched with 0.5 M sucrose for 48 h pursued by 6 h air drying out. The most noteworthy survival rate (80.0%) of epitomized, cryopreserved embryonic cluster came about when calli were hatched with 0.3 or 0.7 M sucrose for 48 h pursued by four hours of lack of hydration, or with 0.5 M sucrose for 48 h without air drying out or with 2 h of air drying out. Following cryopreservation utilizing the embodiment vitrification convention, the most astounding survival (86.7%) as well as the greatest growth (46.7%) was accomplished when the typified vitrified, cryopreserved calli were treated with Vitrification Solution 2 for plants (PVS2) for 60 min at 25 °C. Cryopreservation utilizing the vitrification convention brought about the most extreme recuperation of 53.3%, when vitrified-cryopreserved calli were subjected to PVS2 solution for 30 min at 25 °C. Most extreme (40%) regeneration of vitrified, cryopreserved embryonic calli was seen when these calli were treated with PVS2 solution for 60 min at 25 °C. The outcome got amid this investigation of regrowth after cryopreservation of the cv. Sagai was over the base suitable for a cryo-germplasm bank. Recovery and regrowth were above 30% for all the techniques developed for the cv. Sagai.

Silicon- and Boron-Induced Physio-Biochemical Alteration and Organic Acid Regulation Mitigates Aluminum Phytotoxicity in Date Palm Seedlings

“…The deficiency of B in plants leads to many deformities in plants, such as root growth inhibition; the induction of chlorotic disorders in leaves; a reduction in yield; and the impairment of the cellular components, structure, and composition of the cell wall [ 31 , 32 , 33 ]. Similarly, the alleviation of Al3+-induced stress by the exogenous treatment of B in different plants has been reported previously by many researchers in different plants [ 29 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. Moreover, these studies have suggested that B possibly overcomes the consequences of Al 3+ toxicity by restricting Al binding sites.…”

“…Date palm is reported to be grown in 50% of Oman’s cultivated land and comprises 82% of all the cultivated fruit crops in Oman [ 36 , 37 ]. Metals contamination due to rapid urbanization and anthropogenic activities can negatively affect date palm growth and the quality of fruit [ 38 , 39 ]. During germination and the initial growth stages, date palm plants are reported to be fragile and tender and to adopt different sensing mechanisms to achieve germination under favorable environmental conditions [ 39 ].…”

Cryopreservation of date palm tissue cultures by vitrification and assessment of genetic stability using ISSR analysis

“…In their study on cryopreservation of date palm meristems, they found that perfect PVS2 treatment was 30 min. Similarly, Alansi et al (2019) found that cryopreservation of date palm utilizing the vitrification convention brought about the most extreme recuperation of 53.3%, when vitrified-cryopreserved calli were subjected to PVS2 for 30 min at 25 °C. For cryopreservation of embryogenic callus of date palm, Subaih et al (2007) stated that direct exposure of calli to 100% PVS2 decreased the viability of calli.…”

Cryopreservation of bioflavonoid-rich plant sources and bioflavonoid-microcapsules: emerging technologies for preserving bioactivity and enhancing nutraceutical applications

“…As such, there is LS2, 3, and 4. The purpose of the LS is to prepare the plant specimens biophysically to osmotic stress by exposure to a moderately concentrated osmoticum, prior to severe dehydration with highly concentrated cryoprotectants (CPAs) ( 91 , 100 ).…”